• 제목/요약/키워드: $\gamma$-Irradiation

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방사선에 의한 키토산을 포함하는 PVA와 PVP 하이드로겔의 제조 및 특성 (Preparation and Characterization of Hydrogels of PVA and PVP Containing Chitosan by Radiation)

  • 박경란;노영창
    • 폴리머
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    • 제25권5호
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    • pp.728-735
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    • 2001
  • 본 연구에서는, $^{60}cO$ 방사선 (${\gamma}$-rays) 가교를 이용하여 PVA/키토산과 PVP/키토산의 혼합물로부터 하이드로겔을 제조하였다. 하이드로겔이 상처 치료용 드레싱으로 사용될 수 있는지 예측하기 위해 겔화율, 팽윤도, 겔강도같은 기계적 성질을 측정하였다. PVA와 키토산 및 PVP와 키토산의 비는 97 : 3 ~ 90 : 10이고, PVA/키토산 및 PVP/키토산 용액의 고형분의 농도는 15wt% 이었다. 하이드로겔의 기계적 성질에 조사선량이 미치는 영향을 예측하기 위해 PVA/키토산 및 PVP/키토산 혼합물에 25~70kGy의 감마선을 조사하였다. 겔화율과 겔강도는 키토산 조성비가 작을수록, 조사선량이 커질수록 증가하였다 팽윤도는 키토산 조성비가 클수록, 조사선량이 작을수록 증가하였다.

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감마선조사에 의한 미역 추출물 항염증제제의 갈색 색상 제거 (Gamma-irradiation Elimination of Brown Color from the Anti-inflammatory Agent Containing an Undaria pinnatifida Extract)

  • 강지영;김아람;송유진;박진규;이주운;변명우;안동현;홍용기
    • 한국수산과학회지
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    • 제42권5호
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    • pp.468-475
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    • 2009
  • This study was conducted to examine the bleaching of an anti-inflammatory agent containing Undaria pinnatifida extract (AIAU). Brown-colored AIAU was irradiated with $^{60}CO$ gamma rays at doses ranging from 10-200 kGy. Discoloring of the anti-inflammatory agent was achieved by gamma irradiation, with significantly removal by 50 kGy irradiation. The main active compound of the agent consisted of 4% U. pinnatifida extract, which inhibited inflammatory symptoms in mouse ear edema by 93%. There was no change in effects against edema, erythema, blood flow and radical scavenging activity by irradiation.

Effect of Irradiation of Red Radish Seeds on the Seed Viability and Functional Properties of Sprouts

  • Waje, Catherine K.;Park, Ju-Hwan;Kim, Gui-Ran;Kim, Yu-Ri;Han, Bum-Soo;Lee, Yeon-Kyung;Moon, Kwang-Deog;Kwon, Joong-Ho
    • Food Science and Biotechnology
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    • 제18권2호
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    • pp.390-395
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    • 2009
  • Red radish seeds were irradiated at doses up to 8 kGy using electron beam (e-beam) and gamma ray ($\gamma$-ray). The seed viability and functional properties (carotenoid, chlorophyll, ascorbic acid, and total phenol) of sprouts grown from these irradiated seeds were evaluated. High germination percentage ($\geq$97%) was observed in seeds irradiated at $\leq$5 kGy, but the yield ratio and sprout length significantly decreased with increased irradiation dose. Irradiation at $\geq$6 kGy resulted in curling of the sprout roots. Sprouting enhanced the functional properties of red radish seeds as indicated by the increased carotenoid, chlorophyll, ascorbic acid, and total phenol contents during germination. However, radiation treatment hampered the growth of seeds resulting in underdeveloped sprouts with decreased carotenoid, chlorophyll, ascorbic acid, and total phenol contents. In general, e-beam and $\gamma$-ray irradiation of red radish seeds showed similar effects on the seed viability and functional properties of sprouts. Postharvest storage reduced the functional quality of sprouts.

염장미역의 감마선 조사에 따른 이화확적 특성 변화 (Changes in Physico-Chemical Properties of Salted Sea Mustard (Undaria Pinnatifida) by Gamma Irradiation)

  • 변명우;권중호;이수정;남상명;조한옥
    • 한국식품위생안전성학회지
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    • 제6권3호
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    • pp.165-169
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    • 1991
  • 염장미역 제품의 염농도 감소와 저장 조건 개선을 위한 연구의 일환으로 감마선 조사가 염장미역의 이화학적 특성에 미치는 영향을 조사한 결과는 다음과 같다. 미역의 chlorophyll과 carotenoid 색소는 감선 조사에 의해 부분적으로 감소되었고 $10^{\circ}C$ 내외의 저장조건에서 6개원 저장시 비조사 시료와 2kGy 조사시료의 색소 잔존률은 서리 유의적인 차이가 없이 chlorophyll이 47~77%, carotenoid가 54~56%였다. 미역의 관능적 품질과 관련된 유기산과 휘발산의 함량은 염농도가 낮고 저장온도가 높을수록 증가하였고, 감마선 조사에 의한 미역의 조직 연화는 알긴산 추출과 관련이 있는 것으로 나타났다.

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Application of Gamma Irradiation for the Reduction of Residual Nitrite and Nitrosamine in Meat Products

  • Ahn, Hyun-Joo;Kim, Jae-Hun;Lee, Ju-Woon;Jo, Cheorun;Byun, Myung-Woo
    • 한국축산식품학회지
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    • 제24권4호
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    • pp.367-372
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    • 2004
  • Nitrite, a curing agent of meat products, is precursors of carcinogenic N-nitrosamines during processing of meat products or under human stomach conditions as well as having its own toxicity. Some researches have been conducted to evaluate the effects of ionizing radiation on the reduction of residual nitrite and N-nitrosamines in an aqueous model system and cured meat products with different packaging methods during storage. These results showed that the gamma irradiation was effective in reducing the residual nitrite and N-nitrosamines in an aqueous model system as well as meat products. Especially, irradiation combined with vacuum or modified atmosphere packaging was more effective in nitrite and N-nitrosamines reduction than aerobic packaging during storage. The objective of this review is to introduce the irradiation technology for the application of reducing the residual nitrite and N-nitrosamine contents in meat products.

Synthesis and Biocompatibility of PVA/NaCMC Hydrogels Crosslinked by Cyclic Freezing/thawing and Subsequent Gamma-ray Irradiation

  • Shin, Ji-Yeon;Jeong, Heeseok;Lee, Deuk Yong
    • 대한의용생체공학회:의공학회지
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    • 제39권4호
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    • pp.161-167
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    • 2018
  • Polyvinyl alcohol/sodium carboxymethyl cellulose (PVA/NaCMC) hydrogels were prepared by physical crosslinking (cyclic freezing/thawing) and gamma (${\gamma}$)-ray irradiation to evaluate the effect of NaCMC concentration (2~8 wt%) on the mechanical properties and the biocompatibility of the PVA/NaCMC hydrogels. The swelling rate of PVA/NaCMC hydrogels regardless of irradiation rose with increasing NaCMC content from 2 wt% to 8 wt%, while the gelation rate was the reverse. As the NaCMC content increased from 2 wt% to 6 wt%, the compressive strength of the hydrogels increased dramatically from $8.5{\pm}2.0kPa$ to $52.7{\pm}2.5kPa$ before irradiation and from $13.5{\pm}2.9kPa$ to $65.5{\pm}8.7kPa$ after irradiation. When 8 wt% NaCMC was added afterwards, the compressive strength decreased however. The irradiated PVA/NaCMC hydrogels containing 6 wt% NaCMC exhibited the tailored properties of the swelling rate of $118{\pm}3.7%$, the gelation rate of $71.4{\pm}1.3%$, the strength of $65.5{\pm}8.7kPa$, respectively, and no cytotoxicity was observed.

Enzymatic Degradation of Poly(${\gamma}$-glutamic acid) Hydrogel Prepared by ${\gamma}$-Ray Irradiation

  • Hara, Toshio;Choi, Seong-Hyun;Choi, Woo-Young
    • Journal of Microbiology and Biotechnology
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    • 제11권2호
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    • pp.342-345
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    • 2001
  • A bacterial strain PH-4, which produces an enzyme catalyzing the degradation of crosslinked poly(${\gamma}$-glutamic acid) hydrogels, was isolated and identified as a Flavobacterium sp. The enzyme was obtained by the sonication of the bacterial cells preincubated in a Bouillon medium with shaking, without adding of poly(${\gamma}$-glutamic acid) as an inducer. The products of the hydrogel degraded by the crude enzyme agreed closely with the depolymerized materials in SDS-polyacrylamide gel electrophoresis using methylene blue staining, and with a glutamic acid monomer on thin-layer chromatography, thereby suggesting that strain PH-4 produced a kind of exohydrolase.

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감마선을 조사한 桃仁皮(도인피)의 항산화활성 (Anti-oxidative Activities Gamma Irradiated Shell of Persicae semen)

  • 조우아
    • 대한본초학회지
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    • 제24권4호
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    • pp.95-99
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    • 2009
  • Objectives : This study were carried out to investigate the anti-oxidative activities of $\gamma$-SPE(gamma irradiated shell of Persicae semen extract with ethanol). Methods : Anti-oxidative was measured by DPPH free radical scavenging activities, nitric oxide radical scavenging activities, SOD-like activities, xanthine oxidase radical scavenging activities, nitrite scavenging activities and tyrosinase inhibition activities. Results : Effects of free radial scavenging was increased by irradiation. Tyrosinase inhibition activities was decreased by $\gamma$-SPE. Conclusions : Results suggested that $\gamma$-SPE have a potential as a new anti-oxidative material.

Confirmatory test of gamma irradiation against the larvae and pupae of Helicoverpa assulta (Lepidoptera: Noctuidae) in paprika

  • Park, Jeong Sun;Jeong, Su Yeon;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • 제31권2호
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    • pp.103-106
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    • 2015
  • The oriental tobacco budworm, Helicoverpa assulta (Lepidoptera: Noctuidae), is classified as a quarantine pest that must be controlled for the exportation of Korean paprika to the USA. We performed a confirmatory test of gamma irradiation against the last-instar larvae and pupae of H. assulta in paprika. Previous, small-scale gamma irradiation at a dose of 100 Gy to the last-instar larvae allowed emergence of adults, though they were abnormal, but 200 Gy prevented adult emergence completely. For pupae, irradiation of 5- to 6-day-old pupae with 300 Gy prevented normal emergence completely. To gather confirmatory data applicable to phytosanitary quarantine regulations, larvae and pupae were placed inside paprika in a box and were irradiated with 200 Gy and 300 Gy, respectively. After irradiation with 200 Gy (measured doses 170-199 Gy) of 2,186 individuals of the last-instar larvae, 10.84% survived, but either formed abnormal pupae (7.57%) or died during the pupal stage (3.27%), resulting in no emergence of normal adults. For pupae, the dose of 300 Gy (measured doses 276-319 Gy) given to 1,200 pupae allowed 10.75% to survive and 9.17% to emerge with deformity. However, 1.58% of irradiated pupae emerged normally, requiring an increased dose for complete prevention of normal emergence. Subsequently, an increased dose of 400 Gy (measured doses 340-402 Gy) to 1,005 pupae allowed 88.35% to emerge, but all emerged with deformity. Thus, irradiation treatment with a minimum dose of 400 Gy will provide quarantine security for all premature H. assulta in exported paprika.

Screening of Radio-resistant Lactic Acid Bacteria

  • Hwang, E-Nam;Kang, Sang-Mo;Kim, Jae-Kyung;Lee, Ju-Woon;Park, Jong-Heum
    • 한국축산식품학회지
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    • 제33권3호
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    • pp.335-340
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    • 2013
  • This study screened for radio-resistant strains lactic acid bacteria (LAB) by evaluating their capability to survive exposure to ionizing radiation. Ten strains of LAB - Lactobacillus bulgaricus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbruekii, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium breve, and Pediocuccos pentosaceus - were selected and subcultuted twice. The LAB was then further cultured for 3 d at $37^{\circ}C$ to reach 7-10 Log colony-forming units (CFU)/mL prior to irradiation and immediately exposed to gamma rays or electron beams with absorbed doses of 0, 1, 2, 3, 4, 5, 6, 8, and 10 kGy. Gamma irradiation gradually decreased the number of the tested viable LAB, and the effect was irradiation dose dependent. A similar effect was found in electron beam-irradiated LAB. Radiation sensitivity of LAB was calculated as $D_{10}$ values, which ranged from 0.26 kGy to 0.9 kGy and 0.5 kGy to 1.44 kGy with exposure to gamma and electron beam irradiation, respectively, in all tested LAB. L. acidophilus was the most resistant to gamma and electron beam irradiation, with $D_{10}$ values of 0.9 kGy and 1.44 kGy, respectively. These results suggest that L. acidophilus might be suitable for the preparation of probiotics as direct-fed microbes for astronauts in extreme space environments.