• Title/Summary/Keyword: ${\beta}-isopropylmalate$ dehydrogenase

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Cloning and Expression of Leu 2 Gene (${\beta}-isopropylmalate$ dehydrogenase) from the Basidiomycete Flammulina velutipes in E. coli (팽나무버섯 균사체에서 ${\beta}-isopropylmalate$ dehydrogenase(Leu 2) gene 의 cloning 및 E. coli에서 발현)

  • Byun, Myung-Ok;Yoo, Young-Bok;Go, Seung-Joo;You, Chang-Hyun;Cha, Dong-Yul;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.1
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    • pp.35-38
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    • 1989

  • Gene libraries of DNA from Flammulina velutipes were constructed using Escherichia coli plasmid pBR 322. Leu 2 gene coding ${\beta}-isopropylmalate$ dehydrogenase from F. velutipes was cloned by complementation of leucine requiring mutant of E. coli. The size of inserted DNA fragment of this clone is about 1 Kbp. The fragment has Bam H1 and Ava 1 restriction sites.

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Transformation of the ${\beta}-Isopropylmalate$ Dehydrogenase Gene of Flammulina velutipes into Pleurotus florida (팽나무버섯 Leu 2 gene(${\beta}-isopropylmalate$ dehydrogenase)의 사철 느타리버섯 형질전환)

  • Byun, Myung-Ok;Yoo, Young-Bok;Go, Seung-Joo;You, Chang-Hyun;Cha, Dong-Yul;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.1
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    • pp.27-30
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    • 1989

  • F. velutipes Leu 2 gene (${\beta}-isopropylmalate$ dehydrogenase gene) was used for transformation of P. florida leucine requiring auxotrophic mutant P101. Transformation frequency was very low but the transformed colony can grow on minimal medium very slowly. Transformation was identified by Southern hybridization and reverse transformation into E. coli using chromosome DNA isolated from transformed P. florida.

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