• ETRI Journal
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• v.39 no.1
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• pp.21-29
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• 2017

This paper addresses the problem of unsupervised speech separation based on robust non-negative matrix factorization (RNMF) with ${\beta}$-divergence, when neither speech nor noise training data is available beforehand. We propose a robust version of non-negative matrix factorization, inspired by the recently developed sparse and low-rank decomposition, in which the data matrix is decomposed into the sum of a low-rank matrix and a sparse matrix. Efficient multiplicative update rules to minimize the ${\beta}$-divergence-based cost function are derived. A convolutional extension of the proposed algorithm is also proposed, which considers the time dependency of the non-negative noise bases. Experimental speech separation results show that the proposed convolutional RNMF successfully separates the repeating time-varying spectral structures from the magnitude spectrum of the mixture, and does so without any prior training.

• Journal of Korean Ophthalmic Optics Society
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• v.5 no.1
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• pp.101-105
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• 2000

This study was to investigate the effect of EEG wave type on visual cortex of visual target according to position of fixation point on the Korean. Visual evoked potential system used the BIO-Pag and recorded to 586 computer. The illumination was 500 lux and the visual target was red light dot of 3 cm size. The results of the convergence and divergence as follows: The visual stimulation waves on the visual cortex have about 70% of delta wave, about 10% of beta wave, about 9% of theta wave and about 7% of alpha wave respectively. The convergence state was much more appeared the fast wave on the comparative of the divergence. Therefore, the convergence state was much more producted the beta and alpha wave on the comparative of the divergence. On the other hand, on the convergence and divergence, the histogram amplitude of EEG wave appeared almost the non-Gaussian shape. According to the phase analysis of amplitude of EEG wave almost all type was linear shape.

• Journal of the Earthquake Engineering Society of Korea
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• v.9 no.6 s.46
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• pp.1-12
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• 2005

For a shear-deformable beam-column element subjected io non-conservative forces, equations of motion and a finite element formulation are presented applying extended Hamilton's principle. The influence of non-conservative force's direction parameter, internal and external damping forces, and shear deformation and rotary inertia effects on divergence and flutter loads of Beck's columns are intensively investigated based on element stiffness, damping and mass matrixes derived for the non-conservative system.

• Journal of Korea Water Resources Association
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• v.38 no.4 s.153
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• pp.271-280
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• 2005

The goal of this study is to recommend a new type of stage-discharge rating curve ($Q=p(h-e)^{\beta}-{\gamma}$) useful for satisfying divergence, and one other seemingly irresolvable problem related to exited rating curves, while also extending this rating curve model. The problem of divergence is that during the finding of the CZF (cease-to-zero flow) parameter e and while minimizing the sum of total errors of the estimated curve, the exponential parameter ${\beta}$ become an abnormally large value. The insoluble problem is that when the value e is greater then the recorded minimum at the gauged stage, it is impossible to have a negative logarithm value (h-e). The two problems above can be satisfied by adapting the control value ${\gamma}$, which affects the reduction of ${\gamma}$ and gives us the possibility of controlling (h-e) over zero. The study results show that the effects of parameter ${\gamma}$ are very similar to that of e when conducting physical and sensitivity analyses. This system can be used towards developing a new stage-discharge rating curve for river discharge, for use in evaluating the acceptability of existing stage-discharge rating curves generated by using hydrologic analyses at all stations.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.670-676
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• 2000

Several clones obtained from Serratia marcescens stimulated E. coli TP2139 (${\Delta}lac, \;{\Delta} crp$) cells to use maltose as a carbon source. The crp gene clone, pCKB12, was confirmed to stimulate the $\beta$-galactosidase activity, by Southern hybridization [31]. The nucleotide sequence of the crp region consisting of 1,979 bp was determined. The sequencing of the fragment led to the identification of two open reading frames: One of these, the crp gene, encoded 210 amino acid and the other encoded a truncated protein. The S. marcescens and E. coli crp genes showed a higher degree of divergence in their nucleotide sequence with 120 changes, however, the corresponding amino acid sequences showed only two amino acid differences. Yet, an analysis of the amino acid divergence revealed that the catabolite gene activator protein, the crp gene product, was the most conserved protein observed so far. Using a crp-lac protein fusion, it was demonstrated that S. marcescens CRP could repress its own expression, probably via a mechanism similar to that previously described for the E. coli crp gene.

• The Journal of Dong Guk Oriental Medicine
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• v.7 no.2
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• pp.141-148
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• 1999

A simple and rapid FoLT(formamide low temperature)-PCR, whereby human genomic DNA from blood can be amplified without DNA preparative stps, is described using human insulin genes. By applicatin of FoLT-PCR in human insulin genes, intragenic polymorphism in non-coding regions of the human insulin gene was shown after amplification and analysis by restriction enzyme digestion. All nucleotide sequences were the same as the reported, and four necleotides, at 4 different positions were polymorphic, and polymorphic alleles ${\alpha}4$, ${\alpha}5$, ${\alpha}6$, and ${\beta}2$ were identified. The new alleles were originated from homologous recombination between the ${\alpha}1$ and ${\beta}1$ alleles, and the alleles were founded in heterozygotes only. Although allele ${\alpha}1$ was dominant, the new alleles and ${\beta}1$ were recessive. From the results, it was suggested that the new method of FoLT-PCR was highly applicable in genetic variation analysis.

• Korean Journal of Microbiology
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• v.48 no.4
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• pp.319-324
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• 2012

In this study, we investigated molecular divergences and phylogenetic characteristics of the 16S ribosomal RNA (rRNA) and RNA polymerase beta subunit (rpoB) gene sequences from the order Oscillatoriales (Cyanobacteria). The rpoB of Oscillatoriales showed higher genetic divergence when compared with those of 16S rRNA (p-distance: rpoB=0.270, 16S=0.109), and these differences were statistically significant (Student t-test, p<0.001). Phylogenetic trees of 16S rRNA and rpoB were generally compatible; however, rpoB tree clearly separated the compared Oscillatoriales taxa, with higher phylogenetic resolution. In addition, parsimony analyses showed that rpoB gene evolved 2.40-fold faster than 16S rRNA. These results suggest that the rpoB is a useful gene for the molecular phylogenetics and species discrimination in the order Oscillatoriales.

• Korean Journal of Microbiology
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• v.46 no.3
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• pp.296-302
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• 2010

Microcystis (Cyanobacteria, Chroococcales) is one of the green tide-causing organisms in freshwaters, and some species produce microcystin that is hepatotoxin. In the aspects of freshwater quality controls and health concerns, therefore it is necessary to manage the harmful organisms. In the present study, RNA polymerase beta subunit (rpoB) gene sequences of Microcystis were determined and characterized in order to use a potential marker for the molecular detections of the species. Microcystis rpoB showed high divergences of DNA similarity and genetic distances when compared with those of 16S rRNA, and the molecular differences were statistically significant (Student t-test, p<0.05). Parsimony analyses showed the rpoB gene evolves more than 2-fold faster than 16S rRNA. In addition, phylogeny of the rpoB gene separated each M. aeruginosa strain more clearly compared with a 16S rRNA tree. This study found that the order Chroococcales, including Microcystis, has approximately two rRNA operons and single copy of the rpoB gene in their chromosomes. These results suggest that the rpoB gene is a useful marker for the molecular phylogenetics and the detection of Microcystis.

• The Journal of the Korean Society for Microbiology
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• v.34 no.6
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• pp.561-571
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• 1999

Diagnosis of mycobacterial infection is dependent upon the isolation and identification of causative agents. The procedures involved are time consuming and technically demanding. To improve the laborious identification process mycobacterial systematics supported by gene analysis is feasible, being particularly useful for slowly growing or uncultivable mycobacteria. To complement genetic analysis for the differentiation and identification of mycobacterial species, an alternative marker gene, rpoB encoding the ${\beta}$ subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 52 reference strains of mycobacteria including Mycobacterium tuberculosis H37Rv (ATCC 27294) and clinical isolates by the PCR. The nucleotide sequences were directly determined (306 bp) and aligned using the multiple alignment algorithm in the MegAlign package (DNASTAR) and MEGA program. A phylogenetic tree was constructed with a neighborhood joining method. Comparative sequence analysis of rpoB DNA provided the basis for species differentiation. By being grouped into species-specific clusters with low sequence divergence among strains belonging to same species, all the clinical isolates could be easily identified. Furthermore RFLP analysis enabled rapid identification of clinical isolates.

• Journal of the Korean Vacuum Society
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• v.1 no.1
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• pp.88-95
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• 1992

Abstract-Using a high resolution low energy electron diffraction(HRLEED), we report an exponent study of 2d continuous phase transition from an ordered ~ ( 2 x 1 )ox ygen overlayer on a W(110) surface. Temperature dependence of a (% 0) superlattice diffraction spot, characteristic of the p(2X 1) structure, shows power-law like divergence of the susceptibility and the fluctuation correlation length at T,=708.765 K. By fitting the intensities as well as the line-shapes, we obtained exponents P=0.19* 0.05, y=1.48+ 0.34, v= 1.23i 0.27 and q=0.38+ 0.12. The non-universal character of the exponents are understood in terms of a 2d XY model with cubic anisotropy as suggested previously.