• Korean Journal of Microbiology
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• v.35 no.2
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• pp.128-132
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• 1999

The effects of diet on the composition of fecal microflora in mouse and the aclivilies of several enzymes in the leces were investigated. Vegetarian dietary groups were found to contain about ten lines higher numbers of Locmbocillus and B$&bacterizml than animal dietary groups. An~rnal dietmy groups were found to contain about 5 tolo times higher numbers of anaerobic Closhidia and Bocieriocles than the vegeterian detary groups. Fccal microbial $\beta$-glucosidase, $\beta$-glucm'onidase, ii-yptophanase and orease activilies in ihe animal dietary groups were shown lo be 30 to 50% hgher than those in h e vegetarian detary groups.

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.37-43
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• 1998

The highest transformation frequency was observed when cotyledonary and hypocotyl explants of flowering cabbage (Brassica oleracea var. acephala DC) 'Eunbae' were cultured on shoot induction medium without kanamycin for 1 day, then cocultured with Agrobacterium tumefaciens LBA4404;;pGA1036 harboring tomato inhibitor II promoter and $\beta$-glucuronidae (GUS) fusion gene for 3 days. These explants were transferred to MS medium containing 20 mg/L kanamycin, 500 mg/L carbenicillin, and 1 mg/L BA. The explants were subsequently subcultured every 2 weeks. Incorporation of the GUS gene into flowering cabbage was confirmed by PCR analysis of DNA. Southern blot analysis showed that ECL-labeled GUS gene was hybridized to the expected amplified genomic DNA fragment of about 366 bp from transgenic flowering cabbage. Histochemical analysis based on the enzymatic activity of the GUS protein indicated that PI-II promoter activity was sysmatically associated with vascular tissue in wonded as well as in non-wounded leaves, petioles and stems, but not in roots. Partial wounding with razor blade showed not systemic induction but partial induction.