• 대한한방부인과학회지
• /
• 제18권4호
• /
• pp.54-71
• /
• 2005

Purpose : The purpose of this research was to investigate the effects of Saurui Herba Seu Rhizoma(SHSR) on Anti-inflammatory properties in Raw264.7 cell line and murine models of inflammation. Methods : To investigate the effects of Saurui Herba Seu Rhizoma(SHSR) on anti-inflammation, we study cytotoxicity effects of SHSR on Mouse Lung Fibroblast Cells and Peritoneal Macrophages, Inhibitory effects of SHSR on the nitric oxide (NO) release, the ROS production, and the interleukin-6 production. Results : The cytotoxicity of SHSR on mouse lung fibroblast Cells and Raw264.7 cell line was not observed. SHSR in RAW264.7 cell line inhibited $IL-1{\beta}$, IL-6 mRNA gene expression depending upon the concentrations of extract and inhibited IL-18 mRNA gene expression at 100 ${\mu}g/ml$ of extract. SHSR in RAW264.7 cell line inhibit COX-2 mRNA gene expression at 100, 10 ${\mu}g/ml$ of extract. SHSR in RAW264.7 cell line inhibited NOS-II mRNA gene expression depending upon the concentrations of extract. SHSR in RAW264.7 cell line didn't inhibit $TNF-{\alpha}$ mRNA　gene expression. SHSR in RAW264.7 cell line decreased IL-6 production depending　upon the concentrations of extract. SHSR in RAW264.7 cell line decreased $ITNF-{\alpha}$ production according to the concentrations of extract. SHSR in RAW264.7 cell line inhibited NO release specially SHSR 100, 10 ${\mu}g/ml$ concentrations of extract. SHSR inhibit ROS production depending upon the concentrations of extract. Conclusion : These results suggest that SHSR can be used treating a lot of women disease caused by inflammation.

• 한국식품과학회지
• /
• 제29권3호
• /
• pp.539-545
• /
• 1997

본 연구에서는 대두요구르트의 올리고당을 증가시키기 위해 장엽콩 및 진품콩으로 제조한 두유에 lactose를 첨가한 후 L. bulgaricus와 K. lactic를 혼합배양 하여 L. bulgaricus로 단독배양한 시료들과 비교하였다. Lactose를 첨가하지 않은 시료는 젖산발효에 의해 stachyose, raffinose, sucrose, glucose의 양이 모두 감소하였으나, lactose를 첨가하여 단독배양한 시료는 올리고당의 감소량이 lactose를 첨가하지 않은 시료보다 더 적었다. Lactose를 첨가한 장엽시료에서, 배양방법과 lactose 첨가농도별 stachyose와 raffinose의 증가량을 보면, 단독배양은 장엽두유의 양과 같거나 감소하였던 반면 혼합배양의 경우는 lactose를 2% 첨가하여 24시간 배양한 시료는 125.0%, 36시간 배양한 시료는 127.0% 증가하였으며 lactose를 4% 첨가하여 24시간 배양한 시료는 112.5%, 36시간 배양한 시료는 120%가 증가하였으므로 lactose를 2% 첨가한 장엽시료에서 올리고당의 생성량이 더 많았다. Lactose를 첨가한 진품시료에 있어서도 단독배양의 경우 진품두유보다 올리고당이 감소하였던 반면, 혼합배양의 경우는 lactose를 2% 첨가하여 24시간 배양한 시료는 118.0%, 36시간 배양한 시료는 141.0% 증가하였고 lactose 4%를 첨가하여 24시간 배양한 시료는 123.0%, 36시간 배양한 시료는 135.9% 증가하였으므로, 36시간 혼합 배양한 진품시료의 경우 lactose 2% 첨가한 시료에서 올리고당의 생성량이 더 많았다. 한편 대두품종별 혼합배양에 의한 올리고당 생성량을 보면 36시간 혼합 배양시 진품시료에서 장엽시료에서 보다 올리고당의 생성량이 더 많았다. 이상의 결과로 볼 때, lactose를 첨가한 장엽 및 진품 대두요구르트의 발효과정에서 lactose가 분해되어 생성된 galactose는 효모(K. lactis)의 당전이력에 의해 sucrose와 결합하여 올리고당을 생성하였음을 알 수 있다.

• Journal of Ginseng Research
• /
• 제11권2호
• /
• pp.145-252
• /
• 1987

Isolated rat adipocytes are well known to possess opposite pathways of lipid metabolism: lipolysis and ipogenesis. Both of the metabolism respond to various biologically active substances such as epinephrine, ACTH and insulin. Epinephrine and ACTH stimulate lipolysis and insulin accelerates lipogenesis. Recently, Korean red ginseng powder was found to contain adenosine and an acidic poptide which inhibited epinephrine-induced lipolysis and sl imulated insulin-mediated lipogenesis from added glucose. The acidic peptide is consisted mainly of glutamic acid and glucose. Ginsenosides Rb1 and Re inhibited ACTH-induced lipolysis in isolated rat adipocytes, while they did not affect insulinstimulated lipogenesis, Thus, all these substances extracted from Korean red ginseng exhibited selective modulations toward the opposite metabolic pathways in rat adipocyte; They inhibited the lipolysis but not the lipogenesis. We call these substances"selective modulators". Recently, we isolated a toxic substance named "toxohormone-L " from ascites fluid of patients with various malignant tumors. The toxohormone-L stimulated lipolysis in rat adipocytes and induced anorexia in rats. Both the lipolytic and the anorexigenic actions of toxohormone-L were found to be inhibited by ginsenoside Rb2 in Korean red ginseng. Based on these results, physiological signifi¬cances of these substances in Korean red ginseng were discussed. Pan ax ginseng is a medicinal plant long used in treatment of various pathological states including general complaints such as head ache, shoulder ache, chilly constitution and anorexia in cancer patients, There have been many pharmacological studies on Panax ginseng roots. Petkovllreported that oral administration of an aqueous alcoholic extract of ginseng roots decreased the blood sugar levtl of rabbits. Saito2lreported that Panax ginseng suppressed hyperglycemia induced by epinephrine and high carbohydrate diets. These findings suggest that Panax ginseng roots contain insulin-like substances. Previously, we demonstrated that gin¬seng roots contain an insulin-like peptide which inhibits epinephrine-induced lipolysis and stimulated insulin-mediated lipogenesis. In 1984, we suggested that such an insulin-like substance should be called a selective modulator4). Present investigation describes the details of the selective modulators in ginseng roots. During progressive weight loss in patients with various neoplastic disease, depletion of fat stores have been observed. The depletion of body fat during growth of neoplasms is associated with increase in plasma free fatty acids. Recently, we found that the ascites fluid from patients with hepatoma or ovarian tumor and the pleural fluid from patients with malignant lymphoma elicited fatty acid release in slices of rat adipose tissue in vitro. The lipolytic factor, named"toxohormone-L". was purifed from the ascites fluid of patients with hepatoma. The isolated preparation gave a single band on both disc gel electrophoresis and sodium dodecyl sulfate(SDS)-acrylamide gel electrophoresis in the presence of ${\beta}$-mercaptoethanol. Its molecular weight was determined to be 70,000-75,000 and 65,000 by SDS-acrylamide gel electrophoresis and analytical ultracentrifugation, respectively. Injection of toxohormone-L into the lateral ventricle of rats significantly suppressed food and water intakes. There was at least 5 hr delay between its injection and appearance of its suppressive effect. In the present study, we also tried to find a inhibitory substance toward toxohormone-L from root powder of ginseng.

• Journal of Ginseng Research
• /
• 제36권2호
• /
• pp.135-145
• /
• 2012

Previously, we reported that Helicobacter pylori-associated gastritis and gastric cancer are closely associated with increased levels of hydrogen sulfide ($H_2S$) and that Korean red ginseng significantly reduced the severity of H. pylori-associated gastric diseases by attenuating $H_2S$ generation. Because the incubation of endothelial cells with $H_2S$ has been known to enhance their angiogenic activities, we hypothesized that the amelioration of $H_2S$-induced gastric inflammation or angiogenesis in human umbilical vascular endothelial cells (HUVECs) might explain the preventive effect of Korean red ginseng on H. pylori-associated carcinogenesis. The expression of inflammatory mediators, angiogenic growth factors, and angiogenic activities in the absence or presence of Korean red ginseng extracts (KRGE) were evaluated in HUVECs stimulated with the $H_2S$ generator sodium hydrogen sulfide (NaHS). KRGE efficiently decreased the expression of cystathionine ${\beta}$-synthase and cystathionine ${\gamma}$-lyase, enzymes that are essential for $H_2S$ synthesis. Concomitantly, a significant decrease in the expression of inflammatory mediators, including cyclooxygenase-2 and inducible nitric oxide synthase, and several angiogenic factors, including interleukin (IL)-8, hypoxia inducible factor-1a, vascular endothelial growth factor, IL-6, and matrix metalloproteinases, was observed; all of these factors are normally induced after NaHS. An in vitro angiogenesis assay demonstrated that NaHS significantly increased tube formation in endothelial cells, whereas KRGE pretreatment significantly attenuated tube formation. NaHS activated p38 and Akt, increasing the expression of angiogenic factors and the proliferation of HUVECs, whereas KRGE effectively abrogated this $H_2S$-activated angiogenesis and the increase in inflammatory mediators in vascular endothelial cells. In conclusion, KRGE was able to mitigate $H_2S$-induced angiogenesis, implying that antagonistic action against $H_2S$-induced angiogenesis may be the mechanism underlying the gastric cancer preventive effects of KRGE in H. pylori infection.

• Journal of Ginseng Research
• /
• 제38권3호
• /
• pp.167-172
• /
• 2014

Background: Roles of immune reaction and toll-like receptor-4 (TLR-4) have widely been established in the pathogenesis of alcoholic liver disease (ALD). Methods: We evaluated the biologic efficacy of Korean Red Ginseng (KRG), urushiol, and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) in mouse models of ALD. Sixty C57BL/6 mice were equally divided into six feeding groups for 10 weeks: normal diet, alcohol, control, alcohol + KRG, alcohol + urushiol, and alcohol + probiotics. Alcohol was administered via a LiebereDeCarli liquid diet containing 10% alcohol. TLR-4 expression, proinflammatory cytokines, and histology, as well as the results of liver function tests were evaluated and compared. Results: No between-group differences were observed with regard to liver function. TLR-4 levels were significantly lower in the KRG, urushiol, and probiotics groups than in the alcohol group ($0.37{\pm}0.06ng/mL$, $0.39{\pm}0.12ng/mL$, and $0.33{\pm}0.07ng/mL$, respectively, vs. $0.88{\pm}0.31ng/mL$; p < 0.05). Interleukin-$1{\beta}$ levels in liver tissues were decreased among the probiotics and KRG groups. The tumor necrosis factor-${\alpha}$ level of liver tissue was decreased in the KRG group. Conclusion: The pathological findings showed that alcohol-induced steatosis was significantly reduced by KRG and urushiol. As these agents improve immunologic capacity, they may be considered in potential anti-ALD treatments.

• 동아시아식생활학회지
• /
• 제19권4호
• /
• pp.570-578
• /
• 2009

채취시기가 다른 6종의 녹차를 시료로 택하여 실제 음용 조건으로 추출하여 일반 성분, 카페인 및 총 페놀 함량을 분석하였고, 전자공여능에 의한 항산화 활성, ACE 저해도, SOD 유사 활성, Tyrosinase 저해율, 아질산염 소거능 및 항균 활성을 조사하였다. 그 결과 일반 성분과 카페인의 경우 채취시기가 빠른 것일수록 총질소 및 카페인 함량이 높게 나타났다. 반면, 총페놀, 전자공여능, ACE 저해율, SOD 유사활성은 아질산염 소거능의 경우 채취시기에 따라서 크게 차이를 나타내지 않았으나, Tyrosinase 저해율의 경우 채취시기가 늦어질수록 약간 증가하는 경향을 나타내었다. 항균 활성의 경우 일로를 제외한 5종의 시료의 경우 항균성을 나타내는 것을 확인하였다.

• Bulletin of the Korean Chemical Society
• /
• 제30권5호
• /
• pp.1121-1126
• /
• 2009

This paper describes a pattern recognition method of Magnoliae flos based on a gas chromatographic/mass spectrometric (GC/MS) analysis of the essential oil components. The botanical drug is mainly comprised of the four magnolia species (M. denudata, M. biondii, M. kobus, and M. liliflora) in Korea, although some other species are also being dealt with the drug. The GC/MS separation of the volatile components, which was extracted by the simultaneous distillation and extraction (SDE), was performed on a carbowax column (supelcowax 10; 30 m{\time}0.25 mm{\time}0.25{\mu}m$) using temperature programming. Variance in the retention times for all peaks of interests was within RSD 2% for repeated analyses (n = 9). Of the 74 essential oil components identified from the magnolia species, approximately 10 major components, which is $\alpha$-pinene, $\beta$-pinene, sabinene, myrcene, d-limonene, eucarlyptol (1,8-cineol), $\gamma$-terpinene, p-cymene, linalool, $\alpha$-terpineol, were commonly present in the four species. For statistical analysis, the original dataset was reduced to the 13 variables by Fisher criterion and factor analysis (FA). The essential oil patterns were processed by means of the multivariate statistical analysis including hierarchical cluster analysis (HCA), principal component analysis (PCA) and discriminant analysis (DA). All samples were divided into four groups with three principal components by PCA and according to the plant origins by HCA. Thirty-three samples (23 training sets and 10 test samples to be assessed) were correctly classified into the four groups predicted by PCA. This method would provide a practical strategy for assessing the authenticity or quality of the well-known herbal drug, Magnoliae flos.

• 한국임상약학회지
• /
• 제8권2호
• /
• pp.83-88
• /
• 1998

Improved knowledge of the time course of antimicrobial activity should provide useful information for designing optimal dosage regimen. In contrast to $\beta$-lactam, aminoglycosides tend to induce a prolonged postantibiotic effect against aerobic or facultative gram negative rods and clinical outcome was significantly correlated with achieving peak serum concentrations. The objective of this study was to compare the efficacy, safety of e same total daily dose of amikacin and gentamicin given either twice or thrice daily in the treatment of patients. Consecutive patients over 20 years old with a suspected or confirmed infection for which an aminoglycosides was indicated were eligible. Exclusion criteria were known allergy to aminoglycosides, renal impairment, granulocytopenia and pregnancy. Patients were treated with intravenous amikacin 15 mg/kg/day or gentamicin 4.5 mg/kg/day either in two devided or in three devided. Seventy-four patients with infection were enrolled in this study of amikacin twice daily (A2, n=29), gentamicin twice daily (G2, n=8) vs amikacin thrice daily (A3, n=30), gentamicin thrice daily (G3, n=7). Baseline characteristics were comparable in G2 and G3. The clinical cure rate (including partial improve) were $89.0\%\;and\;86.0\%$ in A2 group and A3 group respectively. The bacteriologic cure rate were $99.0\%\;and\;85.7\%$ in A2 group and in A3 group respectively. The clinical and bacteriologic effects were difficult to compare G2 with G3, because of the small numbers of patients. The serum creatinin rose in $3.44\%$ (1 in 29) of patients in the A2 group compared to $13.3\%$ (4 in 30) in e A3 group. Although audiometry was not performed, there was no clinical evidence of ototoxicity in any of the patients. In our opinion, twice-daily regimen of aminoglycosides is more effective and less nephrotoxic than thrice-daily regimen.

• 한국임상약학회지
• /
• 제21권4호
• /
• pp.297-304
• /
• 2011

배경: 골다공증은 골대사의 불균형으로 인해 골 흡수가 골 형성보다 많아져 골밀도가 감소함으로서 발생한다. 골다공증의 이상적인 치료목표는 골형성을 증가시키거나 골소실을 방지하여 골량을 현 상태로 유지하는 것이다. 따라서 향후 발생되는 골소실을 에방하는 것이 골다공증의 원칙적이고 효과적인 치료방법이 될 것이다. 본 연구에서는 흑효모 중 $Aureobasidium$ $pullulans$으로부터 유래한 폴리칸(베타-글루칸)이 중년여성의 골대사에 미치는 영향을 규명하고자 하였다. 연구방법: 골대사에 대한 폴리칸의 효과를 규명하기 위해 12주간의 무작위배정, 이중눈가림, 플라세보 대조 임상연구를 수행하였다. 총 60명(폴리칸 투여군30명, 플라세보 투여군 30명)의 중년 여성 피험자가 등록되어 이 중 총 58명의 피험자가 최종적으로 12주간의 임상연구를 종료하였다. 결 과: 폴리칸(150 mg/d) 투여 12주 후, 폴리칸 투여군은 요 중 Deoxypyridinoline (DPD) 농도가 유의적인 감소를 보였다($P$=0.014). 혈청 중 Osteocalcin(OSC) 농도는 두 군 모두에서 유의적으로 증가하였으며, bone-specific alkaline posphatase (bALP) 와 collagen type 1 cross-linked C-telopeptide (CTx)는 유의적 변화가 보이지 않았다. 폴리칸은 골밀도(BMD)와 혈청 부갑상선 호르몬(iPTH)에 대해 유의적인 변화를 보이지 않았으나, 24시간 요 중 Ca 배설량은 폴리칸 투여군에서 유의하게 감소되었다($P$=0.028). 또한 폴리칸 투여군에서 고밀도지단백 콜레스테롤(HDL-cholesterol) 농도의 증가 경향 및 중성지방(triglyceride)의 유의적인 감소가 보였다. 임상연구 기간 중에 발생한 이상반응은 두 군간에 유의적인 차이를 보이지 않았다. 결 론: 본 연구에서는 폴리칸이 골대사 및 지질에 대해 일부 개선효과가 있음을 보여주었다. 그러나, 골다공증 예방 측면에서 보다 장기적인 임상연구와 피험자 수를 확대하여 골대사 및 지질대사에 대한 폴리칸의 예방적 효과를 규명할 필요가 있을것으로 사료된다.

• 대한치과기공학회지
• /
• 제32권2호
• /
• pp.65-74
• /
• 2010

Purpose : To understand the impact on the strength or restoration force by the change of heating temperature when soldering 18-8 stainless steel round wire which is the chrome-nickel class for dental orthodontic device production. Methods : The following conclusions were made upon the results from tensile strength test, 3 point bending test, and $90^{\circ}$ bending fatigue test with 24 samples that had been applied with condition 1 (before heat treatment - natural) and condition 2 (after heat treatment - mooring 30 seconds after heating up to $500^{\circ}C$, $700^{\circ}C$, and $900^{\circ}C$) to ${\phi}0.4mm$, ${\phi}0.7{\beta}mm$, 18-8 stainless steel round wire (spring hard) by Jinsung Company. Results : When it was heat-treated at $900^{\circ}C$, both ${\phi}0.4mm$ and ${\phi}0.7mm$ showed very low tensile strengths compared to the heat treated cases at $500^{\circ}C$ and $700^{\circ}C$ Yield strengths of both ${\phi}0.4mm$ and ${\phi}0.7mm$ showed very low compared to the heat treated cases at natural, $500^{\circ}C$, and $700^{\circ}C$, as well. Upon the results of 3 point bending test, the heat treated case at $900^{\circ}C$ showed very low in both ${\phi}0.4mm$ and ${\phi}0.7mm$, compared to the heat treated cases at natural, $500^{\circ}C$, and $700^{\circ}C$. Tensile strength of both ${\phi}0.4mm$ and ${\phi}0.7mm$ as well, showed very low compared to the heat treated cases at natural, $500^{\circ}C$, and $700^{\circ}C$. Upon the results of $90^{\circ}$ bending fatigue test, the heat treated case at $900^{\circ}C$ showed the highest wave node resistance in both ${\phi}0.4mm$ and ${\phi}0.7mm$. Conclusion : This study concluded that heating temperature change during wire soldering impacts on the characteristics of orthodntic wire.