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The Parallel Measurements of Stomatal Apertures and Apoplastic pH on Guard Cells from Epidermal Strips and Intact Leaves of Commelina communis

  • Lee, Joon-Sang
    • Korean Journal of Environmental Biology
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    • v.20 no.1
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    • pp.78-84
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    • 2002
  • The effects of light and darkness on stomatal aperture and guard cell apoplastic pH in the intact leaf and in the isolated epidermal strips of Commelina communis have been investigated. Stomata in the intact leaf opened wide in the light. In contrast, stomata in the isolated epidermal strips did not respond clearly to light. To eucidate the relationship between the stomatal aperture and the guard cell apoplastic pH, apoplastic pH was measured. In the light the guard cell wall of intact leaf was acidified by pH 1.9 units, falling from pH 7.3 to pH 5.4 in the first 10 minutes. On the contrary, apoplastic pH of isolated epidermal strips changed slowly from pH 7.3 to pH 6.9 at 20 min. Stomata in the intact leaf closed rapidly in the dark. On the other hand, stomata in the isolated epidermal strips failed to close in dark. There was a slow increase in apoplastic pH on transfer to the dark after incubation for 1.5 h in the light and the level observed before the experiment was regained after around 40 min. When the isolated epidermal strips were transferred to the dark, apoplastic pH maintained a uniform level of around pH 7.2-7.4. These results indicate that the mechanism of stomatal opening and closing from isolated epidermal strips and intact leaves could be different.

First reliable record of a stingray, Hemitrygon izuensis (Nishida and Nakaya, 1988) (Chondrichthyes: Dasyatidae) from Korea (한국산 색가오리과(Dasyatidae) 어류 1미기록종, Hemitrygon izuensis (Nishida and Nakaya, 1988))

  • JANG, Seo-Ha;KIM, Jin-Koo
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.57 no.3
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    • pp.205-213
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    • 2021
  • Two specimens of Hemitrygon izuensis (395.8-471.5 mm in disc width), belonging to the family Dasyatidae (Myliobatiformes), were collected from the waters off north-eastern Jeju-do Island in June 2016 and Pohang in August 2019. This species was characterized by having no spots on dorsal side of disc, white ventral tail fold and short preorbital snout length (15.6-18.3% of disc width). This species is similar to H. akajei and H. sinensis reported in Korea, but differs in small denticles on mid-line of dorsal surface of disc (absent in H. izuensis vs. present in H. akajei and H. sinensis), the length of ventral tail fold (28.1-31.0% in H. izuensis vs. 48.0-48.6% in H. akajei vs. 45.0% in H. sinensis), color of ventral tail fold (white in H. izuensis vs. black in H. akajei and H. sinensis) and small tubercles on the posterior part of tail (absent in H. izuensis vs. present in H. akajei and H. sinensis). We suggest a Korean name for H. izuensis as "I-ju-gal-saeg-ga-o-li" following Kim et al. (2019).

O$_3/H_2O_2$를 이용한 1,4-dioxane의 산화에 미치는 $H_2O_2$ 농도의 영향

  • Seo, Myeong-Gyo;Gang, Dae-Jong;Park, Jin-Do;Lee, Hak-Seong;Seo, Jeong-Ho
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2005.11a
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    • pp.143-146
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    • 2005
  • O_$3/H_2O_2$를 이용하여 1,4-dioxane의 산화처리에 미치는 $H_2O_2$ 농도의 영향에 대하여 고찰하였다. 또한 1,4-dioxane의 생물학적 분해가능성도 파악하였다. 실험은 bubble column reactor를 이용하여 수행되었으며, 여러 pH 및 $H_2O_2$ 조건에서 수행되었다. $H_2O_2$ 농도가 40 - 120 mg/L 범위에서, 초기 $H_2O_2$ 농도가 높을수록 1,4-dioxane의 제거율 및 생물분해성도 증가하였다.

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Correlation of Acoustic Cues in Stop Productions of Korean and English Adults and Children

  • Kong, Eun-Jong;Weismer, Gary
    • Phonetics and Speech Sciences
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    • v.2 no.4
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    • pp.29-37
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    • 2010
  • Previous studies have investigated a between-category relationship of multiple acoustic cues for a laryngeal contrast by examining the distributions of VOT, f0 and H1-H2. The current study examined within-category correlations between cues comprising stops by Korean- and English-speaking adults and children to understand how children master the internal structure of stop phonation types in two languages. Word-initial stops were collected from about 70 children and 15 adults speaking English and Korean, and were analyzed in terms of VOT, f0 and H1-H2 to compute correlation coefficients. Findings in adults' productions included a gender-differentiated cue-correlation pattern associated with H1-H2 in Korean tense stops and a trading relationship between f0 and VOT in Korean lax and aspirated stops and English voiced and voiceless stops. Children did not necessarily have adult-like cue-correlation patterns even in early-acquired categories, suggesting that the mastery of intra-category structure of phonation type might occur later than inter-category structure.

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Synthesis of Hexagonal Boron Nitride Nanosheet by Diffusion of Ammonia Borane Through Ni Films

  • Lee, Seok-Gyeong;Lee, Gang-Hyeok;Kim, Sang-U
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.252.1-252.1
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    • 2013
  • Hexagonal boron nitride (h-BN) is a two dimensional material which has high band-gap, flatness and inert properties. This properties are used various applications such as dielectric for electronic device, protective coating and ultra violet emitter so on. 1) In this report, we were growing h-BN sheet directly on sapphire 2"wafer. Ammonia borane (H3BNH3) and nickel were deposited on sapphire wafer by evaporate method. We used nickel film as a sub catalyst to make h-BN sheet growth. 2) During annealing process, ammonia borane moved to sapphire surface through the nickel grain boundary. 3) Synthesized h-BN sheet was confirmed by raman spectroscopy (FWHM: ~30cm-1) and layered structure was defined by cross TEM (~10 layer). Also we controlled number of layer by using of different nickel and ammonia borane thickness. This nickel film supported h-BN growth method may propose fully and directly growing on sapphire. And using deposited ammonia borane and nickel films is scalable and controllable the thickness for h-BN layer number controlling.

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Preparation of Porous Silica-Pillared Montmorillonite: Simultaneous Intercalation of Amine-Tetraethylorthosilicate into H-Montmorillonite and Intra-Gallery Amine-Catalyzed Hydrolysis of Tetraethylorthosilicate

  • Gwon, O Yun;Park, Gyeong Won;Jeong, Sun Yeong
    • Bulletin of the Korean Chemical Society
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    • v.22 no.7
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    • pp.678-684
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    • 2001
  • Porous silica-pillared montmorillonites were prepared by simultaneous intercalation of dodecylamine-TEOS [tetraethylorthosilicate, Si(OC2H5)4] into the H-montmorillonite and intragallery amine-catalyzed hydrolysis of TEOS. Mixtures of the H-montmorillonite, dodecylamine and TEOS at molar ratios of 1 : 2 : 15-30 and 1 : 2-6 : 20 resulted to swollen and viscous gel once at room temperature, allowing intercalation compounds which dodecylamine and TEOS were simultaneously intercalated into interlayer of H-montmorillonite. The hydrolysis of the gallery TEOS was conducted in water solution for 40 min at room temperature, affording siloxane-pillared H-montmorillonite. Calcination of samples at 500 $^{\circ}C$ in air resulted in silica-pillared montmorillonite with large specific surface areas between 403 and 577 m2 /g, depending on the reaction stoichiometry. The reaction at H-montmorillonite : dodecylamine : TEOS reaction stoichiometries of 1 : 2 : 15 and 1 : 4 : 20 resulted in high specific surface areas and mesopores with a narrow pore size distribution. Result indicates that the intragallery-amine catalyze the hydrolysis of gallery-TEOS and simultaneously have a role of gallery-templated micellar assemblies.

H0 Determination Using TRGB Distances to the Virgo Infalling Galaxies

  • Kim, Yoo Jung;Kang, Jisu;Lee, Myung Gyoon;Jang, In Sung
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.32.1-32.1
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    • 2020
  • An independent determination of H0 is crucial given the growing tension of the Hubble constant (H0). In this work, we present a new determination of H0 using velocities and Tip of the Red Giant Branch (TRGB) distances to 33 galaxies in front of the Virgo Cluster. We model the infall pattern of the local Hubble flow modified by the Virgo mass, as a function of the H0, the radius of the zero-velocity surface R0, and the intrinsic velocity scatter. Fitting velocities and TRGB distances of 33 galaxies to the model, we obtain H0 = 65.6 +/- 3.4 (stat) +/- 1.0 (sys) km/s/Mpc and R0 = 6.96 +/- 0.35 Mpc. Our local H0 is consistent with the global H0 determined from cosmic microwave background radiation, showing no tension.

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Acute and subacute toxicity of trichlorfon in guppies (Poecilia reticulata)

  • Heo, Gang-Joon;Shin, Gee-Wook
    • Korean Journal of Veterinary Research
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    • v.49 no.3
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    • pp.253-256
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    • 2009
  • The aim of the present study was to determine the acute and subacute potential of trichlorfon in guppies (Poecilia reticulatus). We first defined the 24 h median tolerance limit ($TLm_{24h}$) of the fish to trichlorfon. Guppies were then treated with $TLm_{24h}$ and 1/10 $TLm_{24h}$ trichlorfon concentrations to evaluate if any histological alterations occurred. The $TLm_{24h}$ value of trichlorfon was 11 ppm. This concentration resulted in acute toxicity to the gills and kidneys with edema, hyperplasia of the gill lamellae, and vacuolated degeneration and necrosis of renal tubular cells. In the case of subacute toxicity using a 10-fold dilution of the $TLm_{24h}$ value (1.1 ppm), no behavioral changes, external lesions or histopathological changes were observed. Therefore, safe concentration of trichlorfon might be 1.1 ppm in guppy for controlling parasitic infections.

Biochemical Properties of NAD(P)H-Quinone Oxidoreductase from Saccharomyces cerevisiae

  • Kim, Kyung-Soon;Suk, Hee-Won
    • BMB Reports
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    • v.32 no.2
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    • pp.127-132
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    • 1999
  • The NAD(P)H-quinone oxidoreductase (EC 1. 6. 99. 2) was purified from S. cerevisiae. The native molecular weight of the enzyme is approximately 111 kDa and is composed of five identical subunits with molecular weights of 22 kDa each. The optimum pH of the enzyme is pH 6.0 with 1,4-benzoquinone as a substrate. The apparent $k_m$ for 1,4-benzoquinone and 1,4- naphthoquinone are 1.3 mM and $14.3\;{\mu}M$, respectively. Its activity is greatly inhibited by $Cu^{2+}$ and $Hg^{2+}$ ions, nitrofurantoin, dicumarol, and Cibacron blue 3GA. The purified NAD(P)H-quinone oxidoreductase was found capable of reducing aromatic nitroso compounds as well as a variety of quinones, and can utilize either NADH or NADPH as a source of reducing equivalents. The nitroso reductase activity of the purified NAD(P)H-quinone oxidoreductase is strongly inhibited by dicumarol.

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Effect of pH and Reaction Temperature on Mechanical Properties of Hydroxyapatite for Dental prosthesis (치관보철용 Hydroxyapatite의 기계적 물성에 미치는 pH 및 반응온도의 영향)

  • Chung, In-Sung;Her, Ho-Young
    • Journal of Technologic Dentistry
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    • v.26 no.1
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    • pp.89-96
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    • 2004
  • Hydroxyapatite powder were synthesized by precipitation method, varying pH, and reaction temperature. The powders were heated at 1,200$^{\circ}{\cdots}$ and 1,300$^{\circ}{\cdots}$ for fabrication of dental prosthesis. The results are as follows: Synthesized powder showed the smallest particle in size, under the conditions of pH 11 and reaction temperature 37$^{\circ}{\cdots}$. The hydroxyapatite was partially converted to $\alpha,\;\beta$-TCP at 1,200$^{\circ}{\cdots}$ and 1,300$^{\circ}{\cdots}$. Mechanical strength of sample was affected by such powder preparation conditions as pH and reaction temperature and sintering temperature. The mechanical strength of sample prepared under the same conditions was increased with increasing pH, reaction temperature and sintering temperature.

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