• Journal of Plant Biotechnology
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• 제36권1호
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• pp.64-67
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• 2009

순무 뿌리로부터 활성 물질을 분리 동정하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었으며, EtOAc분획과 n-BuOH 분획에 대해 silica gel 및 ODS column chromatography를 실시하여 4종의 이차대사산물을 분리 정제하였다. $^1H-NMR,\;^{13}C-NMR$, DEPT spectrum 및 mass spectrum등을 통하여 4- (methoxymethyl)phonol(1), ${\alpha}$- methoxy-2, 5- furandimethanel (2), phenyl-${\beta}$-glucopyranoside(3) 및 2-phenylethyl-${\beta}$-D -glucopyranoside (4)로 구조를 결정하였다. 이 화합물들은 순무에서는 처음 분리되었다.

• Applied Biological Chemistry
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• 제42권2호
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• pp.170-175
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• 1999

국내 식물자원으로부터 천연 항산화물질을 탐색하기 위하여 항산화활성이 기대되는 38종의 식물을 MeOH 수용액으로 추출한 후, EtOAc와 물로 분배, 추출하였다. 각 추출물에 대하여 DPPH radical 소거활성을 검정하였고, 이중에 작약을 포함한 13종의 식물에서 활성이 확인되었다. 작약뿌리로부터 항산화물질을 분리하기 위하여 80% MeOH 수용액으로 추출, 농축하였고, 이를 EtOAc, n-BuOH 및 물로 계통분획하였다. 활성이 확인된 n-BuOH과 EtOAc층에 대하여 활성을 추적해가며 column chromatography를 반복하여 3종의 활성물질을 분리하였다. 이들의 화학구조를 NMR 등의 spectral data를 해석하여 (+)-catechin, $1,2,3,4-tetragalloyl-6-digalloyl-{\beta}-D-glucose$ 및 $1,2,3,4,6-pentagalloyl-{\beta}-D-glucose$로 동정하였다.

• 한국수정란이식학회지
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• 제12권3호
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• pp.277-282
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• 1997

The purpose of this experiment was to determine the effects of thiol compounds, $\beta$-mercaptoethanol($\beta$-ME) and cystearrone with buffalo rat liver cell(BRLC) co-culture on the development and intracellular glutathione(GSH) concentrations of bovine embryos produced by in vitro inaturation(IVM) and in vitro fertilization(IVF). Bovine IVM /IVF embryos developed to 2~8 cell stage were co-cultured with BRLC in GRlaa with or without thiol compounds. The developmental rate beyond morulae stage in CRlaa containing 0, 10,25 and 50$\pi$M $\beta$-ME with BRLG were 63.0, 74.0, 72.3 and 77.1%, respectively. And the developmental rate with 0, 25, 50 and 75$\pi$M cystearnine with BRLC were 69.6, 77.6, 81.0 and 76.8%, respectively. The developmental rate beyond morulae stage of GRlaa containing thiol compound with BRLG group was higher than that of control group. The intracellular GSH concentrations of blastocysts cultured for 5 days in GRlaa containing 0 and 50$\pi$M $\beta$-ME or cysteamine with BRLG were 81.2 and 86.4, 83.2 and 84.2pM, respectively. The intracellular GSH concentrations of blastocysts in GRlaa containing thiol compounds with BRLG was slightly higher than that of control group The cell numbers of blastocysts were not difference in all experimental groups. These results indicate that thiol compounds with BRLG co-culture was increased the percentage of developed into morulae and blastocysts, and intracellular GSII concentrations of blastocysts embryos.

• 한국임상수의학회지
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• 제36권5호
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• pp.259-265
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• 2019

This study was designed to investigate the effects of alpha-glucosyl rutin (G-rutin) and its comparative effects with other antioxidants (glutathione: GSH, catalase: CATA and beta-mercaptoethanol : ${\beta}ME$) on dog sperm freezing. In the first experiment (E1), the spermatozoa were diluted in freezing extender supplemented with 0 (control), 0.001, 0.01, or 0.1% G-rutin and frozen using liquid nitrogen ($LN_2$). The progressive motility, reactive oxygen species (ROS) level and apoptosis of spermatozoa were assessed after sperm thawing at $37^{\circ}C$ for 25 sec. In the second experiment (E2), 0.1% G-rutin group was compared with 10 mM ${\beta}-ME$, $5{\mu}M$ GSH and $50{\mu}M$ CATA groups by assaying progressive motility, viability and gene expression of Bcl-2 and SMCP after sperm freezing and thawing. In E1, 0.1% G-rutin group showed higher (P < 0.05) post-thaw progressive motility and lower (P < 0.05) ROS levels. In E2, the expressions of SMCP in G-rutin group were higher (P < 0.05) than in CATA group while Bcl-2 expression of G-rutin group was higher (P < 0.05) than ${\beta}-ME$ and CATA groups. However, there were no significant differences in progressive motility and viability. Therefore, we suggest that G-rutin can be used as a potentially antioxidative supplement in dog sperm freezing extender on the basis of gene expression related to motility and apoptosis as well as ROS level.

• 한국식품영양과학회지
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• 제38권7호
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• pp.832-838
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• 2009

저온 진공 공정으로 건조된 비트를 유기용매로 추출하여 이들 비트 추출물 및 분획물들에 의한 인체 결장암 및 위암세포에 대한 증식 및 세포 내 활성산소종 억제 효과에 대해 검토하였다. 인체 결장암세포(HT-29)의 경우, A+M 추출물은 0.5 mg/mL 첨가농도에서 65%의 암세포 증식 억제효과를 나타내었고(p<0.05), MeOH 추출물은 A+M 추출물과 비교하였을 때 전반적으로 낮은 활성을 나타내었음을 관찰하였다. 건조 비트 추출물을 hexane, 85% aq. MeOH, BuOH, water로 다시 추출하여 얻어진 각각의 분획물들을 농도별로 처리하였을 때 특히 hexane 및 85% aq. MeOH 분획물은 낮은 농도에서부터 활성을 나타내어 0.5 mg/mL 농도에서 76% 이상의 높은 암세포 증식 억제효과(p<0.05)를 나타내었다. 인체 위암세포(AGS)에 대한 결과에서 A+M 추출물은 인체 결장암세포(HT-29)의 결과와 비교했을 때 암세포증식 억제효과가 높았고, MeOH 추출물은 A+M 추출물에 비해 낮은 활성을 나타내었으나 앞서 인체 결장암세포(HT-29)에 비해 다소 높은 암세포 성장 억제효과를 보였다. 건조 비트 추출물의 분획물들을 농도별로 인체 위암세포(AGS)에 처리했을 때 hexane과 85% aq. MeOH 분획물은 앞서의 인체 결장암세포(HT-29)의 결과와 유사하게 낮은 농도에서부터 활성이 나타나 0.25 mg/mL 이상의 농도에서 77% 이상의 억제효과가 나타났다. 인체 섬유육종세포(HT1080) 내 활성산소종 억제 실험에서 건조 비트 A+M 및 MeOH 추출물은 농도 의존적으로 측정시간 120분 동안 $500{\mu}M$ $H_2O_2$만을 처리한 control군에 비해 세포 내 활성산소종을 크게 억제시켰다. 건조 비트 분획물들을 인체 섬유육종세포(HT1080)에 처리했을 때 첨가농도 0.1 mg/mL로 처리하였을 때 BuOH 분획물을 제외한 기타 분획물들의 경우 control군보다는 낮은 수치를 나타내어 우수한 항산화 활성을 보였다. 첨가농도 0.25 mg/mL에서 85% aq. MeOH 분획물은 세포 내 활성산소종을 감소시키는데 우수한 능력을 나타내었고 hexane과 BuOH 분획물은 $500{\mu}M$ $H_2O_2$만을 처리한 control군에 비해서는 활성산소종을 감소시켰으나 blank 군보다는 높았다. 따라서 세포 내 활성산소종 감소에 의한 항산화 활성은 85% aq. MeOH 분획물에서 높았고 water 분획물의 효과가 가장 낮았음을 살펴 볼 수가 있었다.

• Journal of Radiation Protection and Research
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• 제5권1호
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• pp.7-10
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• 1980

With the conceptual definition of the quotient(${\beta}$) of absorbed dose and the collision part of kerma for photon beams, the procedure of computing ${\beta}$ is briefly described. A series of calculations of ${\beta}$ was carried out for photons of 0.4, 0.5, 1 and 2 MeV in polystyrene, carbon, air and aluminum. Resultant values are tabulated and evaluated.

• 약학회지
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• 제48권1호
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• pp.65-69
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• 2004

The chromatographic separation of MeOH extract of the aerial parts of Aster glehni (Compositae) led to the isolation of three sesquiterpenes, two sterols and four terpenes. Their structures were determined to be $\beta$-amyrin acetate (1), phytol (2), alismol (3), $\alpha$-tocopheryl quinone (4), $\alpha$-spinasterol (5), 10-O-methyl alismoxide (6), alismoxide (7), and 3-O-(6'-O-palmitoyl-$\beta$-D-glucosyl)-spinasta 7,22-diene (8) by physicochemical and spectroscopic methods . These compounds (1-8) were first isolated from the Aster glehni.

• 생약학회지
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• 제20권3호
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• pp.145-146
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• 1989

A furostanol glycoside, $mp\;190{\sim}198^{\circ}$, was isolated from the MeOH extract of Smilax china rhizomes. The structure was established as a mixture of $26-O-{\beta}-_D-glucopyranosyl-(25R)-22-methoxy-furost-5-en-3\beta,26-diol\;3-O-\alpha-_L-rhamnopyranosyl-(1\rightarrow2)-\beta-_D-glucopyranoside$ and its 22-hydroxy derivative on the basis of spectral data and chemical correlations.

• 대한약리학회지
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• 제31권1호
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• pp.17-26
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• 1995

8주, 18주, 그리고 32주된 Spontaneously Hypertensive Rats (SHR)과 Wistar-Kyoto Rats(WKY)과의 blood pressure(혈압)를 측정하여본 결과 SHR 그룹이 WKY에 비해 19에서 70 mmHg 차이로 SHR 그룹이 WKY group에 비하여 혈압이 높았다. 18주된 SHR과 WKY에서 제 3 뇌실내 (intraventricular)로 투여된 morphine과 ${\beta}-endorphin$의 진통작용을 검색하여 보았다. WKY group에 비하여 SHR group에서 뇌실내로 투여된 ${\beta}-endorphin$은 진통작용에 있어서 상승작용 (potentiation)을 보임을 발견하였고 뇌실내로 투여된 morphine은 SHR group에서 약간만 상승작용을 보였다. SHR과 WKY group간에 opioid의 진통작용에 있어서 중요한 역할을 하는 Midbrain과 Medulla (pons), 그리고 spinal cord (척수)의 lumbar부위의 $[Met^5]-enkephalin$과 proenkep-halin A mRNA level을 측정하여 보았다. SHR과 WKY group간의 $[Met^5]-enkephalin$과 proenkephalin mRNA의 양은 별로 차이를 보이지 않았다. 이러한 결과로 미루어 볼때 SHR group에서 뇌실내로 투여된 ${\beta}-endorphin$은 그의 진통효과에 있어서 보인 상승작용은 척수상부에 위치하고 있는 opioid deptide의 양이 변해서가 아니라 다른 기전에 의하여 조절되어지고 있음을 시사한다.

• Archives of Pharmacal Research
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• 제25권6호
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• pp.865-872
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• 2002

In the course of the investigations of natural antioxidants, we examined the antioxidant activities of the methanol (MeOH) extracts of some selected Prunus species, including P. buergeriana, P. davidiana, P padus, P. pendula for. ascendens, P. sargentii, P. serrulata var. spontanea and P. yedoensis by three methods as represented by the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, total ROS (reactive oxygen species) and the peroxynitrite ($ONOO^-$) scavenging activity tests. We also evaluated the activities of the organic solvent-soluble fractions, including the dichloromethane ($CH_2Cl_2$), ethyl acetate (EtOAc), n-butanol (n-BuOH) fractions and the water ($H_2O$) layer of P. serrulata var. spontanea leaves. By means of bioassay-directed fractionation, we isolated eleven known flavonoids (1-11) from the EtOAc soluble fraction of the MeOH extract of the Prunus serrulata var. spontanea leaves, exhibiting strong antioxidant activity and characterized as prunetin (1), genistein (2), quercetin (3), prunetin $4'-O-{\beta}-glucopyranoside$ (4), kaempferol $3-O-{\alpha}-arabinofuranoside$ (5), prunetin $5-O-{\beta}-glucopyranoside$ (6), kaempferol $3-O-{\beta}-xylopyranoside$ (7), genistin (8), kaempferol $3-O-{\beta}-glucopyranoside$ (9), quercetin $3-O-{\beta}-glucopyranoside$ (10) and kaempferol $3-O-{\beta}-xylopyranosyl-(1{\rightarrow}2)-{\beta}-glucopyranoside$ (11). Compounds 3 and 10 showed good activities in all tested model systems. Compounds 2 and 8 showed scavenging activities in the DPPH and $ONOO^-$ tests, while compounds 5, 7, 9 and 11 were active in the $ONOO^-$ and ROS tests. On the other hand, compounds 1, 4 and 6 did not show any activities in the tested model systems.