• Title/Summary/Keyword: $\gamma$phage promoter and operator

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Production and Purification of $\gamma$ Phage $cI_{857}$ Repressor Protein by the Use of a Runaway Replication Plasmid Vector (Runaway Replication Plasmid를 이용한$\gamma$Phage $cI_{857}$ Repressor 단백질의 생산 및 정제)

  • 강상모;박인숙
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.122-128
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    • 1992

  • Runaway replication plasmid pSY35AT was used for the production of $cI_{857}$ repressor protein. E, coli MC1065 having plasmid pSY35AT was shifted from $30^{\circ}C$ to $37^{\circ}C$ $cI_{857}$ repressor protein produced was purified by a modification of the purification method of wild type cI repressor. The concentration of purified $cI_{857}$ repressor protein was 0.11 mg/ml. The binding assay of $cI_{857}$ repressor and right promoter - right operater ($P_RO_R$) labeled with $^3H-CTP$ was done. Relative activity of purified cIx5, repressor was higher about 23 times than that of cell free extract. A higher value of the temperature in the binding assay led to a lower value of the binding activity.

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