• 제목/요약/키워드: $\gamma$-Irradiation

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ICR 마우스에서 감마선 조사로 유도된 조혈조직 손상 모델 확립 (Establishment of γ-irradiation-induced Hematopoietic Tissue Damage Model in ICR Mice)

  • 강정애;노종국;장범수;정영진;박상현
    • 방사선산업학회지
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    • 제7권1호
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    • pp.55-59
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    • 2013
  • Ionizing radiation causes the massive generation of reactive oxygen species, resulting in cellular and tissue damage. The present study was performed to evaluate ${\gamma}$-irradiation induced cellular damage in ICR mice. The mice were divided into four groups with ten mice in each group. Group 1 served as an unexposed control group. Groups 2, 3, and 4 were exposed to 3, 5, and 7 Gy of ${\gamma}$-radiation, respectively. Five mice per group were sacrificed 1 and 7 days after ${\gamma}$-radiation. Exposure to ${\gamma}$-irradiation resulted in hematopoietic damage in a dose-dependent manner when compared with the unexposed control group, which featured a significantly decreased spleen index. However, the exposed mice showed no significant differences in their serum AST, ALT and in the histopathological change of their liver. These results suggest that ${\gamma}$-irradiation is a good tool to prepare a hematopoietic damage model. This animal model can be employed to study the hematopoietic efficacy of biologically active compounds.

Differential Modulation of Volatile Constituents in Artemisia princeps and Artemisia argyi Plants after Gamma Ray or Electron Beam Irradiation

  • Kim, Ji Hong;Cho, Eun Ju;Lee, Min Hee;Kim, Ji Eun;Chung, Byung Yeoup;Kim, Tae Hoon;Kim, Jin-Hong
    • 방사선산업학회지
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    • 제6권1호
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    • pp.17-21
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    • 2012
  • The effects of gamma ray or electron beam irradiation on herbaceous medicinal plants were investigated in terms of the composition of volatile constituents using the aerial parts or leaves of Artemisia princeps Pamp. cv. Ganghwayakssuk and Artemisia argyi cvs. Namhaeyakssuk and Hwanghaessuk. The composition of volatile constituents in leaves was clearly distinguishable among the three Artemisia cultivars. However, the relative proportions of the major volatile constituents such as 1,8-cineole, ${\alpha}$-pinene, camphene, santolina triene, and artemesia triene, were similarly changed in two or three cultivars by gamma ray or electron beam irradiation. In particular, the proportion of 1,8-cineole was increased up to 1.29- to 1.71-fold in the three cultivars after irradiation with gamma ray. These results suggest that gamma ray or electron beam irradiation can be applied to modulate the composition of volatile constituents in the leaves of Artemisia plants.

저선량 감마선 반복조사의 육류와 생선 중 미생물 저감효과 (Reducing Effect of Microorganism on Meat and Fish Products by Repeated γ-Irradiation at Low Dose)

  • 제길수;정덕화;심원보
    • 한국식품위생안전성학회지
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    • 제30권1호
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    • pp.92-97
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    • 2015
  • 본 연구에서는 포장방법 (진공, 일반)과 저장온도($4^{\circ}C$, $-20^{\circ}C$)를 달리한 육류 (소고기, 닭고기) 및 생선에 대해 저선량 감마선 반복조사를 적용하였을 때 나타나는 병원성 미생물 E. coli O157:H7 및 S. Typhimurium의 사멸효과를 확인하였다. 소고기와 닭고기의 경우 E. coli O157:H7은 최소 2 kGy 이상의 단일조사와 0.5 kGy 이상으로 반복조사 시 생육이 확인되지 않았고, S. Typhimurium은 최소 2 kGy 이상의 단일조사와 1 kGy 이상으로 반복조사 시 생육이 확인되지 않았다. 생선의 경우 최소 0.5 kGy 이상의 단일 및 반복조사 시 E. coli O157:H7 와 S. Typhimurium의 생육이 확인되지 않았다. 육류보다 생선이 낮은 선량으로 미생물을 사멸시킬 수 있었으며, 전반적으로 포장 방법에 따른 조사선량의 차이는 없었으나 저장온도의 경우 냉장보다 냉동의 경우가 좀 더 높은 조사선량을 요구하는 것으로 확인되었다.

조리 방법별 즉석 취식 닭고기의 조리 직후 감마선 조사가 관능적 품질에 미치는 영향 (Effects of Sensory Qualities of Ready-to-Eat Chicken Cooked by Different Culinary Methods and Gamma-irradiated at 10 kGy)

  • 박경숙
    • 방사선산업학회지
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    • 제6권4호
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    • pp.311-316
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    • 2012
  • The purpose of this study was to compare the sensory effects of different culinary methods such as electric pan cooking and charcoal roasting on gamma irradiated ready-to-eat chicken at 10 kGy. Evlauation of sensory properties were measured by estimating the organoleptic characteristics and physicochemical properties (TBARS, hardness and color different matters). The results showed that gamma irradiation after charcoal-roasting could more effectively moderate the decrease of sensory qualities and reduce off-flavor of ready-to-eat chicken than gamma irradiation after following electric pan cooking. These results suggest that gamma irradiation after charcoal broiling can be an effective culinary method for the preparation of ready-to-eat chicken.

감마선 조사가 휴민산 염소화에 의한 THMs 생성능 및 수질특성에 미치는 영향 (Effect of γ-ray Irradiation on THMs Formation and Water Quality Characteristics in the Chlorination of Humic Acid Contaming Water)

  • 강철호;임현우;정성운;최종혁;김종훈;최용욱;이면주
    • 한국물환경학회지
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    • 제26권6호
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    • pp.1034-1039
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    • 2010
  • Variation of formation potential of THM (THMFP) by chlorination of humic acid and characteristics of water quality with ${\gamma}$-ray irradiation were investigated, which were divided into two categories by the order of ${\gamma}$-ray irradiation and chlorination in water treatment process. The group A consisted of the ${\gamma}$-ray irradiation followed by chlorination process of humic acid, and the group B consisted of the chlorination followed by ${\gamma}$-ray irradiation process. The pH, ORP, $UV_{254}$, and DOC decreased rapidly with an increase in ${\gamma}$-ray irradiation of 3 kGy. while conductivity was little changed. Maximum degradation ratio of chloroform in THMs of group A was 82%, while that in group B was 69%. No brominated THMs were detected at high irradiation (>3 kGy). We found that group A water treatment process was more effective in lowering the THMFP than that of group B.

감마선 조사 방법에 따른 정형외과용 초고분자량 폴리에틸렌의 미세구조 변화 (Microstructural Changes in Orthopaedic-Grade Ultra High Molecular Weight Polyethylene (UHMWPE) according to Gamma-Irradiation Method)

  • 이권용
    • 폴리머
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    • 제34권5호
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    • pp.454-458
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    • 2010
  • 본 연구에서는 인공관절 베어링 부품으로 가장 널리 사용되는 초고분자량 폴리에틸렌(UHMWPE)을 대상으로 6가지 감마선 조사 방법에 따른 UHMWPE의 미세구조 변화를 비교 분석하였다. 감마선을 조사하지 않은 폴리에틸렌(UGI)과 비교하여, 기존의 멸균처리에 해당하는 공기 중 상온에서 감마선 조사한 폴리에틸렌(AR)의 결정화도와 가교 정도는 유의한 차이를 보이며 증가하였다. 감마선 조사 환경 중, 상온에서의 산소(AR)와 진공상태(VR) 영향을 비교하면, AR과 VR의 결정화도는 유의한 차이가 없었으며, VR의 가교정도는 유의한 차이를 보이며 증가하였다. 감마선 조사 환경 중, 진공상태에서의 상온(VR)과 극저온(V77) 영향을 비교하면, 결정화도와 가교정도 모두 유의한 차이가 없었다. 그러나, VR과 V77에서의 가교정도는 AR에서의 가교정도보다 유의한 차이를 보이며 증가하였다. 3가지 감마선 조사 환경에 대하여 감마선 조사 이후 열처리(/S) 영향을 비교하면, 결정화도는 AR/S와 VR/S에서 유의한 차이가 없었으나, V77/S에서는 유의한 차이를 보이며 감소하였다. 또한, 가교정도는 AR/S와 V77/S에서 유의한 차이를 보이며 증가하였으며, VR/S에서는 유의한 차이가 없었다.

방사선 완·급조사에 따른 벼의 생리적 영향 평가 (Physiological Responses of Rice by Acute and Chronic Gamma Irradiation)

  • 김동섭;송미라;김선희;장덕수;강시용;김진백;김상훈;하보근
    • 방사선산업학회지
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    • 제5권1호
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    • pp.55-62
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    • 2011
  • We investigated the physiological responses by acute and chronic gamma-irradiation in rice. The rice, Oryza sativa L. cv Dongan, plants were irradiated with 100 and 400 Gy of gamma-rays for acute and chronic irradiation, and their morphological, chlorophyll content, MDA, proline and activities of antioxidant enzymes were examined. The plant height of chronic irradiation samples were decrease with increase of a does than the control, but the number of tiller and dry weight of shoot were increased 100 Gy. Carotenoid and chrolorphyll content were decreased of all irradiated plants than non-irradiated plants. But, MDA and proline content were increased in 400 Gy both acute and chronic gamma irradiation. And the activities of antioxidant enzymes were different as gamma-irradiation patterns.

감마선 조사가 한우육의 색소에 미치는 영향 (Effects of Gamma Irradiation on Pigments of Beef)

  • 육홍선;이주운;이경행;김성;변명우
    • 한국식품과학회지
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    • 제30권5호
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    • pp.1184-1188
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    • 1998
  • 한우육의 사태(M.Semitendinosus)를 대상으로 방사선 조사선량(0, 1, 3, 5 kGy), 포장방법(함기, 진공) 및 저장기간에 따른 육색소(myoglobin, oxymyoglobin, metmyoglobin)와 Hunter값의 변화를 측정하였다. Hunter값의 경우 저장기간이 경과함에 따라 명도와 황색도는 증가하는 경향을 나타내었으나, 적색도는 감소하였고, 감마선 조사에 의한 영향으로는 명도, 황색도, 적색도값이 조사선량이 증가함에 따라 감소하였다. Myoglobin의 함량은 저장기간이 경과할수록 모든 시험군에서 감소를 나타내었고, oxymyoglobin과 metmyoglobin은 점차적으로 증가하였으며, 방사선 조사에 따른 영향은 조사선량이 증가함에 따라 myoglobin은 감소되고 oxymyoglobin과 metmyoglobin은 다소 증가하는 경향을 나타내었다. 포장방법에 따른 영향에서는 함기 포장군이 진공포장군에 비해 더 큰 육색소의 변화를 나타내었다.

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Effect of Electron Beam and ${\gamma}$-Ray Irradiation on the Curing of Epoxy Resin

  • Kang, Phil-Hyun;Park, Jong-Seok;Nho, Young-Chang
    • Macromolecular Research
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    • 제10권6호
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    • pp.332-338
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    • 2002
  • The effect of an electron beam and ${\gamma}$-ray irradiation on the curing of epoxy resins was investigated. Diglycidyl ether of bisphenol A (DGEBA) and diglycidyl ether of bisphenol F (DGEBF) as epoxy resin were used. The epoxy resins containing 1.0-3.() wt% of triarylsulphonium hexafluoroantimonate(TASHFA) and triarylsulphonium hexafluorophosphate(TASHFP) as initiator were irradiated under nitrogen at room temperature with different dosage of EB and ${\gamma}$-rays from a Co$^{60}$ u source. The chemical and mechanical characteristics of irradiated epoxy resins were compared after curing of EB and ${\gamma}$-ray irradiation. The thermal properties of cured epoxy were investigated using dynamic mechanical thermal analysis. The chemical structures of cured epoxy were characterized using near infrared spectroscopy. Mechanical properties such as flexural strength, modulus were measured. The gel fraction of DGEBA with ${\gamma}$-ray was higher than that of the epoxy with EB at the same dose. Young's modulus of the sample irradiated by ${\gamma}$-ray is higher than that of sample cured by EB. From the result of strain at yield, it was found that the epoxy cured by ${\gamma}$-ray had a higher stiff property compared with the irradiated by EB.

Effects of ${\gamma}$-Irradiation on Immunological Activities of ${\beta}$-Glucan

  • Kim, Jae-Hun;Sung, Nak-Yun;Byun, Eui-Hong;Kwon, Sun-Kyu;Song, Beom-Seok;Choi, Jong-Il;Yoon, Yohan;Kim, Jin-Kyu;Byun, Myung-Woo;Lee, Ju-Woon
    • Food Science and Biotechnology
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    • 제18권5호
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    • pp.1305-1309
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    • 2009
  • This study evaluated the effects of $\gamma$-irradiation on immunomodulating properties and structural changes of ${\beta}$-glucan. ${\beta}$-Glucan solutions (10 mg/mL) were ${\gamma}$-irradiated at 10, 30, and 50 kGy. Splenocyte proliferation and cytokine (interferon-${\gamma}$ and interlukin-2) productions by ${\gamma}$-irradiated ${\beta}$-glucan were evaluated in in vivo and in vitro, and structural changes of ${\beta}$-glucan were also determined after ${\gamma}$-irradiation. ${\gamma}$-Irradiation on ${\beta}$-glucan at 50 kGy enhanced splenocyte proliferation and cytokine productions, (p<0.05) and cleft glycosidic bonds of ${\beta}$-glucan resulting in lower the molecular weight. These results indicate that the use of ${\gamma}$-irradiation on ${\beta}$-glucan may be useful for improving its immunological activity by lowering the molecular weight of ${\beta}$-glucan.