• Korean Journal of Medicinal Crop Science
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• v.7 no.2
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• pp.143-146
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• 1999

The cytotoxicity-guided fractionation of the seed of Plantago asiatica extracts led to the isolation of four compounds, responsible for the cytotoxicity against four human tumor cell lines, i. e., A431 (Human Epidermoid carcinoma), KHOS-NP (Human Osteosarcoma). SNU-1 (Human stomach carcinoma), SNU-C4 (Human large intestine carcinoma). The structure were elucidated by the phsyco chemical data: ${\beta}-sitosterol(C1)$, $cholest-5-en-3{\beta}-ol(C2)$, rutin(C3), $coumarin-7-O-{\beta}-glucopyranoside(C4)$. $IC_{50}$ values of compound C2 were 14.6, 13.5, 10.3, 17.8 ${\mu}g/ml$, and compound C3 and C4 showed activity, having $IC_{50}$ values ranging from 10.3 to 20.14 ${\mu}g/ml$.

• Korean Journal of Pharmacognosy
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• v.6 no.3
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• pp.149-150
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• 1975

Silicagel colum chromatography of the ether extract of the fruits of Angelica decursiva $F_R$. et SAV. gave four crystalline products of coumarins and one steroid. The following furocoumarins and ${\beta}-sitosterol$ were identifed by UV, IR, NMR spectra and physicochemical tests: ${\beta}-sitosterol$, iso-imperatorin, imperatorin, bergapten, umbelliferone.

• Natural Product Sciences
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• v.7 no.3
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• pp.68-71
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• 2001

The phytochemical investigation of Halocharis hispida revealed the presence of 1-heptatriacontanol, ${\beta}-sitosterol$, ${\beta}-sitosterol-3-O-glucoside$, kaempferol, vitexin and isorhamnetin-3-O-galactoside in addition to vanillic, ferulic, isoferulic, syringic and caffeic acids. The different isolated compounds were identified by different physical, chemical, chromatographic and/or spectral methods.

• Natural Product Sciences
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• v.11 no.1
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• pp.13-15
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• 2005

Phytochemical study of the methanolic extract of Flaveria trinervia (Asteraceae) leaves has led to the isolation of three constituents characterised as 3,5,7,4' tetrahydroxy-6-methoxy flavone (6-methoxy kaempferol), oleanolic acid and ${\beta}-sitosterol-{\beta}-D-glucoside$. The identities of the compounds were confirmed by the physical and spectroscopic data and by comparison with authentic samples.

• Biomedical Science Letters
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• v.17 no.3
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• pp.217-223
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• 2011

${\beta}$-sitosterol glucoside exists in a variety of plants and have anti-tumor, anti-microbial, and immunomodulatory activities. Mast cells and eosinophils play important roles in a variety of inflammatory diseases, specifically asthma and atopic dermatitis. In the present study, we used lactose-${\beta}$-sitosterol (L-BS) and investigated the effect of L-BS on inflammatory responses of the human mast cell line, HMC-1 and the human eosinophilic leukemia cell line, EoL-1. In HMC-1 cells, L-BS significantly inhibited cell migration in response to stem cell factor without cytotoxicity. However, the mRNA expression of CC chemokine receptors (CCRs), including CCR1-5, were not altered after L-BS treatment in HMC-1 cells. LPS-induced IL-4 production was also suppressed by L-BS in a dose-dependent manner. In EoL-1 cells, the concentration ranging from 0.1 ${\mu}M$ to 10 ${\mu}M$ of L-BS had no cytotoxicity and had no effect on mRNA expression of major protein-mediators derived from activated eosinophils. However, 100 ${\mu}M$ of L-BS induced the apoptosis of EoL-1 cells in a time-dependent manner. This finding indicates the possibility of L-BS as a potential therapeutic molecule in inflammatory diseases and may contribute to the need to improve current therapeutic drugs.

• Natural Product Sciences
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• v.20 no.4
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• pp.243-250
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• 2014

A simple GC method with a FID detector was developed in order to determine two main compounds (${\beta}$-sitosterol and lupenone) for Adenophorae Radix. ${\beta}$-Sitosterol and lupenone were analyzed by the gradient thermal ramping method. Nitrogen was used as the carrier gas at 108 kPa. The flow rate of gas was 2.0 mL/min; $2{\mu}L$ of filtered sample was injected at a split ratio of 1 : 80. This method was fully validated with respect to linearity, precision, accuracy and robustness. Further, this GC-FID method was applied successfully in order to quantify two compounds in an Adenophorae Radix extract. The GC analytical method for classification analysis was performed by repeated analysis of 59 reference samples in order to differentiate between Adenophora triphylla var. japonica Hara and 14 Codonopsis lanceolata. The results indicate that the GC-FID method is suitable and reliable for the quality evaluation of Adenophorae Radix.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.1
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• pp.25-32
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• 2019

This study was carried out to investigate the changes in fatty acid composition and phytosterol content of the autumn sowing waxy corn during its ripening period for the production of high quality and value-added fresh ears. The content of crude oil in the waxy corn kernels steadily increased with days after pollination(DAP); the crude oil content in Heukjinjuchal (HC) was higher than that in Ilmichal (IC). The composition of fatty acids according to DAP showed a statistically significant change; palmitic and linoleic acid composition gradually decreased, but oleic acid increased, and the composition of unsaturated fatty acids in HC was higher than that in IC. The phytosterol content was increased until 33 DAP, and was 937.7 mg/100g and 867.9 mg/100g in IC and HC, respectively, but there was no statistically significant difference between the varieties. The phytosterol contents in IC varied in the following order: ${\beta}-sitosterol$ > stigmasterol > campesterol, but that in HC varied in the order ${\beta}-sitosterol$ > campesterol > stigmasterol. The campesterol content did not show significant differences between the varieties, but the stigmasterol and ${\beta}-sitosterol$ content was significantly higher in HC than in IC. Palmitic, linolieic, and linolenic acid were significantly correlated with campesterol and ${\beta}-sitosterol$, and stearic acid was positively correlated with stigmasterol. Oleic acid was positively correlated with campesterol and ${\beta}-sitosterol$ but negatively correlated with stigmasterol. As a result, it was suggested that fatty acids may affect the variation in phytosterol contents during the ripening period in waxy corn.

• Natural Product Sciences
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• v.10 no.5
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• pp.237-239
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• 2004

Mallotus nepalensis (Euphorbiaceae) is a small tree found in central and eastern Himalayas, Nepal, Wallich and Sikkim. The 90% ethanolic extract of Mallotus nepalensis exhibited mild CNS depressant activity. Four compounds, lupeol (1), ${\beta}-sitosterol$ (2), ursolic acid (3) and ${\beta}-sitosterol-\;{\beta}-D-glucoside$ (4) were isolated from the 90% ethanolic extract of this plant of which 1, 3 and 4 are being reported for the first time from this species.

• Applied Chemistry for Engineering
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• v.17 no.4
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• pp.375-380
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• 2006

In the paper, lipophilic derivatives of $\beta$-sitosterol, which are known to have a potential to reduce blood cholesterol level, were synthesized by the esterification of $\beta$-sitosterol and fatty acids. When the esterification reactions using stearic acid, oleic acid or linoleic acid as fatty acids were carried out in the presence of an acidic catalyst, the reaction for unsaturated fatty acids such as oleic acid and linoleic acid afforded a significant amount of side products which may be produced by oxidation of unsaturated groups. On the other hand, esterification reactions in the presence of dehydrating agents and a basic catalyst gave pure products regardless of the nature of fatty acids. The solubilities of lipophilic derivatives of $\beta$-sitostero to organic solvents and edible oil were observed to increase as the degree of unsaturation of fatty acids increases.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.1
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• pp.39-46
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• 2020

Alzheimer's disease (AD) is the most common neurodegenerative disorder causing dementia worldwide, and is mainly characterized by aggregated β-amyloid (Aβ). Increasing evidence has shown that plant extracts have the potential to delay AD development. The plant sterol β-Sitosterol has a potential role in inhibiting the production of platelet Aβ, suggesting that it may be useful for AD prevention. In the present study, we aimed to investigate the effect and mechanism of β-Sitosterol on deficits in learning and memory in amyloid protein precursor/presenilin 1 (APP/PS1) double transgenic mice. APP/PS1 mice were treated with β-Sitosterol for four weeks, from the age of seven months. Brain Aβ metabolism was evaluated using ELISA and Western blotting. We found that β-Sitosterol treatment can improve spatial learning and recognition memory ability, and reduce plaque load in APP/PS1 mice. β-Sitosterol treatment helped reverse dendritic spine loss in APP/PS1 mice and reversed the decreased hippocampal neuron miniature excitatory postsynaptic current frequency. Our research helps to explain and support the neuroprotective effect of β-Sitosterol, which may offer a novel pharmaceutical agent for the treatment of AD. Taken together, these findings suggest that β-Sitosterol ameliorates memory and learning impairment in APP/PS1 mice and possibly decreases Aβ deposition.