• 제목/요약/키워드: ${\alpha}-Factor$

검색결과 4,705건 처리시간 0.028초

사료내 벤조산 첨가가 이유돼지의 장내 미생물 균총 및 면역체계에 미치는 영향 (Effect of Dietary Benzoic Acid on Beneficial Microflora and Immune Response in the Intestine of Weaning Pigs)

  • 오희경;최영환;김영해;김유용
    • 생명과학회지
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    • 제22권10호
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    • pp.1307-1315
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    • 2012
  • 본 연구에서는 이유자돈 사료에 벤조산 첨가가 위장 내 소화물과 방광 내 뇨의 pH, 장내 유익 미생물 균총수 및 장 조직 내 염증성 싸이토카인 유전자 발현양에 미치는 영향을 조사하였다. 자돈기 5주 동안 이유자돈 사료 내 벤조산 0.3%과 0.5% 첨가는 자돈 위 내 pH는 감소시키지는 않았으나, 방광 내 뇨의 pH 수치는 유의적으로 감소하는 것으로 나타났다. Lactobacillus casei 수는 맹장부위에서 벤조산 0.5% 첨가구와 대조구에서 유의적으로 가장 높게 나타났다. Lactobacillus plantarum 수는 맹장부위에서 벤조산 0.5% 첨가구에서 가장 높게 나타났다. 또한, 직장부위에서 L.plantarum 수는 대조구를 제외하고 모든 처리구에서 유의적으로 높게 나타났다. Bacillus subtillis 수는 맹장부위에서 벤조산0.5% 첨가구와 대조구에서 유의적으로 가장 높게 나타났으며, 직장부위에서는 벤조산0.3%와 0.5% 첨가구에서 가장 높게 나타났다. IL-$1{\beta}$ mRNA 유전자 발현 수준은 소장 상단에서는 벤조산 0.5%와 항생제 처리구에서, 소장중부에서는 벤조산 0.3%와 0.5% 처리구에서, 말단부위에서는 벤조산 0.3% 처리구에서 대조구보다 유의적으로 낮게 나타났다. IL-6 mRNA 유전자 발현 수준은 소장중부에서는 벤조산 0.5% 처리구에서 대조구보다 유의적으로 낮게 나타났다. TNF-${\alpha}$ mRNA 유전자 발현 수준은 소장 중단에서 벤조산 0.5%와 항생제 처리구에서 대조구 보다 낮게 나타났다. 본 실험을 통하여, 자돈 사료 내 benzoic acid 0.5% 첨가는 뇨의 pH 감소와 장내 유익 미생물 균총 수 조절 및 소장 내 면역체계 반응에 긍정적인 영향을 미치므로 자돈 건강을 향상 할 수 있으리라 사료된다.

모링가 뿌리 추출물에 대한 신장섬유화 억제 효과 (Anti-Fibrotic Effects by Moringa Root Extract in Rat Kidney Fibroblast)

  • 박수현;장영채
    • 생명과학회지
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    • 제22권10호
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    • pp.1371-1377
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    • 2012
  • 신장섬유화는 내 외부적인 요인들에 의해 발생하며, 그 요인들에 의해 염증이 생기고 지속적인 손상이 일어날 경우 신기능의 상실이 유발된다. 또한 신장섬유화는 세포 외 기질의 과다축적, TGF-${\beta}$나, TNF-${\alpha}$, IL-1과 같은 사이 토카인에 의해 발생하며, TGF-${\beta}$는 신장 섬유화의 과정과 Type I collagen과 fibronectin, PAI-1을 포함한 섬유화 관련 인자들의 발현 유도에 중요한 역할을 한다. 본 연구에서는 TGF-${\beta}$를 처리한 신장섬유화 유도 모델에서 Moringa oleifera Lam 추출물에 대한 섬유화 관련 인자들의 영향을 확인하였다. 실험 결과 TGF-${\beta}$로 유도된 신장 섬유화 세포에서 모링가 추출물이 fibronectin, Type I collagen과 PAI-1의 단백질 및 mRNA 발현을 저해하였으며, 모링가 추출물 중 모링가 뿌리추출물이 가장 영향이 있는 것으로 확인 되었다. 모링가 뿌리추출물이 어떠한 기전을 통하여 섬유화 관련 인자들의 발현을 조절하는지 알아보기 위한 TGF-${\beta}$로 유도된 $T{\beta}RII$ 및 그 하위 기전의 인산화 정도를 확인한 실험에서 모링가 뿌리추출물이 TGF-${\beta}$로 유도된 $T{\beta}RII$과 그 하위기전의 Smad4, ERK의 인산화를 저해하였다. 그러나 TGF-${\beta}$에 의해 유도된 JNK와 p38, PI3K/AKT의 인산화에는 영향이 없었다. 따라서 모링가 뿌리추출물이 TGF-${\beta}$로 유도된 신장 섬유아세포에서 $T{\beta}RII$와 그 하위 기전인 Smad4, ERK를 통해서 Type I collagen 과 fibronectin, PAI-1의 발현을 조절하여 섬유화를 저해 한다는 것을 예상할 수 있다. 결론적으로 모링가 뿌리추출물이 섬유화 치료 및 완화에 좋은 물질이 될 수 있을 것으로 생각된다.

마쇄처리 고추의 냉동저장중 이화학적 성분의 변화 (The Physico-Chemical Changes of the Mashed Red Pepper During Frozen Storage)

  • 설민숙;이현자;박소희;김종군;황성연
    • 한국식생활문화학회지
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    • 제19권2호
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    • pp.209-216
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    • 2004
  • 마쇄처리 고추를 냉동 저장하면서 그 이화학적 변화 및 이용 방법을 연구하기 위하여 포청천을 태양건조후 고춧가루로 만든 것과 마쇄한 다음 브렌칭, 비타민 C 첨가, NaCl 첨가, aging 처리 등을 하고 냉동저장하면서 품질변화를 비교하였다. L, a, b값 가운데 적색도를 나타내는 a값이 포청천의 경우 기존 고춧가루의 31.51에 비하여 마쇄처리 고추는 모두 $33.53{\sim}33.84$로 마쇄처리한 것이 적색도가 좋았다. Capsanthin 함량은 마쇄 냉동저장시 비타민 C 첨가구가 1.8% 감소하여 비타민 C 첨가가 capsanthin 함량유지에 도움이 됨을 알 수 있었다. 매운맛 성분인 capsaicin과 dihydrocapsaicin은 냉동저장중 고춧가루의 경우 11.9%, 18.32% 감소하여 dihydrocapsaicin이 capsaicin 보다 빠르게 감소되었으며 전반적으로 마쇄 냉동저장한 것이 고춧가루보다 신미성분의 감소가 적었다. 마쇄처리 고추에서는 비타민 C를 첨가한 것이 capsaicin과 dihydrocapsaicin의 감소가 가장 적게 나타났다. 비타민 C는 마쇄고추를 브렌칭할 때 잔존량이 12.0 mg/100 g으로 마쇄고추의 44.0 mg/100 g에 비하여 파괴가 가장 많이 일어났으며 NaCl 첨가구의 경우도 13.2 mg/100 g으로 비슷하였다. 그러나 비타민 C를 첨가한 경우는 52.0 mg/100 g이 잔존하였고 냉동저장시 마쇄고추의 69.0%에 비하여 32.9%가 감소하여 비타민 C 첨가가 고추에 함유된 비타민 C 유지에 도움이 되었다.

청소년의 기질과 스트레스 대처전략이 심리적 안녕감에 미치는 영향 (Effects of Temperament and Stress Coping Strategies on Psychological Well-Being of Adolescents)

  • 이상미;임정하
    • 한국가정과교육학회지
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    • 제27권2호
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    • pp.121-135
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    • 2015
  • 본 연구에서는 청소년의 기질과 스트레스 대처전략이 심리적 안녕감에 미치는 영향력을 알아보고자 하였다. 연구대상은 서울에 소재한 2개 중학교와 2개 고등학교에 재학 중인 남학생 216명, 여학생 183명, 전체 399명이었다. 첫째, 성별과 학교급별에 따른 청소년의 심리적 안녕감은 유의한 차이가 있었다. 둘째, 청소년의 기질은 심리적 안녕감과 유의한 상관을 보였는데, 청소년이 활동적, 지속적인 기질일수록, 경직성이 낮을수록 심리적 안녕감은 높게 나타났다. 다음으로 스트레스 대처전략은 심리적 안녕감과 유의한 상관을 보였는데, 청소년들이 문제중심 대처전략의 사용이 많을수록, 정서분출 대처전략과 정서조절 대처전략의 사용이 적을수록 심리적 안녕감은 높았다. 셋째, 청소년의 성별, 학교급별, 기질과 스트레스 대처전략이 심리적 안녕감에 미치는 영향력을 살펴본 결과, 가장 큰 영향력을 미치는 요인은 문제중심 대처전략이고, 그 다음으로는 활동성, 정서분출 대처전략, 지속성, 성별 순으로 영향을 미쳤다. 특히, 청소년의 스트레스 대처전략은 성별, 학교급별, 기질을 통제한 뒤에도 심리적 안녕감을 의미있게 설명하였다. 이러한 연구결과는 스트레스 대처전략의 올바른 사용을 향상시켜나가기 위한 교육이 필요함을 제시해주었고, 특히 청소년기의 성공적인 적응을 위해 문제중심 대처전략의 사용은 청소년이 행복해질 수 있는 실제적 방안이 될 수 있음을 시사한다.

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기독병원과 일반병원의 조직문화 특성에 따른 조직성과 분석 (Analysis on Organization Performance Based on Hospital Culture)

  • 김운신;남은우
    • 한국병원경영학회지
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    • 제4권2호
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    • pp.242-265
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    • 1999
  • Our study attempt is to see organizational performance according to the differences between types of hospital cultures. To determine theoretical relationship between the organizational culture and the performance, we select two hospitals in Pusan which are different in the purposes and shapes of establishment. We surveyed their members on a questionnaire based on the type of the organizational culture of the two institutions, analyzed, and review its organizational effectiveness. For the survey with questionnaires, which had been preliminary studied to raise its validity, question forms were distributed to 528 persons in April, 1999 based on the self-responses and recollected within 48 hours. The recollection rate was high(89.96%) and the quantity of questionnaires used for our final analysis was 430(81.44%). The Cronbach Coefficient Alpha of the questionnaires was 0.742. Regarding statistical techniques for analysis of the written materials, dispersion analysis(ANOVA) was adapted to test the organizational effectiveness of the two hospitals having the different organizational cultures, and Pearson Correlation was applied to determine correlations was among all variables. T-test was performed to test organizational effectiveness based on the differences in the extent of sharing the culture, organizational committment and work satisfaction between the two health institutions. From our analysis, we obtain the following conclusions. First, concerning with organizational culture of the two hospital, one of which is a christian hospital and the other is a private foundation hospital, the former is conservative and human-oriented but the latter focuses on renovation and accomplishment. Second, the private establishment has a relatively higher organizational effectiveness that the religious hospital as a result of analyzing the extent of sharing culture, organizational committment and work satisfaction. Third, it has been found that the correlations between the extent of the sharing culture and the organizational committment, the extent and work satisfaction, and the committment and the satisfaction are respectively positive influencing organizational effectiveness, especially work satisfaction. Fourth, cultural factors by which the christian hospital is affected more positively including human relations among its members, belief, its idea of establishment, tradition, work responsibility, power, and wage. On the other hand, factors such as director's leadership, personnel management, wage, hospital regulations and department managers' management ability have been seen as negative influences in order. And fifth, for the private foundation hospital human relations among its members, wages, work responsibility, director's leadership and department managers' management ability were positive in their sequence while wages, personnel management, hospital regulations, welfare and department managers' management ability were considered as negative influences in order. As these results of this study, the higher extent of sharing organizational culture, the more increasing in both organization committment and work satisfaction, the higher the effectiveness. Although it was somewhat difficult to generalize the results whose subjects were the two hospitals only, it was obvious that organizational culture was an important influential factor of organizational effectiveness. It is questionable that the extent of sharing organizational culture, organizational committment and work satisfaction as variables affecting the effectiveness have their validity, but this study has its significance in that it provided an approaching to evaluate the organizational culture of individual hospitals making allowances for such variables related to the general activities in its hospital. We hope the results of the study could be useful for the managerial strategies of the institutions.

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맞벌이부부의 배우자 지지, 직장 지지 및 일-가족 전이가 일-생활 균형에 미치는 자기효과와 상대방효과 (The influences of spousal support and work-family spillover on work-life balance in dual-earner couples with children: Testing actor and partner effects)

  • 정서린;장윤옥
    • 가족자원경영과 정책
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    • 제21권3호
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    • pp.97-119
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    • 2017
  • The purpose of this study was to find out the actor effect and the partner effect in relation to spousal support, job support, work-family spillover, and work-life balance in dual-earner couples. The subjects of this study were 176 dual-earner couple, whose youngest children are under the age of 16 and who work over 15 hours each week. The research tool was a questionnaires. Factor analysis, Cronbach's ${\alpha}$, correlation analysis, and APIM were performed for the data analysis. The main results of this study as follows. First, for both wives and husbands, perceived spousal support had a positive actor effect on work-life balance. However, perceived husband's spouse support for wife's support had positive partner effect on the wife's work-life balance, the wife's perceived spousal support for her husband' did not have a partner actor effect on the husband's work-life balance. Second, for both wives and husbands, job support had a positive actor effect on work-life balance. In addition, the wife's job support had a positive partner effect on the husband's work-life balance, however, the husband's job support did not have a partner actor effect on the wife's work-life balance. Third, for both wives and husbands, work-family conflict had a negative actor effect on work-life balance. In addition, the wife's work-family conflict had a negative partner effect on the husband's work-life balance, however, the husband's work-family conflict did not have a partner actor effect on the wife's work-life balance. Fourth, for both wives and husbands, work-family enrichment had a positive actor effect on work-life balance. In addition, the husband's work-family enrichment had a positive partner effect on the wife's work-life balance, however, the wife's work-family enrichment did not have a partner actor effect on the husband's work-life balance.

Synergic Effects of Mixed Formula Consisted of Polycan and Calcium-gluconate on the Experimental Periodontitis and Alveolar Bone Loss in Rats

  • ;;;이영준;구세광
    • 대한예방한의학회지
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    • 제18권1호
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    • pp.125-138
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    • 2014
  • Objective : Polycan, exopolymers purified from Aureobasidium pullulans SM-2001 and calcium gluconate have been showed favorable inhibitory effects on the periodontitis and related alveolar bone losses through antioxidant and anti-inflammatory activities, respectively. In the present study, we intended to observe the possible synergic effects of mixed formula consisted of Polycan and calcium gluconate on ligation-induced experimental periodontitis and related alveolar bone losses in rats, and to select the fittest compositions for further developing as effective agents to ameliorate periodontal diseases. Method : Experiments were conducted as two separated two tests - first is synergic effects of Polycan and calcium gluconate 1:1, 1:9 and 9:1 mixtures, and second is 1:99, 2:98, 4:96, 8:92 and 1:9 mixtures. Experimental periodontal diseases were induced by ligature placed around the cervix of upper left incisior teeth of rats. One day after ligation placements, 200mg/kg of each single or mixed formulas of Polycan or/and calcium gluconate were orally administered for 10 days. The changes on the alveolar bone loss index and maxillary bone mineral density (BMD) were observed for detecting alveolar bone losses, and for anti-inflammatory effects, myeloperoxidase (MPO) activities and proinflammatory cytokine (tumor necrosis factor; TNF-${\alpha}$) contents were also evaluated in gingival tissues around ligature placed incisior teeth. The results of mixtures were compared with those of singe Polycan and calcium gluconate treated rat. Results : Each single or mixed formulas of Polycan or/and calcium gluconate favorably and significantly inhibited the inflammatory changes. The inhibitory effects of mixed formula consisted of Polycan and calcium gluconate 1:9 showed against periodontitis and related alveolar bone losses as compared with those of each Polycan and calcium gluconate single formula (p<0.05). In second experiment, Polycan and calcium gluconate 2:98, 4:96, 8:92 and 1:9 mixed formulas also showed significant increased anti-inflammatory and inhibitory effects against alveolar bone losses as compared with those of each single formula. Among them, Polycan and calcium gluconate 2:98 showed the highest efficacy against to ligation-induced experimental periodontitis and related alveolar bone losses. Conclusion : The results obtained in this study suggest that appropriated mixtures of Polycan and calcium gluconate showed synergic inhibitory effects against ligation-induced experimental periodontitis and related alveolar bone losses in rats. Moreover, Polycan and calcium gluconate 2:98 showed the highest efficacies in this experiment, suggesting the fittest composition for further developing as effective agents to ameliorate periodontal diseases.

hPDX1 유전자의 삽입에 의한 직접 췌도세포 분화 (Transdifferentiation of α-1,3-Galactosyltransferase Knock Out (GalT KO) Pig Derived Bone Marrow Mesenchymal Stromal Cells (BM-MSCs) into Pancreatic Cells by Transfection of hPDX1)

  • 옥선아;오건봉;황성수;김영임;권대진;임기순
    • 한국수정란이식학회지
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    • 제30권3호
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    • pp.249-255
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    • 2015
  • Diabetes mellitus, the most common metabolic disorder, is divided into two types: type 1 and type 2. The essential treatment of type 1 diabetes, caused by immune-mediated destruction of ${\beta}-cells$, is transplantation of the pancreas; however, this treatment is limited by issues such as the lack of donors for islet transplantation and immune rejection. As an alternative approach, stem cell therapy has been used as a new tool. The present study revealed that bone marrowderived mesenchymal stromal cells (BM-MSCs) could be transdifferentiated into pancreatic cells by the insertion of a key gene for embryonic development of the pancreas, the pancreatic and duodenal homeobox factor 1 (PDX1). To avoid immune rejection associated with xenotransplantation and to develop a new cell-based treatment, BM-MSCs from ${\alpha}$-1,3-galactosyltransferase knockout (GalT KO) pigs were used as the source of the cells. Transfection of the EGFP-hPDX1 gene into GalT KO pig-derived BM-MSCs was performed by electroporation. Cells were evaluated for hPDX1 expression by immunofluorescence and RT-PCR. Transdifferentiation into pancreatic cells was confirmed by morphological transformation, immunofluorescence, and endogenous pPDX1 gene expression. At 3~4 weeks after transduction, cell morphology changed from spindle-like shape to round shape, similar to that observed in cuboidal epithelium expressing EGFP. Results of RT-PCR confirmed the expression of both exogenous hPDX1 and endogenous pPDX1. Therefore, GalT KO pig-derived BM-MSCs transdifferentiated into pancreatic cells by transfection of hPDX1. The present results are indicative of the therapeutic potential of PDX1-expressing GalT KO pig-derived BM-MSCs in ${\beta}-cell$ replacement. This potential needs to be explored further by using in vivo studies to confirm these findings.

아동의 복합운동이 착지 시 하지 손상요인에 미치는 영향 (Effects of Combined Exercise on Injury Risk Factors of Lower Extremity during Landing)

  • 하성희;류시현;김주년;길호종;류지선;윤석훈;박상균
    • 한국운동역학회지
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    • 제24권2호
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    • pp.173-180
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    • 2014
  • The purpose of this study was to investigate the effect of combined exercise on injury risk factors of lower extremity during landing. Ten sports talented athletes participated in this study. Sports talented athletes participated in a combined exercise (sports talented exercise, coordination) for 16 weeks. A three-dimensional motion analysis was performed using eight infrared cameras (sampling rate of 100 Hz), one force plate, and electromyography system (sampling rate of 1000 Hz) during landing. Kinetic, and kinematics analysis including average impulsive force, angle of lower extremity, vertical stiffness, onset of muscle activation were calculated by Matlab2009a software. Paired t-test was performed at alpha=.05. The average impulsive force in landing phase was not statistically significant (t=-.748, p=.474). The hip joint angle was more decreased in post test compared to pre test (E1: t=2.682, p=.025, E2: t=5.609, p=.000, E3: t=2.538, p=.032). The knee joint (E1: t=-.343, p=.739, E2: t=1.319, p=.220, E3: t=.589, p=.570) and ankle joint (E1: t=.081, p=.937, E2: t=.784, p=.453, E3: t=.392, p=.704) angle were tended to decrease after combined exercise. The vertical stiffness was tended to decrease after combined exercise (t=1.972, p=.080). Onset of quadriceps femoris (t=.698, p=.503) and medial gastocnemius (t=1.858, p=.096) were tended to be faster than biceps femoris (t=-.333, p=.747) after combined exercise. Although thses findings were not statistically significant except on a hip joint angle, risk factors of lower extremity such as joint angle, vertical stiffness and onset of quadriceps femoris, medial gastrocnemius were positively changed after the combined exercise but an additional training for improved onset of biceps femoris would be required in the future.

Saprolegnia parasitica Isolated from Rainbow Trout in Korea: Characterization, Anti-Saprolegnia Activity and Host Pathogen Interaction in Zebrafish Disease Model

  • Shin, Sangyeop;Kulatunga, D.C.M.;Dananjaya, S.H.S.;Nikapitiya, Chamilani;Lee, Jehee;De Zoysa, Mahanama
    • Mycobiology
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    • 제45권4호
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    • pp.297-311
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    • 2017
  • Saprolegniasis is one of the most devastating oomycete diseases in freshwater fish which is caused by species in the genus Saprolegnia including Saprolegnia parasitica. In this study, we isolated the strain of S. parasitica from diseased rainbow trout in Korea. Morphological and molecular based identification confirmed that isolated oomycete belongs to the member of S. parasitica, supported by its typical features including cotton-like mycelium, zoospores and phylogenetic analysis with internal transcribed spacer region. Pathogenicity of isolated S. parasitica was developed in embryo, juvenile, and adult zebrafish as a disease model. Host-pathogen interaction in adult zebrafish was investigated at transcriptional level. Upon infection with S. parasitica, pathogen/antigen recognition and signaling (TLR2, TLR4b, TLR5b, NOD1, and major histocompatibility complex class I), pro/anti-inflammatory cytokines (interleukin $[IL]-1{\beta}$, tumor necrosis factor ${\alpha}$, IL-6, IL-8, interferon ${\gamma}$, IL-12, and IL-10), matrix metalloproteinase (MMP9 and MMP13), cell surface molecules ($CD8^+$ and $CD4^+$) and antioxidant enzymes (superoxide dismutase, catalase) related genes were differentially modulated at 3- and 12-hr post infection. As an anti-Saprolegnia agent, plant based lawsone was applied to investigate on the susceptibility of S. parasitica showing the minimum inhibitory concentration and percentage inhibition of radial growth as $200{\mu}g/mL$ and 31.8%, respectively. Moreover, natural lawsone changed the membrane permeability of S. parasitica mycelium and caused irreversible damage and disintegration to the cellular membranes of S. parasitica. Transcriptional responses of the genes of S. parasitica mycelium exposed to lawsone were altered, indicating that lawsone could be a potential anti-S. parasitica agent for controlling S. parasitica infection.