• Title/Summary/Keyword: $\gamma$-Irradiation

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Changes of Physicochemical and Microbiological Properties of Gamma-Irradiated Miscellaneous Cereals (감마선 조사한 잡곡류의 물리화학적, 미생물적 특성 변화)

  • 손인숙;김미라
    • Journal of the East Asian Society of Dietary Life
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    • v.8 no.4
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    • pp.412-421
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    • 1998
  • Physicochemical and microbiological properties of red beans, soybeans, barleys, and corns irradiated by gamma-ray at 1.2 kGy, 10.1 kGy, or 30.5 kGy were investigated. Moisture content and crude lipid content of the irradiated cereals showed no significant difference from the nonirradiated group. TBA values of corns irradiated at 30.5 kGy increased. Gamma irradiation reduced the numbers of mesophilic and psychrotrophic bacteria in the cereals. Yeasts and molds were reduced below detection levels even at 1.2 kGy dose-irradiation. There was no significant difference in reducing sugars between the irradiated cereals and the nonirradiated ones except corns. Stachyose, raffinose, and sucrose of soy beans and sucrose of barleys increased by 30.5 kGy dose-irradiation. Loss of riboflavin content of the irradiated samples was not observed except red beans irradiated at 30.5 kGy. In Hunter's color, b value of the red bean powder increased but L value of the soybean powder decreased by the Irradiation. L, a, and b values of the barley powder slightly increased under 30.5 kGy dose-irradiation and b value of the corn powder decreased under 10.1 or 30.5 kGy-dose irradiation.

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Effects of Gamma Irradiation on the Decontamination of Animal Feeds: Sterilization of Carbohydrate Sources (배합사료 원료에 대한 방사선 살균 효과 ; 탄수화물의 살균)

  • 조한옥;변명우;권중호;이재원;김영배
    • Journal of Food Hygiene and Safety
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    • v.2 no.3
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    • pp.97-102
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    • 1987
  • The effects of gamma irradiation on microbiological and physicochemical qualities in raw ingredients (thirteen kinds of cereal grain and their by-product) of mixed feeds were investigated. The total aerobic bacteria counts in the samples were $10^2\;to\;10^6/g$. They were sterlllzed to a undetectable level by 5 to 7 kGy irradiation. Coliforms were contaminated in high levels in all sample, ranging from $10^2\;to\;10^6/g$. They were radiation-llensitive and completely eliminated by irradiation with 3 to 5 kGy. Total fungi, ranging from $10^2\;to\;10^4/g$, mainly osmophiles were identified as Aspergillus and Penicillium. They were eliminated below identification limit by 5 to 7 kGy irradiation. Seven kinds of species, including Aspergillus IkrlJUB. were identified as a potential mycotoxin producers. Physicochemical qualities, such as total amino acid content, total sugar content. TBA value and color difference showed that an optimum dose of irradiation was less detrimental than ethylene oxide fumigation.

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Kojic Acid Protects C57BL/6 Mice from Gamma-irradiation Induced Damage

  • Wang, Kai;Liu, Chao;Di, Chan-Juan;Ma, Cong;Han, Chun-Guang;Yuan, Mei-Ru;Li, Peng-Fei;Li, Lu;Liu, Yong-Xue
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.1
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    • pp.291-297
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    • 2014
  • The radioprotective effects of a single administration of kojic acid (KA) against ionizing radiation were evaluated via assessment of 30-day survival and alterations of peripheral blood parameters of adult C57BL/6 male mice. The 30-day survival rate of mice pretreated with KA (75 or 300 mg/kg body weight, KA75 or KA300) subcutaneously 27 h prior to a lethal dose (8 Gy, 153.52 cGy/min) of gamma irradiation was higher than that of mice irradiated alone (40% or 60% vs 0%). It was observed that the white blood cell (WBC) count/the red blood cell (RBC) count, haemoglobin content, haematocrit and platelet count of mice with or without KA pretreatment as exposed to a sub-lethal dose (4 Gy, 148.14 cGy/min) of gamma irradiation decreased maximally at day 4/day 8 post-irradiation. Although the initial WBC values were low in KA300 or WR-2721 (amifostine) groups, they significantly recovered to normal at day 19, whereas in the control group they did not. The results from the cytotoxicity and cell viability assays demonstrated that KA could highly protect Chinese hamster ovary (CHO) cells against ionizing radiation with low toxicity. In summary, KA provides marked radioprotective effects both in vivo and in vitro.

Effects of Gamma Irradiation on Physicochemical Properties of Red Beet and Stability of Betalain in the Red Beet (Beta vulgaris L.) (감마선 조사가 비트(Beta vulgaris L.)의 물리화학적 특성 및 Betalain 색소 안정성에 미치는 영향)

  • Kim, Kyoung-Hee;Lee, Sung-A;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.4
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    • pp.453-457
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    • 2007
  • The physicochemical properties of red beets and the stability of betalain pigments irradiated at 2.5, 5, 10 and 30 kGy were evaluated. Betalain extraction yield of irradiated red beets was not significantly different from non-irradiated red beet. The red beet irradiated at 5kGy showed high optical density value in betacyanin and betaxanthin. In Hunter's color value lightness (L), redness (a) and yellowness (b) decreased with increment of irradiation dose. All red beets showed no difference in pH. The red beets irradiated above 10 kGy had poor textural property quality but antioxidant activity of betalain was not significantly changed by gamma irradiation. Considering natural colorant and textural quality, the gamma irradiation above 10kGy was an undesirable technique for red beets.

Dose Determination in the IR-221 Gamma Facility Using a Monte Carlo Simulation (몬테칼로 시뮬레이션을 이용한 IR-221의 선량 평가)

  • Lim, Ik-Sung;Kim, Ki-Yup;Roh, Gyu-Hong;Lee, Chung
    • Journal of Radiation Protection and Research
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    • v.32 no.1
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    • pp.21-26
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    • 2007
  • This study is performed to evaluate the dose rate and to analyze the dose distribution of the gamma irradiation facility (IR-221) by using a Monte Calro simulation, which is helpful of upgrading the radiation processing qualification. Monte Cairo simulation is performed by MCNP4B code. Dose rates were measured at total 369 points with alanine dosimeters to compare the calculation results and the measurements data. The results have shown that the MCNP4B code is very useful to determine the dose distribution of the IR-221 gamma irradiation facility, as the calculation dose rate is within about ${\pm}5%$ of the measurement data. Dosimetry about the gamma irradiation facility usually needs enormous manpower and time. However Monte Cairo calculation method can reduce the tedious dosimetry jobs and improve the irradiation processing qualification, which will probably contribute to obtain the reliability of the irradiation products.

Effects of Gamma Irradiation on Changes of Chemical Compounds in the Processing of Fermented Shrimp with Law Salt (감마선 이용 저염 새우젓 제조시 화학성분의 변화)

  • 안현주;이경행;이철호;차보숙;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.4
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    • pp.629-634
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    • 2000
  • The effects of gamma irradiation on changes of chemical compounds of fermented shromp with low salt were studied. The shirmp was salted NaCl concentration of 10%, 15% and 20%, and irradiated at 0, 2.5, 5.0, 7.5 and 10.0kGy. Amino nitrogen (AN), volatile basic nitrogen (VBN), trimethylamine (TMA) and neutral protease acivity were examined during ferentation at 15$^{\circ}C$. A sample with 30% salt concontration was also prepared as a control. The initial contents of AAN, VBN, TMA and protease acitivity were not affected by gamma irradiation. The contents of AN, VBN and TMA were incteased with fermentation period. But, the more increased NaCl concentrations and the higher irradiation dose, the loss increased content of chemical compounds and protease activity were found. Protease was increased until 4~5 weeks and then decreased gradually. The results showed that the chemical compounds and protease activity of salted abd fermeted shrimp prepared with 15% NaCl contentration and 10 kGy irradiation dose, or 20% and 5 kGy or higher were maintained the appropriate level of quality up to 10 weeks of storage compared with the control.

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Microbiological Quality Stability of Fresh mushroom (Agaricus bisporus) by Gamma Irradiation (감마선조사에 의한 양송이버섯의 미생물적 품질안정)

  • 변명우;권중호;조한옥;차보숙;강세식;김중만
    • Journal of Food Hygiene and Safety
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    • v.5 no.1
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    • pp.21-26
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    • 1990
  • The effect of gamma irradiation on microbiological quality of fresh mushrooms (Agricus bisporus) were investigated. Gamma irradiation was found to be effective in lowering microbial counts initially and throughout storage. Decreases in microbial counts were strongly correlated with doses initially and during storage. Microbial counts of 2 to 3 kGy irradiated mushrooms remained significantly lower than unirradiated control mushrooms for a period of up to 2 to 3 weeks when stored at $9\;{\pm}\;1^{\circ}C\;and\;80\;{\pm}\;7%$ RH. Therefore, qualitative and quantitative changes in mushroom microflora by irradiation contributed to overall increases in shelf life of fresh mushrooms.

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Effects of Ethylene Oxide Fumigation and Gamma Irradiation on the Quality of Dried Agricultural Products (Ethylene Oxide 처리와 Gamma가 조사가 건조 농산물의 품질에 미치는 영향)

  • 조한옥;권중호;변명우;양재승;김영재
    • Journal of Food Hygiene and Safety
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    • v.1 no.2
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    • pp.133-141
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    • 1986
  • Gamma irradiation as a new physical treatment was applied to comparative researches with a conventional chemical fumigant on the microbiologicai and physicochemical qualities of selected agricultural products such as powdered hot pepper soybean paste,. oyste.mushroom powder, carrot flake, and raw sesame. The microorganisms contaminated in the sample, including total bacteria, thermophiles, acid tolerant bacteria, fungi, osmophilic molds and coliforms were sterilized with irradiation doses of 7-10 kGy, while ethylene oxide (E.O) fumigation proved insufficient for the destruction of them. An optimum dose of irradiation was less detrimental than E.O. fumigation to the physicochemical properties of the sample. Sensory evaluation after three months of storage at room temperatures showed that the overall acceptability of irradiated sample was higher than that of the non treated control as well as E.O. fumigated samples.

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Improvement of the Hygienic Quality and Shelf-life of Kwamegi from Cololabis seira by Gamma Irradiation (꽁치과메기의 위생적 품질개선 및 저장기간 연장을 위한 감마선 조사)

  • Cho, Kyung-Hwan;Lee, Ju-Woon;Kim, Jae-Hun;Ryu, Gi-Hyung;Yook, Hong-Sun;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1102-1106
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    • 2000
  • This study was carried out to investigate the effects of gamma irradiation for the improvement of hygienic quality and the extension of shelf-life of Kwamegi(semi-dried colobabis seira). Kwamegi was stored at $5^{\circ}C$ and $15^{\circ}C$ after gamma irradiation with doses of 0, 3, 5, 7 and 10 kGy. In microbiological aspects, non-irradiated Kwamegi was rapidly deteriorated during storage, and some harmful bacteria were detected in a microbial analysis using a selective medium. However, the total viable cells and presumptive pathogens were reduced with the increase of irradiation dose, and dose level of 7 to 10 kGy was considered to be optimum and effective dose for the preservation of Kwamegi.

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Selection of Male-sterile and Dwarfism Genetically Modified Zoysia japonica through Gamma Irradiation (감마선 처리에 의한 웅성불임 및 왜성형질의 유전자변형 들잔디(Zoysia japonica Steud.) 선발)

  • Bae, Tae-Woong;Song, In-Ja;Kang, Hong-Gyu;Jeong, Ok-Cheol;Sun, Hyeon-Jin;Ko, Suk-Min;Lim, Pyung-Ok;Song, Pill-Soon;Song, Sung Jun;Lee, Hyo-Yeon
    • Journal of Radiation Industry
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    • v.4 no.3
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    • pp.239-246
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    • 2010
  • The aim of this study is selection of the male-sterile plant for inhibiting transgene flow through gamma-irradiation ($^{60}Co$) at the pollination and fertilization cycle of herbicide-tolerant genetically modified (GM) zoysiagrass (Zoysia japonica Steud.). High frequencies of plant mutations were obtained about 18% from $M_1$ generation at the doses (10 to 50 Gy). We also found that some $M_1$ plants showed male-sterile plants using de-husked seeds and comparison of stainable pollen using $KI-I_2$ solution. Besides the effects of irradiation on pollination and fertilization cycle, various other mutations like dwarf, cold tolerance, increasing grains and mass were observed. Four of dwarfism plants were selected through comparison of morphological characteristic between control and mutants during 4 years. These results demonstrated that the gamma-irradiation on pollination and fertilization cycle is very effective to induce the various mutations, and the male-sterile mutants are useful for controlling transgene flow and developing of high quality turfgasses.