• 한국발생생물학회지:발생과생식
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• 제20권2호
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• pp.91-99
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• 2016

Lipopolysaccharide (LPS), an endotoxin, elicits strong immune responses in mammals. Several lines of evidence demonstrate that LPS challenge profoundly affects female reproductive function. For example, LPS exposure affects steroidogenesis and folliculogenesis, resulting in delayed puberty onset. The present study was conducted to clarify the mechanism underlying the adverse effect of LPS on the delayed puberty in female rats. LPS was daily injected for 5 days ($50{\mu}g/kg$, PND 25-29) to treated animals and the date at VO was evaluated through daily visual examination. At PND 39, animals were sacrificed, and the tissues were immediately removed and weighed. Among the reproductive organs, the weights of the ovaries and oviduct from LPS-treated animals were significantly lower than those of control animals. There were no changes in the weights of uterus and vagina between the LPS-treated and their control animals. immunological challenge by LPS delayed VO. Multiple corpora lutea were found in the control ovaries, indicating ovulations were occurred. However, none of corpus luteum was present in the LPS-treated ovary. The transcription level of steroidogenic acute regulatory protein (StAR), CYP11A1, CYP17A1 and CYP19 were significantly increased by LPS treatment. On the other hand, the levels of $3{\beta}$-HSD, $17{\beta}$-HSD and LH receptor were not changed by LPS challenge. In conclusion, the present study demonstrated that the repeated LPS exposure during the prepubertal period could induce multiple alterations in the steroidogenic machinery in ovary, and in turn, delayed puberty onset. The prepubertal LPS challenge model used in our study is useful to understand the reciprocal regulation of immune (stress) - reproductive function in early life.

• 한국자기공명학회논문지
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• 제4권2호
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• pp.74-81
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• 2000

Saccharomyces cerevisiae Dna2 protein has biochemical activities: DNA-dependent ATPase, DNA helicase and DNA nuclease and is essential for cell viability. Especially, Pro$\^$504/ is determined as an important residue in ATPase, helicase, and nuclease activity. We synthesized and determined the three-dimensional solution structure of N-terminal domain comprising residues of Val$\^$501/ -_Phe$\^$508/ (Dna2$\^$pep/) using two-dimensional $^1$H-NMR and dynamical simulated annealing calculations. On the basis of a total of 44 experimental restraints including NOEs, $^3$J$\_$$\alpha$$\beta$/ and $^3$J$\_$$\alpha$$\beta$/ coupling constants, the solution structures of Dna2$\^$epe/ were calculated with the program CNS. The 23 lowest energy structures were selected out of 50 final simulated-annealing structures. The atomic RMSDs of the final 23 structures fur the individual residues were calculated with respect to the average structure. The mean RMSDs for the 23 structures were 0.042 nm for backbone atoms and 0.316 nm for all heavy atoms, respectively. The Ramachandran plot indicates that the $\Phi$, Ψ angles of the 23 final structures are properly distributed in energetically acceptable regions. Solution structure of Dna2$\^$pep/ showed a single unique turn spanning residues of Asn$\^$503/ Val$\^$506/.

• 대한한의학회지
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• 제26권1호
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• pp.161-173
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• 2005

Objective : The water extract of Seokchangpowonji-san (SWS) has traditionally been used for treatment of dementia in oriental medicine. However, little is known about the mechanism by which the water extract of SWS rescues cells from neurodegenerative disease such as Alzheimer's disease. Methods & Results: This study was designed to investigate the protective mechanisms of SWS on $\beta-amyloid$ or $H_2O_2$-induced$ cytotoxicity in neuro 2A cells. $H_2O_2$ markedly decreased the viability of neuro 2A cells, which was characterized by apparent apoptotic features such as membrane blebbing as well as fragmentation of genomic DNA and nuclei. However, the water extract of SWS significantly reduced $H_2O_2-induced$ cell death and apoptotic characteristics through reduction of intracellular peroxide generation. Also, the. extract prevented the mitochondrial dysfunction including the disruption of mitochondria membrane permeability transition (MPT) and the modulation in expression of Bcl-2 family proteins in $H_2O_2­treated$ neuro 2A cells. Furthermore, pretreatment with SWS inhibited the activation of caspase-3, and in turn, degradation of ICAD/DFF45 was completely abolished in $H_2O_2-treated$ cells. Conclusion: Taken together, the data suggest that the protective effects of the water extract of SWS against $\beta-amyloid$ induced oxidative injuries may be achieved through modulation of mitochondrial dysfunction.

• 한국실내디자인학회논문집
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• 제24권5호
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• pp.99-107
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• 2015

The purpose of this study is to analyze the change of visual stimulus of users to the space through the experiment of EEG and the satisfaction of users depends on the lighting. To do that, the results measured with EEG experiment focusing on Beta ${\beta}$ were compared to each other to figure out difference in the changes of the activation of human brain on lighting's situation as the lighting off and on in the same space. The difference in the results was verified according to the characteristic of users which classified with 4 types of the spatial sensitivities. The results of this study are as following. Firstly, the spacial sensitivity of user is to communicate well with the different senses with stimulus through interaction among the elements. At this time, the brain plays a major role in build the spacial sensitivity of users as the place to make form. Secondly, there are the differences in the activation of brain depends on lighting situation even in the same space. The stimulus into the brain became generally stronger in images with lighting on than off. Especially, the response in the occipital lobe which connected with the visual center turn out strongly in the image of 'modern natural'. Because the visual stimulus interact well with the bright color, the reflectional texture and the rough texture painted the dark color. Thirdly, the satisfaction of users changed with lighting in the space. But we could know that the satisfaction of users isn't be related to the visual stimulus through the results of this study. Finally, there isn't the difference in the activation degree of brain according to the characteristic which are preference of users into 4 types of the spatial sensitivity through the results came from ANCOVA(analysis of covariance) with SPSS Program 22.

• 한국환경농학회지
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• 제30권4호
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• pp.367-376
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• 2011

논 토양의 미생물 생태 다양성을 조사하기 위한 효과적인 방법으로 qRT-PCR을 적용하고자 본 연구를 수행하였다. 논 토양 미생물의 gDNA를 분리하기 위하여 Mo Bio kit를 사용한 효과적이고 안정적인 gDNA 분리 방법을 확립하였다. 논 토양 미생물 다양성을 qRT-PCR로 검출하기 위하여 bacteria를 세분한 ${\alpha}$-Proteobacteria, ${\beta}$-Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes 다섯 가지 문과 전체 bacteria, 전체 fungi를 구분할 수 있는 특이 primer set을 선정하여 다양한 조건의 시험을 통하여 최종 조건을 확립하였으며 재현성 실험을 통하여 방법의 유의성을 검증하였다.

• 한국산학기술학회논문지
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• 제15권5호
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• pp.2695-2702
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• 2014

본 연구의 목적은 중소병원 간호사의 간호전문직관, 직무만족도 및 이직의도와의 관계를 파악하기 위해 2011년 10월부터 2012년 6월까지 "I"시에 위치한 100-400병상 규모의 8개 중소병원에 근무하는 간호사 240명을 대상으로 설문조사하였다. 대상자의 평균 간호전문직관 점수는 $3.23{\pm}.44$, 평균 직무만족 점수는 $2.81{\pm}.47$점, 평균 이직의도 점수는 $2.70{\pm}.87$점으로 대상자의 일반적 특성과 직무 관련 특성 중 유의한 차이가 있었던 변수는 연령, 결혼상태, 임상경력, 근무형태, 밤 근무 횟수였고, 대상자의 이직의도는 직무만족과 양의 상관관계, 간호전문직관 과의 관계는 정적인 상관관계를 보였다. 이직의도를 설명하는 주요 변수는 직무만족도, 임상경력, 연령이며, 이중 직무만족도(${\beta}$=.533)가 다른 관련요인보다 가장 영향력이 많은 것으로 나타났다. 본 연구결과 여전히 직무만족도가 중소병원 간호사의 이직의도에 영향을 주는 주요 변수인 것이 확인 되었고, 이로써 중소병원 간호사의 이직의도를 이해하고, 그 수를 줄이는데 있어 중소병원 간호사에 대한 직무 만족도에 관해 계속적으로 연구하는 것이 필요하겠다.

• 한국융합학회논문지
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• 제10권5호
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• pp.111-117
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• 2019

본 연구는 차량 사고에서 병원 전 응급의료 대응시간을 단축시키기 위한 방안을 모색한 융합연구이다. 연구방법은 한국형 교통사고 심층조사 분석 체계(Korea In-Depth Accident Study)에서 2011년 1월 1일부터 2016년 7월 30일까지 3개의 응급의료센터에 119구급대로 내원한 차량 탑승자 353명을 대상으로 날씨, 도로유형, 사고유형, 구조대 출동 여부를 활용하여 병원 전 응급의료 대응시간에 대해 요인 분석하였다. 연구결과에서 고속도로는 병원 전 응급의료 대응시간을 가장 많이 소요하였고 전체시간에 영향을 주는 요인으로 확인되었다(${\beta}=.543$, p<.001). 따라서 고속도로에서 소요되는 시간을 단축시키기 위해 고속도로 119구급대의 운영과 비상회차로의 적극적인 사용, 개별 장치를 부착한 자동 긴급구조신호 서비스의 제공을 제시하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1186-1194
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• 2019

Objective: The influence of broiler carcass scalding conditions on chicken breast meat quality parameters was investigated. Methods: Two hundred and seventy Cobb broiler chickens from 42 to 48 days old were slaughtered according to the standard industry practice and scalded in five temperature/time combinations-$T_1$, $54^{\circ}C/210s$; $T_2$, $55^{\circ}C/180s$; $T_3$, $56^{\circ}C/150s$; $T_4$, $57^{\circ}C/120s$; $T_5$, $58^{\circ}C/90s$. Results: Scalding temperature increase resulted in higher values of external and ventral lightness and in protein functionality reduction-determined by emulsification capacity and protein denaturation-in chicken breast fillets 24 h post-mortem. Protein secondary structures had conformational changes, with a decrease of the ${\alpha}$-helix and an increase of the ${\beta}$-sheet and ${\beta}$-turn proportions, mainly in $T_1$ and $T_5$ samples, determined by Fourier-transform infrared spectroscopy in an attenuated reflectance mode analysis. The chemical composition, pH, water holding capacity and Warner-Bratzler shear force did not differ among the treatments. In the fatty acid profile, the 18:1n-9 was lower in $T_5$, which suggested that the high scalding-temperature could have caused the lipid oxidation. The values of the polyunsaturated fatty acids (PUFA), such as 22:2, 20:4n-6, and 22:6n-3, were highest in the $T_5$, thus being related to the phospholipid cellular membrane collapse in this experimental condition and subsequent release of these PUFA. Conclusion: Intermediate scalding-parameters avoided the negative changes in the chicken meat quality.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.938-945
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• 2005

For elucidating excessive growth of biofilm that subsequently leads to the clogging problem in a small town's sewer lines of Wisconsin, the FISH method was employed. At the beginning of the simulated experiments, ${\beta}$-subclass proteobacteria prevailed in runs fed with industrial wastewater, while ${\gamma}$-subclass proteobacteria dominated in runs with domestic wastewater. However, the bacterial community structure changed significantly over six weeks; Cytophaga-Flavobacterium (CF)­group bacteria dominated in most runs fed with the small town's wastewater regardless of their source, while CF-group decreased strongly in run fed with domestic sewage from another city (Madison). It was also microscopically confirmed that most of those clogging materials was toilet tissue, which in turn may lead to vigorous growth of cellulose-degrading CF-group bacteria. This dominant presence of CF-group bacteria in the small town's sewer indicates that the main constituent of biofilm, toilet tissue (cellulose) in sewage, might have induced the unique pattern of their microbial community structure. Therefore, it suggests that molecular technique is useful for monitoring the clogging problems in sewer lines.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2003년도 International Meeting on Information Display
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• pp.1021-1023
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• 2003

We studied field-emission characteristics of CNTs under various pre-treatment with $NH_{3}$ plasma on the substrate. The turn-on electric field is the lowest value and field enhancement factor (${\beta}$) is he highest value in CNTs pre-treated by $NH_3$ plasma (80 W and 5 min). The field-emission property of CNTs grown on the Ta substrate is slightly better than on the W substrate.