• Title/Summary/Keyword: $\ell$-다양성

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낙동강 중류의 식물플랑크톤상과 군집구조

  • 문성기;최철만
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2000.05a
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    • pp.243-244
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    • 2000
  • 낙동강 중류에서 조사된 식물플랑크톤상은 7강 42속 77종류였고 Bacillariophyceae가 43종류(55.8%), Chlorophyceae 24종류(31.2%), Cyanophyceae 4종류(5.2%), Dinophyceae와 Euglenophyceae이 각각 2종류(2.6%), Chrysophyceae와 Cryptophyceae가 각각 1종류 (1.3%)였다. 주요종은 Stephanodiscus hantzschii를 비롯한 6속 7종류였고 모두 규조류였다. 현존량은 최저 79 cells/$m{\ell}$(3월, 고령)에서 최고 33,023 cells/$m{\ell}$(2월, 물금)로 나타났다. 우점도지수는 최저 0.434(8월, 남지), 최고 0.999(1월, 2월, 물금)였다. 종다양성지수는 최저 0.026(2월, 물금), 최고 3.073(9월, 남지)로 조사되었다.

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남해산 해조류의 항산화 활성

  • 신정혜;주종찬;최덕주;권오천;성낙주
    • Proceedings of the Korean Journal of Food and Nutrition Conference
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    • 2004.07a
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    • pp.45-45
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    • 2004
  • 대사작용의 개선, 정장작용과 유독 물질의 제거, 혈압과 콜레스테롤치의 정상화, 항균 및 항암효과 등 다양한 생리작용을 갖는 것으로 알려진 해조류의 기능성 규명을 위한 연구의 일환으로 총 6종(진두발, 갈래곰보 꼬시래기, 돌가사리, 고장초 톳)의 해조류의 에탄을 추출물에 대한 항산화 효과를 실험하였다. 해조류의 환원력은 시료의 첨가농도에 비례하는 경향이었으며, SOD 유사활성은 고장초의 경우 가장 높은 활성을 나타내었는데 첨가농도 500 $\mu\textrm{g}$/$m\ell$와 1000 $\mu\textrm{g}$/$m\ell$에서 각각 35.43%와 56.71%의 활성을 나타내었다. (중략)

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Inhibition of Cell Adhesion by Noncyclic Triterpenoids from Alpinia katsumadai (초두구로부터 분리한 비환식 트리테르페노이드계 화합물의 세포 접착인자 저해활성)

  • Kim, Ji-Soo;Rho, Mun-Chual;Kim, Gang-Seong;Kim, Koanhoi;Rhim, Byung-Yong
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.4
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    • pp.250-256
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    • 2007
  • The present study purified two noncyclic triterpenoids, compound 1 and compound 2, that intervene interaction of ICAM-1 and LFA-1 from ethanol extracts of Alpinia katsumadai. The compound 1 and 2 inhibited adherence of sICAM-1 to THP-1 cells with an $IC_{50}$ of $7.59{\mu}g/m{\ell}$$6.98{\mu}g/m{\ell}$, respectively, without affecting viability of the cells. The compound 1 and 2 also inhibited interaction of CHO-ICAM-1 cells with THP-1 cells with an $IC_{50}$ $6.7{\mu}g/m{\ell}$$5.5{\mu}g/m{\ell}$, respectively. These findings suggest that the noncyclic triterpenoids from Alpinia katsumadai have inhibitory activities against cell adherent molecules. The present study proposes that noncyclic triterpenoids from Alpinia katsumadai can applied to therapeutic approaches to the diseases that are associated with adhesion of inflammatory cells.

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Ecological Characteristics of Phytoplankton Communities in the Coastal Waters of Gori, Wolseong, Uljin and Younggwang II. Distributions of Standing Crops and Environmental Variables (1992~1996) (고리, 월성, 울진 및 영광 연안해역에서 식물플랑크톤 군집의 생태학적 특성 II. 현존량 분포 및 환경요인들(1992~1996))

  • 강연식;최중기
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.7 no.3
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    • pp.108-128
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    • 2002
  • In order to investigate the ecological characteristics of phytoplankton communities around a nuclear power plant in Gori coastal waters of the South East Sea, Wolseong and Uljin coastal waters of the East Sea and Younggwang coastal waters of the Yellow Sea, the standing crops and chlorophyll-$\alpha$ concentrations of phytoplankton were studied during 1992~1996 and the relationships between standing crops and environmental variables were analyzed. The concentrations of nitrogenous nutrients were on average 0.101, 0.094, 0.072 and 0.108mg/$\ell$ and those of phosphorus were on average 0.007, 0.008, 0.006 and 0.009mg/$\ell$ in Gori, Wolseong, Uljin and Younggwang, respectively. The N:P ratios were highly variable, ranging from 3.2 to 57.3, from 3.1 to 109.0, from 2.6 to 102.0 and from 1.0 to 165.0 in Gori, Wolseong, Uljin and Younggwang, respectively. The concentrations of suspended solids were on average 18.7, 16.7, 11.6 and 52.7mg/$\ell$ and transparencies were on average 3.8, 5.4, 7.9 and 0.7 m in Gori, Wolseong, Uljin and Younggwang, respectively. Total standing crops of phytoplankton averaged 710,659, 687,508, 656,245 and 1,278,173cells/$\ell$ in Gori, Wolseong, Uljin and Yaunggwang, respectively. The standing crops of microplankton(>20${\mu}{\textrm}{m}$) averaged 357,546, 333,638, 276,407 and 592,975cells/$\ell$ those of nanoplankton(<20${\mu}{\textrm}{m}$) averaged 353,113, 353,870, 379,838 and 574,563cells/$\ell$ in Gori, Wolseong, Uljin and Younggwang, respectively. While standing crops of diatoms were averaged 282,009, 284,710, 238,758 and 574,563 cells/$\ell$, those of dinoflagellates were averaged 46,079, 35,401, 32,906 and 16,749 cells/$\ell$ in Gori, Wolseong, Uljin and Younggwang, respectively. The seasonal standing crops of diatoms in Gori, Wolseong and Uljin were higher in Spring than other seasons, but were lower in Summer than other seasons in Younggwang. The seasonal standing crops of dinoflagellates in Gori and Younggwang were higher in Summer than other seasons, but were higher in Autumn than other seasons in U]jin. Average of chlorophyll-$\alpha$ concentrations ranged from 2.16 to 4.28$\mu\textrm{g}$/$\ell$ in 4 study areas with the highest concentration occurred in Younggwang. Indices of species diversity ranged from 2.11 to 2.24 in 4 study areas. While community structures of phytoplankton were unstable during winter and stable during summer in Gori, Wolseong and Uljln coastal waters, those of phytoplankton were stable during winter and summer than during spring and autumn in Yaunggwang. The analysis results of Pearson product moment correlation coefficient between standing crops and environmental variables showed that distributions of standing crops were affected by transparencies, suspended solids, and some nutrient(N $O_3$$^{[-10]}$ -N, P $O_4$$^{3-}$-P), even though the degree of influences were a little different according to the season and the surveyed zone.

Application of Improved Invert in Combining Manhole (합류맨홀에서 개선형 인버트의 적용)

  • Jo, Jun Beom;Lee, Min Sung;Rim, Chang Soo;Yoon, Sei Eui
    • Proceedings of the Korea Water Resources Association Conference
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    • 2018.05a
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    • pp.287-287
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    • 2018
  • 우수관거 시설에서 맨홀은 관거의 접합을 위해 반드시 설치되어야 하는 중요한 요소이다. 이러한 맨홀 접합부에서는 유입관으로 유입되는 유량의 급격한 확대와 유출관으로 배수되는 유량의 급격한 축소 등 흐름의 복잡한 변화가 발생한다. 맨홀에서의 복잡한 흐름은 설계강우를 초과하지 않는 강우량에서 과부하 조건을 형성하며 도심지 침수의 심각한 영향을 미친다. 특히 여러 관이 접합되어 있는 합류맨홀의 경우 유출관거로의 원활한 배수가 유도되지 않을 뿐 아니라 다양한 방향의 흐름이 서로 상충하며 급격한 에너지 손실을 유발한다. 도심지 중 하류부에서 주로 발생하는 침수피해는 복잡한 우수관거 시설의 구성에 따른 합류맨홀의 증가로 인해 그 규모가 늘어나는 추세이다. 바닥이 평평한 기본형태의 맨홀에서 발생되는 과부하 흐름과 여러 유량 조건에서의 에너지 손실에 관한 연구는 지속적으로 수행되어왔다. 최근 맨홀 내부에 흐름을 유도시켜주는 인버트를 설치하여 에너지 손실을 저감하려는 연구가 활발히 이루어지고 있지만 이를 실제 도시지역에 적용하여 침수해석이나 관거 배수능력 평가를 수행하기 위한 필요한 기초자료는 미비한 실정이다. 본 연구에서는 인버트가 설치된 개선형 4방향 합류맨홀의 유량 조건을 다양하게 변화시키며 유입유량 조건에 따른 개선형 맨홀의 손실계수를 분석하였다. 유량 조건은 세 개의 유입관에서 각각 설계유량 $1{\ell}/s$의 유량이 유입되는 총 유입유량 $3{\ell}/s$를 기준으로 각 유입관의 유입유량을 10%씩 변화시킨 40case의 유량 조건을 선정하였다. 이렇게 선정된 유량 조건은 경우에 따라 중간 맨홀, 3방향 합류맨홀 또는 4방향 합류맨홀의 흐름을 다양하게 나타내었으며, 40case의 손실계수를 분석하여 모든 맨홀 접합 조건에서의 손실계수를 파악할 수 있었다. 합류맨홀에서의 개선형 인버트 적용성을 확인하기 위하여 인버트가 설치된 개선형 맨홀에서 산정된 손실계수를 기초로 모든 유입유량 조건에서 적용이 가능한 손실계수 범위도를 작도하였다. 손실계수 범위도는 통계분석에 활용되어 개선형 맨홀의 유입유량 조건에 따른 손실계수 산정식을 도출하는 기초자료로 활용되었다. 이와 같이 도출된 산정식을 적용하면 실제 도심지에 개선형 맨홀을 적용하였을 경우에도 정확한 침수 해석이나 관거 배수능력 평가가 가능할 것으로 판단된다.

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Antifungal and Antioxidant Activities of Extracts from Leaves and Flowers of Camellia japonica L. (동백나무 잎과 꽃 추출물의 항미생물 활성 및 항산화 효과)

  • Lee, Sook-Young;Hwang, Eun-Ju;Kim, Gi-Hae;Choi, Young-Bok;Lim, Chae-Young;Kim, Sun-Min
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.93-100
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    • 2005
  • This research was performed to investigate the possibilities of industrial usage of camellia (Camellia japonica L.) by examining the antioxidant and antimicrobial effects of methanol extract with different sections. Content of total phenolics, DPPH radical scavenging activities and antibacterial activity of young leaf, mature leaf, flower bud, flower, bark, and seed of camellia were compared in vitro experimental models. Total phenolics was contained the higher in young leaf (74.62 mg), flower bud (65.02 mg) and flower (62.42 mg) but less than 20.95 mg per 100 g of dry weight in other parts of Camellia japonica L. And effects of antioxidant measured by DPPH radical scavenger activity ($RC_{50}$, reduce concentration 50%), was shown higher $7.16{\sim}18.14\;{\mu}g/m{\ell}$ in methanol extract of young leaf, flower bud and flower than $61.23\;{\mu}g/m{\ell}$ of BHT as a chemical oxidant. Also, the antimicrobial activity of Camellia japonica L. extracts determined using a paper disc method against food-borne pathogen and food spoilage bacteria, the young leaves extracts showed the most active antimicrobial activity against 7 kinds of harmful microorganisms. Flower bud extracts showed the highest antibacterial activity against P. aeruginosa and Enterobacter spp. C1036. In addition, the minimum inhibitory concentration (MIC) of young leaf extract against B. subtillis,S. fradiae,S. aureus,E. coli,P. aeruginosa, Enterobacter spp. C1036, and S. typhimurium were revealed 1 to 15 ${\mu}g/m{\ell}$. As a result, antimicrobial activity of camellia extracts was shown higher gram positive bacteria than gram negative bacteria.

Biological Activities and Bioactive Compounds in the Extract of Acer tegmentosum Maxim. Stem (산겨릅나무 줄기추출물의 생리활성 및 유효성분 분리)

  • Hong, Bo-Kyong;Eom, Seok-Hyun;Lee, Chan-Ok;Lee, Ji-Won;Jeong, Jong-Hyun;Kim, Jae-Kwang;Cho, Dong-Ha;Yu, Chang-Yeon;Kwon, Yong-Soo;Kim, Myong-Jo
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.4
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    • pp.296-303
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    • 2007
  • Acer tegmentosum (Acereaceae) has been used a source of traditional medicines for the treatment of hepatic disorders in Korea. This research was conducted to determine biofunctional activities of A. tegmentosum stem extract and to identify its bioactive components. Methanolic extract from A. tegmentosum stem was partitioned by using organic solvents, including n-hexane, ethyl acetate, n-butanol, and water. Two compounds were isolated by using an ODS column chromatography from ethyl acetate soluble fraction shown to the strongest antioxidant activity ($RC_{50}=3.15\;{\mu}g/m{\ell}$) among the fractions. The isolated compounds were analyzed by $^1H$ and $^{13}C$ NMR, IR, UV/VIS, MS spectrum data and identified as catechin, ${\rho}-Hydroxyphenethyl$ alcohol $1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$. The compounds have shown strong antioxidant activity, with similar activity to BHA ($RC_{50}=2\;{\mu}g/m{\ell}$). Especially, ${\rho}-Hydroxyphenethyl$ alcohol 1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$ was shown strong anti-lipid peroxidative activity. However, the compounds were not shown antimicrobial activities. In antimicrobial activity assays, ethyl acetate soluble fraction was effective to bacterial inhibition, such as Escherichia coli and Klebsiella pneumonia, with minimum inhibitory concentrations in $125\;{\mu}g/m{\ell}$. Otherwise, antifungal activity against Candida albicans was shown in n-hexane soluble fraction exhibiting $63\;{\mu}g/m{\ell}$ of minimum inhibitory concentration. In anticomplementary activity assays, water soluble fraction was the most effective exhibiting 24% inhibitory activity.

Experimental Studies on Lead Toxicity in Domestic Cats 1. Symptomatology and Diagnostic Laboratory Parameters (고양이의 납중독에 관한 실험적 연구 1. 임상증상 및 실험실적 평가)

  • Hong Soon-Ho;Han Hong-Ryul
    • Journal of Veterinary Clinics
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    • v.10 no.1
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    • pp.111-130
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    • 1993
  • Lead toxicity was evaluated in forty-five cats on a balanced diet, Treated with 0(control), 10, 100(low), 1, 000, 2, 000, and 4, 000(high) ppm of lead acetate orally on a body weight basis. The objectives were to establish toxic dosage level of leaf in cats, to characterize changes in behavior and clinical pathology, and to demonstrate what blood lead concentrations correlate with the known dosages of lead. Some high dose cats showed projectile vomiting, hyperactivity, and seizures. The growth rates did not appear to be altered in any of the dosed groups. Normal blood lead concentration in cats were lower than that of humans, dogs, and cattle. Blood lead concentrations of 3 to 20$\mu\textrm{g}$/100$m\ell$ could be termed a 'subclinical' range in the cat. Clinical lead toxicity in cats may have blood lead concentrations ranging 20 to 120$\mu\textrm{g}$/100$m\ell$. Zinc protoporphyrin concentrations were proportional to lead dosages and a significant ZPP elevation, greater than 50$\mu\textrm{g}$/100$m\ell$, may be indicative of clinical lead toxicity. The enzyme aminolevulinic acid dehydratase showed an inverss dose response relationship for all lead dosages and a significant ZPP elevation, greater than 50$\mu\textrm{g}$/100$m\ell$, may be indicative of clinical lead toxicity. The enzyme aminolevulinic acid dehydratase showed an inverse dose response relationship for all lead dosages and appears to be a good indicator of lead exposure in cats. Urinary aminolevuliruc acid concentrations generally increased with lead dosage, but individual values varied. Hair lead concentrations rose proportionately to lead dosages. Lead at least in high doses appears to inhibit chemotactic activity of polymorphonuclear cells and monocytes. No consistent dose response relationships were observed in hemoglobin, RBC, WBC, neutrophil, lymphocyte, monocyte, and eosinophil counts. There were no consistent dose related changes in total protein, plasma protein, BUN, and ALT values. Reticulocyte counts did not increase significantly in most lead dosage levels, and are probably of little value in diagnosing lead toxicity in cats. The fact that no significant changes were found in nerve conduction velocities may support that there was no segmental demyelination resulting from lead ingestion. The lethal dose in cats appear to range from 60 to 150mg/kg body weight. A reliable diagnosis of lead poisoning can be made utilizing blood lead, ZPP, and ALAD, and hair lead.

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Quality Characteristics and Antioxidant Activity of Commercial Doenjang and Traditional Doenjang in Korea (국내 시판된장 및 재래된장의 품질 및 항산화 특성)

  • Ahn, Jun-Bae;Park, Jin-A;Jo, Hye-Ji;Woo, In-Hye;Lee, Sang-Hwa;Jang, Keum-Il
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.142-148
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    • 2012
  • This study compared the quality and antioxidant characteristics of commercial and traditional Doenjangs from various regions of Korea. The pH, salinity, and brix degree of the samples tested ranged from 4.61~6.36, 0.90~1.00% and 0.97~1.10 $^{\circ}Bx$, respectively. The pH was highest for Doenjang made in the Jeonnam region. No significant differences in the salinity or brix degree of Doenjang from different regions of Korea were found(p>0.05). The L, a, and b-values for color of the Doenjang samples ranged from 52.92~55.00, 0.03~0.62 and -0.74~2.70, respectively. The total polyphenol content(TPC) ranged from 18.71~25.78 mg GAE/$m{\ell}$, and the 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2-azinobis(3-ethyl benzothiazoline)-6-sulfonic acid(ABTS) radical scavenging activities ranged from 6.43~14.38 and 2.46~7.08 mg AE/$m{\ell}$, respectively. The TPC was highest for Doenjang from Gyeongnam, while that from Gyeongbuk had the lowest content. The DPPH radical scavenging activity was highest for Doenjang from Jeonnam and lowest for Doenjang from Gangwon. The ABTS radical scavenging activities for Doenjang from Gyeonggi and Gyeongbuk were lower in comparison to samples from other regions. Consequently these results should provide better information for standardizing and improving the quality and functional activity of commercial Doenjang in Korea.

In vitro Fermentation Characteristics of Juice Pomaces Using Equine Fecal Inoculum (말 분변을 이용한 주스박의 in vitro 발효 특성)

  • Hwang, Won-Uk;Kim, Gyeom-Heon;Lim, Joung-Ho;Woo, Jae-Hoon;Park, Nam-Geon;Kim, Soo-Ki
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.4
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    • pp.322-331
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    • 2017
  • This study was conducted to evaluate the changes of pH, dry matter digestibility (DMD), $NH_3-N$ concentrate, gas production and volatile fatty acid (VFA) through in vitro fermentation by adding horse feces to various juice pomaces fermented with Bacillus, yeast and lactic acid bacteria. The pH range of fermented fluid with juice pomaces was 6.4-7.1, indicating that the digestion by microbial fermentation was normal. Juice pomaces adopted will be helpfully used to assist with digestion by microbes in intestines because approximately $10^9CFU/m{\ell}$ microbes were grown after 48 hours in fermented fluid. DMD rate gradually increased from 12 hours. It was 39.19% in pomaces of apple, 38.22% in grape, 37.02% in carrot, 36.2% in citrus and 34.35% in mixture respectively after 48 hours. $NH_3-N$ concentrate was not changed significantly as it was maintained at $1.5mg/100m{\ell}$ level in the entire treatment group from beginning of fermentation until 12 hours, but increased rapidly from 24 hours. Amount of gas produced was lowest in the mixture and increased rapidly after 12 hours. Total VFA increased from 24 hours and was highest at 48 hours. It was suggested that dry matter digestion was processed while fermented juice pomaces kept proper pH during in vitro digestion, and cellulose degrading microorganisms could act actively in the caecum and colon of horses.