• 제목/요약/키워드: $\beta$-carotene lipid

검색결과 129건 처리시간 0.026초

한국인 제2형 합병증동반 당뇨병 환자에 있어 과산화지질, 항산화 효소, 및 항산화비타민에 관한 연구 (The Study of Lipid-peroxidation, Antioxidant Enzymes, and the Antioxidant Vitamins in NIDDM Patients with Microvascular-diabetic Complications)

  • 하애화
    • Journal of Nutrition and Health
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    • 제32권1호
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    • pp.17-23
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    • 1999
  • The purpose of this study was to determine the extent of oxidative stress in NIDDM patients with diabetic complications and to determine the relationship between oxidative stress and diabetic complications. For this study, 139 NIDDM patients were recruited, 85 with diabetic complications and 54 without complications were recruited. The concentration of malondialdehyde(MDA) and the activities of antioxidant enzymes including catalase, superoxide dismutase(SOD), gluthatione peroxidase(GSH-Px)were determined. The daily intakes and plasma concentrations of beta-carotene, lycopene, lutein nd alpha-tocopherol were determined by food frequency questionnaire and by high performance liquid chromatography(HPLC), respectively. Among the antioxidant enzymes studied, only GSH-Px activity was lower in NIDDM patient, with diabetic complications than in those without complications(2.91$\pm$0.80 vs 3.54$\pm$0.44 U/mgHb, p<0.05). Those NIDDM patients with diabetic complications had higher MDA concentrations than those without diabetic complications(1.40$\pm$0.25 vs 1.25$\pm$0.11 nmol/ml, p<0.05). There were no significant differences in the dietary intakes of total carotenoids(2854 vs 2824ug/day)or vitamin E (9.5$\pm$3.2 vs 9.5$\pm$2.0mg/day)between NIDDA patients with and without complications. However, the plasma concentrations of beta-carotene and lycopene were significantly lower in NIDDM patients with complications than in NIDDM patients without complications (Beta-carotene : 24.2$\pm$12.5 vs 33.1$\pm$16.2(ug/dl), lycopene : 2.8$\pm$2.1 vs 4.3$\pm$2.8(ug/dl)). This study showed that in NIDDM patients with complications, the lipid peroxidation of erythrocytes was higher increased and the antioxidant reserves were significantly dipleted, compared with NIDDM patients without complications. The lower plasma concentrations of beta-carotene and lycopene in NIDDM patients may be due to the presence of diabetic complication, not due to the lower dietary intakes of antioxidant vitamins. To define the role of carotenoids in diabetes, more experimental and clinical studies are needed.

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모델시스템에 있어서 무지개 송어 지방질의 산화에 대한 Lipoxygenase의 영향 (Effect of Lipoxygenase on the Oxidation of Rainbow Trout Lipid in Model system)

  • 김혜경;엄수현;최홍식
    • 생명과학회지
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    • 제5권2호
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    • pp.70-75
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    • 1995
  • The effect of lipoxygenase (LOX) on the oxidation and co-oxidation of lipid fraction was studied in the model system of rainbow trout. For the reaction in model system 1 g of lipid fraction and 50mL of enzyme extract(LOX, 140 unit in 50mL phosphate buffer solution at pH 7, 4)), which were obtained from rainbow trout, were homoginized in the presence of Tween 20 and kept at 23$\circ$C for 3 days. The activity of LOX was decreased to 43% of initial level during the reaction in the model system. The initial composition of rainbow trout lipid was showed to be consisted of trigliceride(TG;82%) and free fatty acid(FFA;0.1%), while this converted to 59% of TG and 20% of FIFA, respectively after reaction in model system. Change of fatty acid composition was also observed and the content of linoleic acid, one of the major fatte acids, was decreased to 13% from 54% in the content of total fatty acids after reaction. The carotenoids in rainbow trout were composed of 0.4% $\alpha$-carotene, 1.6% $\beta$ -carotene, 80% canthaxanthin, 7% lutein and 11% zeaxanthin, thus the canthaxanthin was the major component. This canthaxanthin was the most degraded carotenoid by lipoxygenase catalyzed co-oxidation during the reaction. On the other hand the tocopherol isomers found in the rainbow trout were $\alpha$ and $\beta$ -tocopherol, and $\alpha$-tocopherol had a higher degradation rate by the lipoxygenase catalyzed co-oxidation than of $\beta$-tocopherol in the reaction of model system.

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Evaluation of Antioxidative activity of Korean Yam (Dioscorea batatas DECNE.) by n-Butanol and Ethyl Acetate Extracts

  • Duan, Yishan;Kim, Han-Soo;Kim, Gyeong-Hwuii
    • 한국응용과학기술학회지
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    • 제32권2호
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    • pp.312-319
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    • 2015
  • In this study, n-butanol and ethyl acetate extracts were prepared from raw yam (Dioscorea batatas DECNE.). Their antioxidative potencies were investigated employing various in vitro methods, such as ferrous ion chelating, ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and nitric oxide (NO) radical scavenging and nitrite scavenging activity. The n-butanol fraction was assayed to possess stronger antioxidant activity by ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and NO radical scavenging activity. However, ethyl acetate extract was more effective in chelating ferrous ion and scavenging nitrite. Based on the results obtained, yam is a potential active ingredient that could be applied in antioxidation as well as bio-health functional food to take a good part in prevention of human diseases and aging.

Antioxidant effect of myricetin with other antioxidants, taurine and $\beta$-carotene on mouse melanoma cell

  • Yu, Ji-Sun;Kim, An-Keun
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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    • pp.69-69
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    • 2003
  • There are now increasing evidences that free radicals and reactive oxygen species are involved in a variety of pathological events. Reactive Oxygen Species (ROS) are produced during normal cellular function. ROS lead to lipid peroxidation, massive protein oxdiation and degradation. Under normal conditions, antioxidant are substnaces that either directly or indirectly protect cell against adverse effect of ROS. several biologically important compound include ${\beta}$-carotene, taruine and flavonoids reported have antioxidant function. The various antioxidant either scavange superoxide and free radicals or stimulate the detoxification mechanisms within cells resulting in increased detoxification of free radicals formation and thus in prevention of many pathophysiologic processes. This study carried out to investigate the antioxidant activity of flavonoids, myricetin with other antioxidants, ${\beta}$-carotene and taurine on B16Fl0. In order to investigate the efficacy of antioxidant activity, we measured cell viability, antioxidant enzyme activity (SOD, GPX, CAT) and intracellular reactive oxygen intermediate (ROI). In this results, we show that these flavonoids with other antioxidant substrates are increased antioxidant activity level.

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튀김용 돈지의 열산화 안정성에 미치는 $\beta-Carotene$의 효과 (Effects of $\beta-Carotene$ on the Thermal Oxidation Stability of Deep Fried Lard)

  • 한규호;박표잠;전병태;박우준;이치호
    • 한국축산식품학회지
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    • 제25권4호
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    • pp.507-512
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    • 2005
  • 본 연구는 $80^{\circ},\;130^{\circ}C$$180^{\circ}C$온도대별로 유열 처리한 돈지에 $0\%,\;0.001\%$$0.004\%$의 수준으로 $\beta-carotene$을 첨가한 후 $50^{|circ}C$에서 15일간 보관시키면서 $\beta-carotene$ 총의 열 산화 안정성을 알아내기 위하여 총 균수검사, 과산화물가 (Peroxide value), 산가(Acid value) 및 TBA가를 측정하여 비교한 결과 다음과 같은 결과를 얻었다. 1) 미생물 총 균수 실험에서 $\beta-carotene$의 첨가량이 $0\%,\;0.001\%$$0.004\%$로 증가함에 따라 균의 증식이 현저하게 억제되었다. 온도대별로 $180^{\circ}C$ 처리 군에서는 저장기간이 길어질수록 총 균수가 증가하는 경향을 나타내었으나, $80^{\circ}C$$130^{\circ}C$ 처리 군에서는 저장 기간이 길어질수록 총 균수가 현저하게 증가하였다. 2) 과산화물가의 측정 실험에 $\beta-carotene$의 첨가량이 $0.004\%$일 때가 $0\%,\;0.001\%$에 비해 유의하게 (P<0.05) 낮은 값을 나타냈다. 3) 산가의 측정실험에서 저장기간이 길어지면서 $\beta-carotene$의 첨가량이 증가할수록 항산화 능력과는 정의 상관관계를 나타났다. $\beta-Carotene$의 첨가량이 $0.004\%$일 때가 $0\%,\;0.001\%$에 비해 유의하게 (P<0.05) 낮은 값을 나타냈다. 4) TBA가 측정실험에서 대조군과 시료와의 비교에서 온도가 증가함에 따라 값이 상승하는 경향을 나타냈으며, 저장 기간 15일째 $\beta-carotene$의 첨가량이 $0.001\%$$0.004\%$ 처리 군이 대조군에 비해 유의하게 (P<0.05) 낮은 값을 나타냈다. 따라서 유열처리 한 돈지에 $0.004\%$ $\beta-carotene$을 첨가하면 열 산화 방지에 매우 효과적인 것으로 사료되었다.

Effects of Multivitamin-Mineral Supplementation, at Nutritional Doses, on Plasma Antioxidant Status, Erythrocyte Antioxidant Enzyme Activities and Lipid Peroxidation during Pregnancy

  • Park, Eunju;Kang, Myung-Hee
    • Nutritional Sciences
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    • 제3권1호
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    • pp.36-41
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    • 2000
  • The aim of this study was to evalute the effect of multivitamin-mineral supplementation during pregnancy on plasma levels of antioxidants, erythrocyte antioxidant enzyme activities, and lipid peroxidation. A controlled, semi-randomized, prospective trial was performed by comparing the supplement group, which received multivitamin-mineral tables once daily for 10 weeks, with the control group. Plasma levels of $\beta$-carotene, tocopherol, coenzyme Q10, ascorbate, folate, zinc, and selenium and malondialdehyde (MDA), as well as the activities of superocxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in erythrocytes were measured initially (20 wk gestation) and at the end of the intervention (34 wk gestation). In the control group, plasma ascorbate and selenium levels decreased and tocopherol levels increased. In the supplement group, a significant increase in plasma $\beta$-carotene(46%), conenzyme Q10 (42%), and zinc (24%) was observed after 10 weeks of supplementation. No changes were observed in the plasma levels of MDA, and erythrocyte GSH-Px activity, while SOD activity increased in both control group and the supplement group during the intervention. These data suggest that multivitamin-mineral supplementation during pregnancy produced moderate increases in plasma $\beta$-carotens, coenzyme Q10, and zinc concentrations but the enhancement of those plasma antioxidants had on direct on the plasma level of MDA, erythrocytes SOD or GSH-Px activities.

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Effects of the P/S Ratio of Dietary Lipids and Antioxidant Vitamin Supplements on the Level of Serum Lipids and Liver. Lipid Peroxidation in Rats Treated with DMBA

  • Lee, Byung-Joo;Park, Jung-Nan;Lee, Sang-Sun
    • Journal of Nutrition and Health
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    • 제31권5호
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    • pp.906-913
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    • 1998
  • This study an analyzes the effects of the P/S ratio of dietary lipids and antioxidant vitamin supplements on serum lipids level and fatty acid profile, the degree of lipid peroxidation, and the antioxidant enzyme activities in the liver of rats treated with 7,12-dimethylbenz($\alpha$) anthracene(DMBA). P/S ratio of dietary lipids was made into 0.5, 1 and 2 by mixing palm oil, soybean oil, sesame oil and perilla oil at 10%(w/w) fat level and n-6/n-3 ratio was fixed to 4. Antioxidant vitamin of $\alpha$-tocopherol or $\beta$-carotene was supplemented in addition to vitamin mixture which was given at 1 % of the standard diet. female Sprague-Dawley strain rats, about 60 days old, were divided into three groups(LP : low P/S ratio(0.5), MP : medium P/S ratio (1.0), HP , high P/S ratio(2.0)) and each group was sub-divided into three groups(S ; standard, T ; tocopherol supplemented, C : carotene supplemented): Two weeks after feeding experimental diets, all groups were treated with a single dose of DMBA(2mg/100g BW) by gastric intubation and fed experimental diet for 9 week. The results were as follows ; 1) Serum total cholesterol(TC) level was not significantly influenced by diet but tended to be lower in HP groups compared to LP and MP groups. Triglyceride level was the highest in LP groups and the lowest in $\alpha$-tocopherol supplemented groups. 2) Thiobarbituric acid reactive substance(TBARS) level, representing lipid peroxidation in hepatic microsome, tended to be increased as the unsaturation of dietary lipids increases. $\alpha$-Tocopherol supplement significantly decreased TBARS level. 3) The activities of superoxide dismutase(SOD) and glutathione peroxidase(GSHPx) in hepatic cytosol showed the tendency to be high with increasing P/S ratio of dietary lipids. SOD activity was not significantly influenced by antioxidant vitamin, but GSHPx activity was significantly increased in $\alpha$-tocopherol supplemented groups. In summary, high polyunsaturated fat diet was effective on reducing the serum level of total cholesterol and triglyceride, while it increased unsaturation and peroxidizability of serum fatty acid. With increasing P/S ratio of dietary lipids, lipid peroxidation was increased in the liver and antioxidant enzyme system was induced to inhibit lipid peroxidation against oxidative damage. $\alpha$-Tocopherol supplement was effective in lowering lipid peoxidation, but $\beta$-carotene supplement did not exhibit antioxidant effect. (Korean J Nutrition 31(5) 906~913, 1998)

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일부 남녀 대학생에서 혈장 호모시스테인 농도와 산화 스트레스 지표와의 상관관계 (Relationships of Plasma Homocysteine Concentration and Oxidative Stress Markers in Korean Collage Students)

  • 김정신;박은주;민혜선;강명희
    • Journal of Nutrition and Health
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    • 제43권5호
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    • pp.443-452
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    • 2010
  • Elevated plasma concentration of total homocysteine (ptHcy) is known as an independent risk factor of cardiovascular disease (CVD) and oxidative stress is also commonly implicated in CVD. An association between ptHcy and oxidative stress has recently been suggested. The study objective is to examine the relationship between ptHcy and oxidative stress markers in 103 healthy college students (62 males and 41 females). Plasma levels of ptHcy, oxidative stress markers (conjugated diene, erythrocyte catalase, TRAP, lymphocyte DNA damage), antioxidant vitamins ($\alpha$-tocopherol, $\gamma$-tocopherol, carotenoids), and lipid parameters (total cholesterol, triglyceride, HDL cholesterol) were determined. The results show that the concentration of ptHcy was significantly higher in male subjects ($22.17\;{\pm}\;2.14\;{\mu}mole/L$) than in female subjects ($12.28\;{\pm}\;0.45\;{\mu}mole/L$). There was a negative association between ptHcy and plasma ${\beta}$-carotene in male subjects (p $lt; 0.05), but no correlation between ptHcy and other plasma antioxidant vitamin levels in either gender. However, there were the negative correlations between ptHcy and plasma ${\alpha}$-carotene or ${\beta}$-carotene, and a positive correlation between ptHcy and lymphocyte DNA damage. A significantly low level of ${\alpha}$-carotene or ${\beta}$-carotene was found in male subjects with elevated ptHcy (${\geq}\;15\;{\mu}mol/L$), as compared to those with lower plasma homocysteine. These study results confirmed the views on the association between plasma homocysteine and oxidative stress markers in humans and support the hypothesis that homocysteine promotes the oxidative environment by counteracting the antioxidant defense mechanism.

미생물(微生物)에 의(依)한 Carotenoid색소(色素) 생성(生成)에 관(關)한 연구(硏究) [제1보(第1報)] - Rhodotorula glutinis var. glutinis sw-17의 Carotenid생성(生成)에 관(關)하여 - (Studies on the Production of Carotenoid Pigments by Microorganism [Part I] - The Carotenoid Production in Rhodotorula glutinis var. glutinis Sw-17 -)

  • 박기현;박성오
    • Applied Biological Chemistry
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    • 제19권4호
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    • pp.243-247
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    • 1976
  • 토양(土壤)으로부터 분리동정(分離同定)된 우수(優秀)한 세포내(細胞內) 지질생성균주(脂質生成菌株)인 Rhodotorula glutinis var. glutinis sw-17에 대(對)하여 carotenoid생성능(生成能)을 검토(檢討)하고서 이 균주(菌株)를 $25^{\circ}C$에서 8일간(日間) 진환배양(振還培養)하여 caroteneid 색소(色素)를 추출분리(抽出分離)하여 column에 흡착(吸着)된 색소층(色素層)의 순서(順序)와 용출(溶出)하여 정제(精製)한 각색소(各色素)의 흡수곡선(吸收曲線)에 의하여 각각(各各)의 carotenoid를 확인(確認)했으며 이들의 흡광도(吸光度)를 측정(測定)하여 Beer의 법칙(法則)에 따라 참고문헌(參考文獻)의 흡광계수(吸光係數)를 사용(使用)하여 정량(定量)한 결과(結果) torularhodin 25.52%, torulene 38.16%, neuroporene 1.49%, ${\gamma}-carotene$ 9.88%, ${\beta}-zeacarotene$ 2.0%, ${\beta}-carotene$ 19.95%이었으며 ${\delta}-carotene$은 거의 흔적(痕蹟)에 가까웠다. 따라서 Rhodotonula glutinis var. glutinis sw-17의 주색소(主色素)는 torularhodin, torulene이었다.

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고압 액체 크로마토그라피에 의한 알파, 베타, 카로텐, 루테인 및 리코펜의 항산화효과 비교 연구 (Comparison of Antioxidant Activity of $\alpha$-, $\beta$-Carotene, Lutein and Lycopene by High Pressure Liquid Chromatography)

  • Kim(Jun), Hyeyoung
    • Journal of Nutrition and Health
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    • 제23권6호
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    • pp.434-442
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    • 1990
  • 과산화지질 반응 계에서 말론알데히드 정량을 위한 HPLC방법을 개발하였으며, 알파-,베타- 카로텐, 루테인 및 리코펜의 간 마이크로좀 시스템에서의 항산화작용을 새로 개발한 HPLC 방법과 TBA 값으로 비교하였다. 해동횟수에 따른 간 마이크로좀의 말론알데히드 형성능력을 비교하였다. 결과로서, 말론알데히드는 5$\cdot6분만에 확인되었으며, pack area와 농도는 직선 관계를 나타내었다. 해동횟수 증가에 따라 마이크로좀의 지질과 산화 능력은 감소되었으며, 이는 세포막의 파괴에 기인한것으로 사료된다. Fe$^{+3}$-ADP/NADPH와 paraquar/NADPH 반응계에서 리코펜, 루테인 및 알파-카로텐은 베타-카로텐이나 토코페롤보다 강력한 항산화효과를 나타내었다. Fe$^{+3}$-ADP/NADPH 반응 계에서 카로테노이드와 토코페롤의 과산화지질 형성 억제효과는 TBA 값과 HPLC에 의한 말론알데히드 정량에 의해 비교해볼때 유사한 효과를 나타내었다.

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