• 제목/요약/키워드: ${\alpha}$-limit

검색결과 218건 처리시간 0.022초

환경준위 알파입자측정을 위한 다중선 비례계수기 개발(I) (Development of Multiwire Proportional Counter for Measurement of Environmental-level Alpha Particles)

  • 오필제;박태순;이민기;김경화
    • 분석과학
    • /
    • 제9권3호
    • /
    • pp.262-269
    • /
    • 1996
  • 저준위 및 환경준위의 알파입자 방출핵종의 방사능을 측정하기 위한 다중선 비례계수기를 개발하였다. 비례계수기의 외부 크기는 $350{\times}290{\times}30mm$이고, 측정이 가능한 측정선원의 최대 면적은 $250{\times}200mm$이다. 비례계수기의 재질은 최소의 background와 외부충격에 의한 형태변형을 방지하기 위해 스테인리스 스틸을 사용하였고, 양극선 및 음극선의 재질은 직경 $50{\mu}m$의 스테인리스 스틸 wire로서, 전체 전극선의 수는 각각 21개와 42개이다. 계수효율을 최대로 하고 검출기벽에 의한 흡수효과를 제거하기 위해서 측정선원은 검출기 내부에 위치하도록 하였으며, 선원두께에 의한 효율변화를 방지하기 위하여 선원 높이를 10mm까지 조절할 수 있도록 설계하였다. 제작된 검출기는 plateau, 작동전압, background, 계수효율, 선원위치에 따른 감도, 에너지분해능 등의 특성조사를 수행하였다. $^{241}Am$ 핵종의 경우, 시료 1L를 처리하여 50,000초를 측정하였을 때의 LLD(Lower Limit of Detection)는 5.0 mBq/L로 산출되어 ISO(International Organization for Standardization)에서 규정하고 있는 환경준위의 알파업자 측정용 장치의 LLD인 40mBq/L 보다 낮은 방사능을 분석할 수 있는 양호한 환경방사능 측정장치인 것으로 판명되었다.

  • PDF

UPLC를 이용한 염모제품에 함유된 유효성분에 대한 품질검사 (Quality inspections of active components in oxidative hair coloring products by UPLC)

  • 김동규;김유경;윤은선;홍미선;신지영;정윤경;김정헌;채영주;박승국
    • 분석과학
    • /
    • 제26권1호
    • /
    • pp.99-105
    • /
    • 2013
  • 염모제품 중 유효성분의 품질검사는 식품의약품안전청의 "의약외품에 관한 기준 및 시험방법"에 따라 제품에 표기된 산화염료들을 박층크로마토그래프법(TLC방법)으로 확인시험을 하도록 되어있다. 그러나 TLC방법은 원료분량이 미량이거나, $R_f$ 값이 비슷한 성분들이 존재하면 확인시험이 어려운 문제점이 있다. 본 연구에서는 미량성분 검출이 용이하며 분석시간이 짧다고 보고된 UPLC를 이용하기 위한 시료 전처리 및 분석조건을 탐색하였다. 유효성분들을 분석할 수 있는 검출한계는 6.7-77.9 ${\mu}g/L$, 정량한계는 22.3-259.7 ${\mu}g/L$ 이었으며, 회수율은 ${\alpha}$-naphthol를 제외하고는 96.2-101.5%로 양호하였다. 유효성분 추출하기 위한 시료 전처리에는 헥산-증류수를 사용하였다. 시료 전처리 시 복잡한 추출과정을 거치지 않고, UPLC방법은 빠르고 정확하게 염모제품에 함유되어 있는 유효성분들을 동시에 분석 할 수 있었다.

두릅 추출물의 기능성 원료 표준화를 위한 HPLC 분석법 검증 (HPLC analytical method validation of Aralia elata extract as a functional ingredients)

  • 안은미;최송암;최지영
    • 한국식품저장유통학회지
    • /
    • 제24권6호
    • /
    • pp.795-801
    • /
    • 2017
  • 개별인정형 건강기능식품 기능성 원료로 개발한 두릅추출물의 표준화를 위해 항고혈압활성 효과를 가지고 있는 기능성분 HE를 지표성분으로 설정하고, HPLC/UV 방법을 이용하여 기능성분 분석법을 확립하며 그에 따른 유효성 검증을 실시하고자 하였다. 유효성 검증 결과, 본 시험법에서 표준용액의 피크유지시간과 두릅추출물의 피크유지시간이 일치하였다. 검량선의 결정 계수(R2)는 0.9999 이상의 높은 직선성을 보여 분석에 적합함을 알 수 있었으며, 검출 한계는 $12.0{\mu}g/mL$, 정량한계는 $36.5{\mu}g/mL$로 두릅 추출물 중의 HE 함량분석을 위한 충분한 한계 수준으로 확인되었다. 정확성에서 $98.8{\pm}0.865-104.7{\pm}2.536%$로 높은 수준의 HE 회수율을 보였으며, 평균 분석오차는 0.013%로서 기준인 10% 이내를 만족하였다. 또한 반복성에서는 평균 $101.5{\pm}0.4%$로 RSD 0.4%를 나타내었고, 실험실내 정밀성에서 RSD 0.2%, 완건성에서 RSD 0.2%를 나타내어 모두 적합함을 확인하였다. 한편, 제주산 두릅추출물 중에는 기능성 지표성분인 HE가 4.88% 함유되어 있는 것으로 확인되었다. 이상의 실험 결과, 두릅추출물의 항고혈압활성 기능성분인 HE의 HPLC/UV 분석방법은 특이성, 직선성, 정확성, 정밀성, 반복성, 완건성 등의 모든 항목에서 validation 기준에 적합한 시험 방법으로 확인되었다.

우리나라 일부 우유의 무기질 함량 조성에 관한 연구 (Composition of Mineral Contents in Korean Cow무s Milk)

  • 이영희;정문호
    • 한국환경보건학회지
    • /
    • 제30권1호
    • /
    • pp.29-40
    • /
    • 2004
  • This study was performed to investigate and assess the composition of mineral and macronutrient contents in Korean cow′s milk.48 individual farm raw milk, 10 plant raw milk and 29 market milk were collected from June to August in 2003. The minerals such as calcium(Ca), potassium(K), magnesium(Mg), sodium(Na), zinc(Zn), iron(Fe) and phosphorus(P) were determined by using atomic absorption spectrometer(AAS). The macronutrients such as fat, protein and lactose were tested by using IR spectrometer. The obtained analytical results of minerals(mg/100 g) and rnaetronutrients (%) are as follows:1. In case of raw cow′s milk ; Ca 113.56, K 144.09, Mg 10.86, Na 42.53, Zn 0.42, Fe 0.030, p 113.32, fat 3.85, protein 3.08, lactose 4.80,2. In case of market cow′s milk ; Ca 103.04, K 142.46, Mg 10.27, Na 43.21, Zn 0.40, Fe 0.034. p 97.30, fat 3.78, protein 3.05, lactose 4.70,3. In case of fortified market cow′s milk ; Ca 165.40, K 145.79, Mg 10.57. Na 42.55, Zn 0.57, Fe 0.414, p 94.68, fat 3.74, protein 3.08, lactose 4.68,4. In case of processed market cow′s milk ; Ca 134.72, K 142.74, Mg 10.33, Na 45.07, Zn 0.50, Fe 0.650, p 92.48, fat 3.72, protein 3.07, lactose 4.74. According to the group of market milk(milk, fortified market row′s milk, processed market cow′s milk), the mean concentration of Ca and Fe were significantly higher in fortified and processed milk than milk(p<0.05). There were no significant differences in macronutrient(fat, protein, lactose) and mineral contents between pasteurized milk and UHT(ultra high temperature) treated milk($\alpha$=0.05). The labeled "Nutritional Facts" of market milk were satisfied with "Labeling Standards for Livestock Products of Korea". The measured mean concentrations of Ca, Fe, Zn were generally higher than lower limit of labeled value(above 80% of labeled value). The mean concentration of sodium was lower than upper limit of labeled value(below 120% of labeled value).

KS C IEC 60079-10-1 규격의 무시할 수 있는 정도와 누출특성에 관한 연구 (Study on the Negligible Extent(NE) and Release Characteristic of KS C IEC 60079-10-1(2015) Standard)

  • 조필래;이향직;백종배
    • 한국안전학회지
    • /
    • 제35권2호
    • /
    • pp.111-117
    • /
    • 2020
  • When KS C IEC 60069-10-1(2015) standard is applied to estimate a hazardous area, the chart showing the relationship between a hazardous area distance and release characteristic is used as a guide to determine the extent of hazardous zones for various forms of release. Three release characteristic lines based on the three types of release as an unimpeded jet release with high velocity, a diffusive jet release with low velocity, and a release of heavy gases or vapours that spread along horizontal surfaces are given. As these characteristic lines have the low limit threshold, it is difficult to estimate the hazardous area distance when the value of release characteristic is under the low limit threshold. And KS C IEC 60079-10-1(2015) standard shows the concept for a zone of Negligible extent(NE) which can be considered as non hazardous area, but it is also difficult to apply the concept of a Negligible extent. The purpose of this paper is to suggest the guideline for the release characteristic to decide a hazardous area distance and the Negligible extent(NE) being considered as non-hazardous area when deciding a hazardous area distances by the KS C IEC 60079-10-1 standard.

FTIR과 XRD를 이용한 α-Quartz 분석법 비교 (Comparison of Analytical Methods for α-Quartz by FTIR and XRD)

  • 김부욱;이종성;최병순
    • 한국환경보건학회지
    • /
    • 제35권2호
    • /
    • pp.130-142
    • /
    • 2009
  • This study compared FTIR with XRD method for the analysis of quartz by % recovery, coefficient of variation (CV) and influence of the interference. the results were as the following. 1. In FTIR method, the coefficient of determination ($r^2$) was 0.9998 in a calibration curve of $695\;cm^{-1}$, and the limit of detection was $4.9{\mu}g/sample$. 2. The highest recovery was $799\;cm^{-1}$ (98.2%). 3. The CVpooled of the FTIR method was approximately 10% in three wave numbers. 4. The analysis of qualitative and quantitative for quartz is difficult with mixed cristobalite and iron oxide. 5. In XRD method with rotating sample holder and LynxEye detector, the coefficient of determination was 0.9996 in a calibration curve, and the limit of detection was $5.9{\mu}g/sample$. 6. The recovery and CV pooled were 104.3%, and 11 %, respectively. 7. In muffle furnace ashing, the quartz weight decreased to 34% when the maximum weight of the iron oxide was more than eight times. In conclusion, the accuracy (% recovery) and precision (CV) of FTIR and XRD method for analyzing $\alpha$-quartz were similar. FTIR method was a disadvantage for sample matrix because it indicates possibility of interference. However, XRD method distinguished specific crystalline forms of silica, and the majority of silicate minerals. In addition, XRD method recommend filter dissolution to pretreatment method.

UHPLC를 이용한 새로운 한약제제 HPL-1의 품질관리법 개발 (Development of Quality Control Method for a Novel Herbal Medicine, HPL-1 using UHPLC)

  • 김세건;라마칸타라미차네;이경희;정현주
    • 대한본초학회지
    • /
    • 제30권3호
    • /
    • pp.19-24
    • /
    • 2015
  • Objectives : HPL-1, a novel herbal medicine which is composed of five herbs such as Kalopanacis Cortex, Chaenomelis Fructus, Raphani Semen, Atractylodis Rhizoma and Pulvis Aconiti Tuberis Purificatum, was developed for treatment of osteoarthritis. This study is aimed to develop analytical method for consistent quality control of HPL-1 and validate chromatographic method. Methods : Chromatographic analysis was performed using ultra-high performance liquid chromatography - diode array detector (UHPLC-DAD) equipped with RP-amide column, column oven, and auto sampler. Marker compounds [protocatechuic acid, chlorogenic acid, liriodendrin, 3,5-dicaffeoylquinic acid, ${\beta}$-D-(3-O-sinapoyl)-fructofuranosyl-$\alpha$-D-(6-O-sinapoyl)glucopyranoside and benzoylmesaconine] were separated by step gradient elution of acetonitrile and 0.1% phosphoric acid/water. The method validation was evaluated by quantitative validation parameters of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) according to KFDA guideline.Results : An optimized method for six marker compounds in HPL-1 was established by UHPLC-DAD. The correlation coefficient (R2) with each calibration curve was greater than 0.99. The LOD and LOQ were within the range of 0.008-0.090 and $0.023-0.274{\mu}g/mL$, respectively. The relative standard deviation (RSD) of intra- and inter-day variability were less than 4.0%. The result of recovery test was range from 93.3-106.3% with RSD < 4.0%.Conclusions : These results suggest that the quantitative UHPLC method is precise, accurate, effective for quality evaluation of HPL-1. The method may also contribute to improve quality of crude drug preparations used for treatment of various diseases.

Mg+Al2Ca 첨가 ADC12 (Al-Si-Cu) 합금의 미세조직, 인장 및 고주기 피로 특성 (Microstructure, Tensile Strength, and High Cycle Fatigue Properties of Mg+Al2Ca added ADC12 (Al-Si-Cu) Alloy)

  • 김영균;김민종;김세광;윤영옥;이기안
    • 소성∙가공
    • /
    • 제26권5호
    • /
    • pp.306-313
    • /
    • 2017
  • This study investigated the microstructure, tensile strength, and high cycle fatigue properties of ADC12 aluminum alloys with different $Mg+Al_2Ca$ contents manufactured using die casting process. Microstructural observation identified the presence of ${\alpha}-Al$, eutectic Si, $Al_2Cu$, and Fe-intermetallic phases. The increase of $Mg+Al_2Ca$ content resulted in finer pore size and decreased pore distribution. Room temperature tensile strength tests were conducted at strain rate of $1{\times}10^{-3}/sec$. For 0.6%Mg ADC12, measured UTS, YS, and El were 305.2MPa, 157.0MPa, and 2.7%, respectively. For 0.8%Mg ADC12, measured UTS, YS, and El were 311.2 MPa, 159.4 MPa, and 2.4%, respectively. Therefore, 0.8% ADC12 alloy had higher strength and slightly decreased elongation compared to 0.6% Mg ADC12. High cycle fatigue tests revealed that 0.6% Mg ADC12 alloy had a fatigue limit of 150 MPa while 0.8% Mg ADC12 had a fatigue limit of 160MPa. It was confirmed that $Mg+Al_2Ca$ added ADC12 alloy achieved finer, spherical eutectic Si particles, and $Al_2Cu$ phases with greater mechanical and fatigue properties since size and distribution of pores and shrinkage cavities decreased as $Mg+Al_2Ca$ content increased.

Direct Electrochemistry and Electrocatalysis of Myoglobin with CoMoO4 Nanorods Modified Carbon Ionic Liquid Electrode

  • Zhao, Zengying;Cao, Lili;Hu, Anhui;Zhang, Weili;Ju, Xiaomei;Zhang, Yuanyuan;Sun, Wei
    • Bulletin of the Korean Chemical Society
    • /
    • 제34권2호
    • /
    • pp.475-481
    • /
    • 2013
  • By using ionic liquid 1-hexylpyridinium hexafluorophosphate ($HPPF_6$) based carbon ionic liquid electrode (CILE) as the substrate electrode, a $CoMoO_4$ nanorods and myoglobin (Mb) composite was casted on the surface of CILE with chitosan (CTS) as the film forming material to obtain the modified electrode (CTS/$CoMoO_4$-Mb/CILE). Spectroscopic results indicated that Mb retained its native structures without any conformational changes after mixed with $CoMoO_4$ nanorods and CTS. Electrochemical behaviors of Mb on the electrode were carefully investigated by cyclic voltammetry with a pair of well-defined redox peaks from the heme Fe(III)/Fe(II) redox center of Mb appeared, which indicated that direct electron transfer between Mb and CILE was realized. Electrochemical parameters such as the electron transfer number (n), charge transfer coefficient (${\alpha}$) and electron transfer rate constant ($k_s$) were estimated by cyclic voltammetry with the results as 1.09, 0.53 and 1.16 $s^{-1}$, respectively. The Mb modified electrode showed good electrocatalytic ability toward the reduction of trichloroacetic acid in the concentration range from 0.1 to 32.0 mmol $L^{-1}$ with the detection limit as 0.036 mmol $L^{-1}$ ($3{\sigma}$), and the reduction of $H_2O_2$ in the concentration range from 0.12 to 397.0 ${\mu}mol\;L^{-1}$ with the detection limit as 0.0426 ${\mu}mol\;L^{-1}$ ($3{\sigma}$).

장딴지근 길이가 동적 균형 및 발바닥의 앞뒤 압력 분포에 미치는 영향 (Effects of Gastrocnemius Muscle Length on the Dynamic Balance and Antero-posterior Pressure Distribution of Foot)

  • 이원휘
    • 한국산학기술학회논문지
    • /
    • 제20권6호
    • /
    • pp.150-157
    • /
    • 2019
  • 본 연구의 목적은 장딴지근 길이가 동적 균형과 발의 앞뒤 압력분포에 어떤 영향을 미치는지 알아보는 것이다. 연구기간은 2018년 12월 3일부터 21일까지로 30명의 연구대상자를 장딴지근 길이 검사를 통해 장딴지근의 정상길이를 가진 대조군과 장딴지근 길이의 단축이 있는 실험군으로 분류하였다. 동적 균형과 발의 앞뒤 압력분포는 Biorescue장비를 사용하여 평가하였으며 동적 균형을 평가하기 위해 앞쪽과 뒤쪽 방향의 안정성 한계를 데이터로 수집하였다. 군간 동적 균형과 발의 앞뒤 압력분포에 유의한 차이가 있는지 알아보기 위하여 독립 t 검정을 이용하여 분석하였고, 통계학적 유의수준은 0.05로 하였다. 연구 결과 군간 동적 균형과 발의 앞뒤 압력분포는 유의한 차이가 있었다(p<0.05). 이러한 연구 결과를 통해 장딴지근 길이의 단축은 앞쪽으로 동적 균형 및 발의 앞쪽 압력 분포에 영향을 미친다는 것을 제안하고 그러므로 근골격계 질환 예방과 정상적인 균형능력을 위해 장딴지근의 적절한 길이를 유지하는 것은 중요하다.