• Title/Summary/Keyword: $^{14}C$ and $^{15}N$

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EFFECT OF SOFT CHELATING IRRIGATION ON THE SEALING ABILITY OF GP/AH PLUS ROOT FILLINGS (Soft chelating irrigation이 GP/AH Plus로 충전된 근관의 sealing ability에 미치는 영향에 대한 평가)

  • Yu, Yi-Suk;Kim, Tae-Gun;Lee, Kwang-Won;Yu, Mi-Kyung
    • Restorative Dentistry and Endodontics
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    • v.34 no.6
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    • pp.484-490
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    • 2009
  • The purpose of this study was to evaluate the effect of soft chelating irrigant on the sealing ability of root fillings by using a glucose leakage test. A total of 45 single-rooted teeth were selected for the study. The teeth were decoronated leaving a total length of 13mm. The root canals prepared using K3 NiTi rotary instruments to an apical dimension of size 45(0.06 taper). The specimens were then randomly divided into 3 experimental groups of 13 roots each and 2 control groups of 3 roots each. Specimen in each group were prepared with different irrigation protocols : group 1, 2.5% NaOCl; group 2, 2.5% NaOCl and 17% EDTA: group 3, 2.5% NaOCl and 15% HEBP. The root canals were filled with gutta-percha and AH Plus sealer using lateral condensation. After 7 days in $37^{\circ}C$, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometry at 1, 4, 7, 14, 21 and 28 days. There was a tendency of increase in leakage in all experimental groups during experimental period. HEBP-treated dentin showed no significant difference with EDTA-treated dentin during experimental period. From the 21th day onward, HEBP-treated dentin showed significantly lower leakage than smear-covered dentin. HEBP-treated dentin displayed a similar sealing pattern to EDTA-treated dentin and a better sealing ability than smear-covered dentin. Consequently, a soft chelator(HEBP) could be considered as the possible alternative to EDTA.

An Assessment of Allograft Function in Canine Single Lung Transplantation (일측 폐이식 실험견에서 이식폐의 기능 평가연구)

  • 박창권
    • Journal of Chest Surgery
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    • v.28 no.12
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    • pp.1096-1106
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    • 1995
  • We experienced 7 cases of left single lung transplantation in 14 mongrel dogs and analyzed graft lung function by hemodynamics, blood gas analysis, chest X-ray, biopsy and perfusion lung scan. We performed right pulmonary artery cuff[PA cuff for analysis of graft lung function in 3 cases. The donor lungs were flushed with modified Euro-Collins solution[n=3 or low potassium dextran glucose solution[n=4 and preserved for 4 to 5 hours[n=4 or 24 hours[n=3 at 10o C and implanted to the dogs with similar weight . Assessment of left graft lung was done by occluding the right pulmonary artery for 10 minutes using PA cuff. Assessment for graft lung function was done immediately after an operation and after 3 days, 7days and 3 weeks postoperatively. Four dogs survived for 3days, 7days[2 cases and 3 weeks respectively. Other three dogs expired within 3 hours of reperfusion. Immediate perfusion scans of left lung in four survived dogs after reperfusion were 42.1%, 36% , 11% and 5.9% respectively, and another dog with 4.8% perfusion to left lung was dead due to left atrial thrombi after 3 hours reperfusion. In one case among three acute rejections follow-up perfusion scan was done on 3rd and 11th postoperative day and the result decreased from 36% perfusion immediate postoperatively to 21% and 15% respectively. Three expired dogs postoperatively couldn`t tolerate occlusion of right pulmonary artery with above 40 mmHg of mean pulmonary artery pressure. On the other hand, three survival dogs postoperatively tolerated occlusion of right pulmonay artery with less than 30 mmHg of mean pulmonary artery pressure. and one dog couldn`t tolerate same procedure immediate postopertively but in 2 hours reperfusion later tolerated with 29 mmHg of mean pulmonary artery pressure.In conclusion we couldn`t compare the effect of two flushing solutions but low potassium dextran glucose solution showed relatively safe preservation effect in cases with preservation of more than 20 hours. Also canine left single lung transplantation model with PA cuff indicated useful method for the assessment of graft lung function with effect of lung preservation.

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RUBBER INCLUSION EFFECTS ON MECHANICAL PROPERTIES OF RUBBER-ADDED COMPOSITE GEOMATERIAL

  • Kim, Yun-Tae;Gang, Hyo-Seb
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.09c
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    • pp.129-134
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    • 2010
  • This paper investigates effects of rubber inclusion on the strength and physical characteristics of rubber.added composite geomaterial (CGM) in which dredged soils, crumb rubber, and bottom ash are reused for recycling. Several series of test specimens were prepared at 5 different percentages of rubber content (i.e. 0%, 25%, 50%, 75%, and 100% by weight of the dry dredged soil) and three different percentages of bottom ash content (i.e. 0%, 50% and 100% by weight of the dry dredged soil). The mixed soil specimens were subjected to unconfined compression test and elastic wave test to investigate their unconfined compressive strengths and small strain properties. The values of bulk unit weight of the CGM with bottom ash content of 0% and 100% decrease from 14kN/$m^3$ to 11kN/$m^3$ and 15kN/$m^3$ to 12kN/$m^3$, respectively, as rubber content increases, because the rubber had a specific gravity of 1.13. The test results indicated that the rubber content and bottom ash content were found to influence the strength and stress-strain behavior of CGM. Overall, the unconfined compressive strength, and shear modulus were found to decrease with increasing rubber content. Among the samples tested in this study, those with a lower rubber content exhibited sand-like behavior and a higher shear modulus. Samples with a higher rubber content exhibited rubber-like behavior and a lower shear modulus. The CGM with 100% bottom ash could be used as alternative backfill material better than CGM with 0% bottom ash. The results of elastic wave tests indicate that the higher rubber content, the lower shear modulus (G).

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Monitoring of compositions of gamma-linolenic and omega-3 fatty acids in some functional foods consumed in market (유통중인 건강기능식품의 감마-리놀렌산 및 오메가-3 지방산 함량 모니터링)

  • Kim, Dae-Kyung;Shin, Jung-Ah;Lee, Ki-Teak
    • Korean Journal of Agricultural Science
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    • v.38 no.2
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    • pp.277-284
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    • 2011
  • The compositions of health functional food products (HFFP; 18 products) containing gamma linolenic acid (GLA; $C_{18:3}$, n-6) and omega-3 fatty acids (EPA and DHA) were investigated. The contents of index components (especially, GLA and omega-3) in HFFPs were monitored by GC-FID analysis. Among the GLA products (sample No. 1~8), the content of GLA in most samples (except sample No.6) ranged from 8.04 to 9.98 g/100 g. These results were suitable for the reference standard (more 7.0 g/100 g) of HFF. In the omega-3 products (sample No. 10 and 15) derived from harp seal oil (HSO), the total contents of EPA and DHA were 14.21-15.98 g/100 g, respectively. These values were suitable for the reference standard (more 12.0 g/100 g) of HFF. Besides, among the omega-3 products (sample No.9, 11~14, 16~18) derived from fish oil, the total content of EPA and DHA ranged from 24.11 to 31.20 g/100 g. These results were suitable for the reference standard (more 18.0 g/100 g) of HFF. In the result of TLC analysis, the HFFPs of 18 were mainly composed of triacylglycerols (TAGs). The content of trans fatty acid in 18 HFFPs was detected in less than 0.30 g/100 g. For the detection of trans fatty acid, $^1H$-NMR (600 MHz) can be used because chemical shift of trans fatty acid was observed at 5.3 ppm in this study.

Construction of Starch-assimilating and Ethanol-fermenting Yeast by Protoplast Fusion (원형질 융합에 의한 전분으로부터 에탄올 발효효모균주의 개량)

  • 이혜정;이지나;천경숙;박소영;마은애;민경희
    • Korean Journal of Microbiology
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    • v.30 no.6
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    • pp.546-552
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    • 1992
  • Ethanol-tolerant strain, S. eerevisiae BUI a26 ($alc^r thr^-$) and gJucoamylase-producing strain, S diastatieus AI5a6 (STA+ hom-) were prepared by means of genetic manipulation, Protoplast fusion was carried out to introduce STA gene from AI5a6 strain to BUla26 strain, Protoplast formation was shown at 0,8 M sorbitol and 200 Jig/ml to 400 Jig/ml zymolyase treatment for 2 hours incubation, Fusion frequency was $ 3.25 {\times} 10^{-3}$ to the regenerated protoplast number using PEG 6000 for 90 min incubation. The excellent fusants with genotype of STA- $alc^r thr^-$ hom+/STA+ ($alc^s thr^+$ hom- (2n), F7 and FIO, were selected by ethanol-tolerant, ethanol fermentation, and glucoamylase production tests, Glucoamylase production of AI5a6 showed 2,7 units, but 4.2 or 8.4 units for F7 or FIO fusant at $30^{\circ}C$, Ethanol fermentation from 32% glucose by BUla26 was 14,0%(v/v) in fermentaion medium for 5 days incubation, but 14.5% or 15,0% for F7 or FIO strain, respectively. Ethanol fermentation from 5% starch was 2,0% by F7, or 1.8% by FIO strain in fermentation medium for 5 days fermentation.

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Effect of Backfat Thickness on the Carcass Grade Factors and Carcass Price in Hanwoo Cows and Steers (등지방 두께가 한우 암소와 거세우의 도체 등급 요인 및 도체가격에 미치는 영향)

  • Lee, Jong-Moon;Choe, Ju-Hui;Park, Hyun-Kyung;Kim, Yuen-Ho;Park, Beom-Young;Kim, Kwan-Tae;Koh, Kyung-Chul;Seo, Sang-Chul;Hwang, Kyu-Seok
    • Food Science of Animal Resources
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    • v.31 no.2
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    • pp.280-289
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    • 2011
  • Analyses were conducted to estimate the effect of backfat thickness (BFT) on carcass grade factors and carcass price in Hanwoo cows and steers. Data on grade factors and prices were collected from 69,159 Hanwoo carcasses (n = 13,376 cows, n = 55,783 steers) abtained at the Nonghyup Seoul slaughter house in 2009. Carcass BFTs were divided into nine categories from ${\leq}$ 3 mm to ${\qeq}$ 25 mm. The average BFTs were 11.93 mm in cows and 12.57 mm in steers. An increase in the BFT resulted in an increase in carcass weight, loin-eye area, skeletal maturity, fat color, but decreases in the yield index and yield grade (from grade A to C) in cows and steers. Carcass unit price (Won/kg) improved if the carcass was sold with a BFT of 13-15 mm in cows and 10-12 mm in steers. Marbling score and quality grade increased significantly to 21 mm BFT but decreased from a BFT of 22 mm in cows and steers. BFT was significantly correlated with yield index (cow, r = $-0.97^{**}$; steer, r = $-0.96^{**}$), marbling score (cow, r = $0.20^{**}$; steer, r = $0.14^{**}$), and carcass price (cow, r = $0.03^{**}$; steer, r = $-0.11^{**}$). These results could be used as a fundamental dataset for further research to increase the productivity of Hanwoo carcasses.

Callus Induction and Differentiation from Rice (Oryza Sativa L.) Anthers (벼 약(葯)으로부터 callus 형성(形成)과 분화(分化)에 대(對)하여)

  • Kim, Dal Ung;Bae, Min Gyu
    • Current Research on Agriculture and Life Sciences
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    • v.1
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    • pp.1-9
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    • 1983
  • This study was conducted to obtain basic information on the rice anther culture. Materials used were (Inabawase X YR 2404-14-2-1) $F_1$ hybrid. Callus growth rate on various media, induction frequency of callus in spikelet and panicle, and the effect of treatment on anther and callus were evaluated. The results obtained were summarized as follows ; The growth rate of callus on N-6, M-S, P.E.agar media was 19.8, 13.1, 4.1 times respectively after 30 days inoculated, and on liquid media was 3.8, 5.1, 1.4 times, respectively. Organ differentiation on N-6, M-S, P.E.agar media was 37.5%, 12.5%, 17.5% respectively. The difference of induction frequency of callus per panicle was 0.14%-6.25% and per spikelet was 0-19.05%. Almost callus was induced 30-35 days after inoculation. Organ differentiation of induced callus was decreased by culture. Callus cultured for 13 days after induction did not make shoot. Anthers cold shocked at $8^{\circ}C$ for 5 days obtained 3.32% efficiencys of callus induction per number of anthers plated, and compared with 2.41% of no treated anthers. But anther treated at $8^{\circ}C$ for 7 days decreased 2.24%. Callus induction periods were shortened by cold treatment for about 5 days. Callus cultured on medium containing 2 mg/l of 2, 4-D showed 5% on root formation but medium containing 5 mg/l of 2, 4-D showed 30% of root formation after transfered on the medium without 2, 4-D. Callus cold shocked at $15-18^{\circ}C$ revealed poor efficiency for root formation, but 5 days treatment was good for shoot formation.

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Foraminoplastic Superior Vertebral Notch Approach with Reamers in Percutaneous Endoscopic Lumbar Discectomy : Technical Note and Clinical Outcome in Limited Indications of Percutaneous Endoscopic Lumbar Discectomy

  • Lee, Chul-Woo;Yoon, Kang-Jun;Ha, Sang-Soo;Kang, Joon-Ki
    • Journal of Korean Neurosurgical Society
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    • v.59 no.2
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    • pp.172-181
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    • 2016
  • To describe the details of the foraminoplastic superior vertebral notch approach (FSVNA) with reamers in percutaneous endoscopic lumbar discectomy (PELD) and to demonstrate the clinical outcomes in limited indications of PELD. Retrospective data were collected from 64 patients who underwent PELD with FSVNA from August 2012 to April 2014. Inclusion criteria were high grade migrated disc, high canal compromised disc, and disc protrusion combined with foraminal stenosis. The clinical outcomes were assessed using by the visual analogue scale (VAS), Oswestry Disability Index (ODI) and modified MacNab criteria. Complications related to the surgery were reviewed. The procedure used a unique approach, using the superior vertebral notch as the target and performing foraminoplasty with only reamers under C-arm control. The mean age of the 55 female and 32 male patients was 52.73 years. The mean F/U period was $12.2{\pm}4.2$ months. Preoperative VAS ($8.24{\pm}1.25$) and ODI ($67.8{\pm}15.4$) score improved significantly at the last follow-up (VAS, $1.93{\pm}1.78$; ODI, $17.14{\pm}15.7$). Based on the modified MacNab criteria, excellent or good results were obtained in 95.3% of the patients. Postoperative transient dysthesia (n=2) and reoperation (n=1) due to recurred disc were reported. PELD with FSVNA could be a good method for treating lumbar disc herniation. This procedure may offer safe and efficacious results, especially in the relatively limited indications for PELD.

A Study on Aerobic Composting of Food Waste with Controlling Temperature by Air Flow Rate (온도를 공기량으로 제어한 음식물 쓰레기 호기성 퇴비화에 관한 연구)

  • Hwang, Seon-Hyeon;Sin, Chang-Ho;Sin, Bu-Yeong;Jo, Mu-Hwan
    • KSBB Journal
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    • v.14 no.5
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    • pp.621-627
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    • 1999
  • A food waste composting apparatus of 450 L was designed for and tested with changing conditions of inlet air flow rate, agitation and inoculation. Agitation was done twice per day for 5 min and inlet air flow rate was set as 22.7 L/min for RUN 1. For RUN 2 and RUN 3, agitation was continuous, and inlet air flow rate was changed frequently as 10 L/min, 15 L/min and 20 L/min in order to maintain temp. above 5$0^{\circ}C$, and the concentration of $O_2$over 7 mol%. The compost of RUN3 was inoculated with 10 wt% of accomplished compost, and it was compared with RUN 1 and 2 to show the effect of inoculation. The composting rates of RUN 2 and RUN 3 were faster than that of RUN 1, because agitation was continuous and temperature was controlled in RUN 2 and RUN 3. Inoculated RUN 3 was better than RUN 1 and RUN 2 in the concentrations of $CO_2$and reduction of volatile solids. while the effect of inoculation on C/N ratios, pH change and the numbers of microoragnisms was not clearly appeared.

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Effects of various bed soil on the growth and yield of organically grown 2-year-old ginseng in the shaded plastic houses (하우스 인삼재배에서 상토의 조성이 유기농 2년근 인삼의 생육 및 수량에 미치는 영향)

  • Lee, Nu-Ri;Kim, Jung-Sun;Jo, Seo-Ri;Choi, Yeong-Kyu;Choi, Jae-Eul
    • Korean Journal of Agricultural Science
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    • v.39 no.1
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    • pp.35-41
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    • 2012
  • This research was conducted to investigate the influence of organic bed soil substrates on growth and yield of organically grown ginseng transplantation in a shaded plastic houses. The pH and EC of the substrates used for this study were 5.93-6.78 and 0.03-0.15 dS/m, respectively. The concentrations of NH4-N and $NO_3$-N were 14.01-68.63 mg/L, 5.60-58.83 mg/L respectively. and the average quantum in the shaded plastic houses was 11-15% of natural light. The maximum temperature in the shaded plastic houses is higher ($3-7^{\circ}C$) than that of outside open field from the last part of April to early in August. Emergence date of ginseng was on March 21 in the mongolian type shaded plastic house, and was on March 29 in normal type shaded plastic house. Both roots and shoot growth of ginseng were excellent in the bed soils with PPV-2, compared with other compounds used. We concluded that the PPV-2 could be promising a good bed soil substrate for organic ginseng cultivation in shaded plastic house.