• Natural Product Sciences
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• 제4권2호
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• pp.78-83
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• 1998

Chemical investigation on a sample of the needles of Himalayan Taxus baccata has resulted in the isolation of several taxoids including taxol (1) 10-deacetyl-baccatin III (2) and 2-deacetoxytaxinine J (3) along with different lignans (6 and 7) and simple phenolics (8, 9, 10, 11 and 12). The occurrence of 4-(4'-hydroxyphenyl)-butane-2-one and 4-(4'-hydroxyphenyl)-trans-but 3-ene-2-one (8) in Taxus species is reported for the first time. The $^{13}C-NMR$ spectral data of two rearranged taxiod constituents, brevifoliol (4) and 13-decinnamoyltaxchinin B (5) are presented. The acid-catalyzed decomposition of taxol has been discussed. The synthesis of other two constituents, rhododendrol (10) and hibalactone (7) has been described.

• 한국환경과학회지
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• 제26권11호
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• pp.1285-1295
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• 2017

Chrysanthemum boreale Makino is widely distributed in Korea, China, Japan and Southeast Asian countries. C. boreale is one of the herbs used for treating various inflammatory diseases in oriental medicine. The present study was conducted to identify biologically active compounds from the leaves and stems of C. boreale. We isolated two sesquiterpene sactones from the leaves and stems of C. boreale using silica gel column chromatography and recyclic high perfomance liquid chromatography. The lactones were characterized by their spectroscopic data (NMR, IR, MASS). These compounds were subjected to Farnesyl Protein Transferase (FPTase) inhibition, Nitric Oxide (NO) release inhibition and apoptosis inhibition. The structur of the following isolated compound were elucidated 8,10-${\small{O}$-Acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 4,10-dihydroxy-8-${\small{O}$-Acetyl-2,11(13)-guaiadiene-12,6-olide. In the NO release inhibition assay, compound 2 showed strong activities, with an $IC_{50}$ value of $7{\mu}g/mL$, whereas compound 1 did not exhibit significant activity with an $IC_{50}$ value of over $14{\mu}g/mL$ against murine macrophage.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1988년도 학술대회지
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• pp.33-35
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• 1988

우리는 경험적으로 인삼이 훌륭한 치료효과로서 항종양효과가 있다는 것을 알고 있다. 인삼으로부터 종양발육 억제효과가 있는 물질을 분리하는 과정에서 우리는 새로운 타입의 항종양 물질을 발견하였다. 이물질은 Panax ginseng C.A. Mayer의 분말로부터 분리하였는데 이 분말은 일본에서 홍삼분말이라는 이름으로 의약품으로서 여러가지 질병치료에 사용되고 있다. IR, $^{1}H,\;^{13}C-NMR$ 및 MS에 의한 분석결과로 이 물질은 panaxytriol로 동정 되었다. 고려홍삼에서 분리된 panaxytriol은 in vitro 시험에서 몇가지의 인체암세포와 악성백혈병 세포의 성장을 억제하였다. 비록 panaxytriol에 의한 세포성장억제에 대한 상세한 작용기전은 알려져있지 않으나 panaxytriol의 효과는 처리시간보다는 농도 의존성이 있는 것으로 밝혀졌다.

• 한국미생물·생명공학회지
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• 제45권4호
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• pp.311-315
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• 2017

화살나무 가지로부터 activity based fractionation에 의해 ${\alpha}$-glucosidase 저해 활성 물질 compound 1-4을 분리하였고, $^1H$ NMR, $^{13}C$ NMR, $^1H-^1H$ COSY와 HMBC 등의 spectral data에 의해 구조를 결정하였다. Compound 1-4는 모두 flavonol 물질로 ${\alpha}$-glucosidase에 대해 $IC_{50}$ 값이 각각 25.3, 17.1, 47.3과 $35.1{\mu}M$로 positive control로 사용한 acarbose 보다 강한 활성을 나타내었다. 화살나무의 혈당 저하 기능의 유효성분으로 처음 동정된 Compound 1-4는 ${\alpha}$-glucosidase에만 특이적 활성을 갖는 물질로 당뇨병 치료제로의 개발 가능성이 높은 물질로 사료된다.

• 농약과학회지
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• 제6권1호
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• pp.31-35
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• 2002

본 논문의 목적은 benfuracarb 원제(90.2%)에 함유된 불순물의 glutathione-S-transferase와 amidase에 대한 저해 특성과 해당 불순물의 구조를 밝히는데 있다. Benfuracarb 원제, 유효성분 및 불순물은 glutathione-S-transferase(GST)를 효과적으로 저해하였으나, 그 저해력은 GST 효소 저해제인 ethacrynic acid의 저해력보다는 낮았다. 즉, GST에 대한 benfuracarb 원제, 유효성분 및 불순물의 $I_{50}$은 각각 $9.7{\times}10^{-4}M,\;>1.0{\times}10^{-3}M,\;1.8{\times}10^{-4}M$이었으나, ethacrynic acid의 $I_{50}$은 $1.7{\times}10^{-5}M$이었다. Benfuracarb 원제, 유효성분 및 불순물은 amidase를 저해하였는데, 이들의 효소저해력은 iprobenfos의 저해력($I_{50},\;8.2{\times}10^{-7}M$)보다는 낮은 $6.0{\times}10^{-5}M,\;4.3{\times}10^{-4}M,\;7.6{\times}10^{-5}M$이었다. Benfuracarb 원제에는 4종의 불순물(IM $1{\sim}4$)이 검출되었는데, 이들 중 IM 2와 3은 GST와 amidase의 활성을 저해하였던 반면, IM 4는 효소활성을 저해하지 않았다. 이들 불순물 중 효소활성 저해특성을 갖는 IM 2와 3을 IR, $^1H$-NMR, $^{13}C$-NMR, LC-MS를 이용하여 구조를 분석한 결과, IM 2는 ethyl-N-isopropylamino propionate로, 그리고 IM 3은 ethyl-N-isopropyl-N-(chlorosulfenyl) aminopropionate로 확인되었다.

• Natural Product Sciences
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• 제11권4호
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• pp.196-198
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• 2005

Four ursane type triterpenes have been isolated from the methanolic extract of the aerial parts of Agrimonia pilosa Ledeb. (Rosaceae) through repeated silica gel and reverse-phase C-18 column chromatography. Their chemical structures were elucidated as ursolic acid (1), pomolic acid (2), tormentic acid (3), and corosolic acid (4) on the basis of their MS, $^1H-$, and $^{13}C-NMR$ spectral data. Compounds 2 and 4 were isolated from the genes of Agrimonia for the first time.

• 생약학회지
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• 제39권4호
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• pp.305-309
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• 2008

Dehydrocorydaline was isolated from the roots of Corydalis ternata (Papaveraceae) and identified by the comparison the $^1H-$ and $^{13}C$-NMR spectral data with those of authentic sample. The content of dehydrocorydaline was determined by the HPLC analysis based on extraction of ground plant material. Quantitative analysis of dehydrocorydaline in MeOH extract of C. ternata by HPLC showed $1.31{\pm}0.95%$ in 20 samples collected throughout regions of Korea.

• 대한화학회지
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• 제56권4호
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• pp.459-463
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• 2012

8-Formyl-4-Methyl-7-Hydroxy Coumarin Derivatives were synthesized via Penchem condensation followed by Duffs reaction. Treatment of this with N,N-di substituted cyano acetamides in the presence of piperdine afforded New 8-Formyl-4-Methyl-7-Hydroxy Coumarin Derivatives (7a-o). Their structures were characterized by IR, $^1H$ and $^{13}C$ NMR and Mass spectral and elemental analysis data.

• 생약학회지
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• 제24권1호
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• pp.32-37
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• 1993

Phytochemical studies of the root of Aristolochia contorta afforded an unidentified N-glycoside rarely found in natural products. It's structure was elucidated as 8-desmethoxy-aristolactam $N-{\beta}-D-glucopyranoside$ by spectral data and chemical analysis. 6-Hydroxy-8-desmethoxy-aristolactam $N-{\beta}-D-glucopyranoside$, aristolactam I and aristolactam AII were also isolated from the same source. $^{13}C-NMR$ spectra were first assigned and the result confirmed N-C-O-glycosidic linkages in the glycosides.

• 대한화학회지
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• 제57권3호
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• pp.341-351
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• 2013

Zn(II), Cd(II) and Hg(II) complexes with a general composition[$M(L)_2(X)_2$], where L=4-aminopyridine (4AP) and $X=NO_2{^-}$ were prepared under microwave irradiation. The metal complexes were characterized by elemental analyses, molar conductance, IR, Far-IR, electronic, NMR ($^1H$, $^{13}C$), XPS spectral and thermal studies. The spectroscopic studies reveal the composition, different modes of bonding, electronic transition, different chemical environment of C and H atoms and the electronic state of the metal atoms. On the basis of the characterization data, tetrahedral geometry is suggested for all the complexes. The free ligand (4-aminopyridine) and their metal complexes were screened against phytopathogenic fungi and bacteria in vitro and the activities were compared.