• YAKHAK HOEJI
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• v.49 no.4
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• pp.312-316
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• 2005

To develop a new natural antibiotics from Koran plant resources for dealing with the current situation regarding the rapid increase in antibiotic-resistant pathogen, the in vitro inhibitory activities of essential oils from the young leaves of Glehnia littoralis (Umbelliferae) as well as its main constituents were evaluated against susceptible and resistant species of Streptococcus pneumoniae. The essential oil fraction of G. littoralis and its main components, $\alpha-and\;\beta-pinene$, exhibited significant inhibitory activities against the antibiotic-susceptible and resistant strains of S. pneumoniae, with MICs (minimum inhibiting concentrations) ranging from 4.0mg/ml to 16mg/ml. No remarkable differences were shown between the susceptible and resistant strains. Moreover, the disk diffusion test disclosed that these inhibitory activities were dose­dependent. Furthermore, data from the checkerboard titer test with FICIs (fractional inhibiting concentration indices) from 0.15 to 0.28 indicated synergisms between norfloxacin and $\alpha-or\;{\beta}-pinene$ in activity against S. pneumoniae KCCM49629 and S. pneumoniae CCARM4059.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.1
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• pp.66-78
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• 1992

The essential oil of Korean valerian root ( Valeriana fauriei roar. dasycarpa Hara) was isolated by simultaneous distillation Sl extraction. The oil content of fresh root was 0.7% (wb) and that of dried root was 2.1 5 (db) and sensory analysis of the oil indicated sweet-balsamic, woody and floral characteristic aroma notes. The oil was fractionated into one hydrocarbon fraction and three oxygenated hydrocarbon fractions by using silica gel column chromatograpy. Each fraction was analyzed by capillary GC and GC-MS. Out of 81 characterized compounds, the major compounds were a-pinene, camphene, $\beta$-pinene, bornyl acetate, borneol , bornyl iso-valerate and sesquiphellandrene and the characteristic floral and woody aroma of neutral fraction of Korean valerian root could be due to be the presence of oxygenated compounds such as borneol, bornyl acetate, bornyl iso-valerate, p-ionone ana $\beta$-ionone epoxide. Comparing the yield of Korean valerian root with those from other origins reported, oil content of Korean valerian root was higher than those of European and Indian origins.

• KSBB Journal
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• v.17 no.4
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• pp.357-364
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• 2002

For the effecient extraction methods of essential oil, pretreatment of leaves, ratios of water and leaves, extraction time, and collection season from the Chamaecyparis obtusa and Chamaecyparis pisifera leaves were studied. The chemical composition of essential oil was analyzed by GC-MS. The yield of essential oil from ground leaves was higher than that of chopped leaves. The yield of essential oil was not affected much by mixing ratios of water and leaves. The yield of essential oil reached maximum after 5 hours. The content of essential oil of C. obtusa leaves collected during winter was 4.5%, whereas the content of essential oil of C. pisifera collected during fall was 5.3%. The composition of essential oils extracted form C. obrusa and C. pisifera was different. The major constituents in the essential oil of C. obtusa were monoterpene as limonene, terpinene-4-ol, ${\gamma}$-selinene, and a-cedrene, and those of C. pisifera was monoterpens as ${\alpha}$-pinene, myrcene, limonene, bornyl acetate, ${\beta}$- caryophyllene, longifolene, and ${\beta}$-cedrene.

• Korean Journal of Food Science and Technology
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• v.24 no.5
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• pp.423-427
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• 1992

The volatile components were extracted from kumquat(Fortunella margarita) by simultaneous steam distillation-extraction method and fractionated on silica gel column. The total volatile oil was eluted off first by n-pentane and eluted again by diethyl ether. The total volatile oil and diethyl ether fraction were analyzed by GC and GC-MS. In the total volatile oil, 10 components were identified, of which major ones were limonene(96.5%, of total volatile oil), ${\beta}-pinene$(1.93%) and ${\alpha}-terpineol$(0.42%) and then the characteristic aroma of kumquat appeared to be due to limonene. On the other hand diethyl ether fraction, from which 46 components were identified, contained 9 alcohols, 22 terpenes and terpene alcohols, 7 aldehydes and ketones, 7 esters and 1 miscellaneous components. The major components were ${\alpha}-terpineol$(31.98% of diethyl ether fraction), ${\beta}-terpineol$(7.37%), geranyl acetate(9.69%) and p-menthadien-9-ol(4.12%).

• Korean Journal of Pharmacognosy
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• v.24 no.2
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• pp.111-115
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• 1993

Essential oil of the root of Angelica koreana Max. (Umbelliferae) was investigated. Essential oil was obtained from the dried roots by steam distillation and fractionated by column chromatography. Each isolate or fraction was identified by GC, GC-MS and spectral analysis. It was found to contain nine monoterpenes such as ${\alpha}-pinene$(7.0%), camphene, ${\beta}-pinene$, myrcene, ${\alpha}-phellandrene$, ${\Delta}-3-carene$, p-cymene, limonene(2.8%), terpinolene and also found to contain m-cresol(11.6%), citronellol, citral b, methylcinnamate, eudesmol and osthol. 2-Hydroxy-5-methylacetophenone and twelve compounds were tentatively identified.

• Korean Journal of Pharmacognosy
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• v.7 no.4
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• pp.237-240
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• 1976

To investigate the constituents of following plants, Valeriana fauriei species, which are V. fauriei var. fauriei $H_{ARA}$, V. fauriei form. coreana $H_{ARA}$ and V. fauriei var. dasycarpa $H_{ARA}$, we carried out gas-liquid chromatographical analysis of the volatile oils of these plants and found that camphene, l-bornylacetate, ${\alpha}-pinene,\;{\beta}-pinene$, d,l-limonene, l-borneol and ${\alpha}-kessylacetate$ were common components in their volatile oils. However, their terpenoidal composition was considerably different from ${\alpha}-kessylacetate$ in V. faurie var. fauriei, from kessanol, ledol, kessoglycol and nardol in V. fauriei var. dasycarpa, and from kessoglycoldiacetate, ${\alpha}-kessylalcohol$, faurinone and terpineol in V. faurie form. coreana. It was found by gas-liquid chromatography that the terpenoidal patterns of these Korean valerians were similar to those of Japanish valerians.

• Natural Product Sciences
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• v.10 no.6
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• pp.325-329
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• 2004

The composition of essential oil from O. javanica was analyzed by gas chromatography-mass spectrometry. Using the broth dilution method and disk diffusion test, anti-microbial activities of the oil fraction and its main components were evaluated against various antibiotic-susceptible and resistant strains of pathogenic microorganisms. As a result of GC-MS analysis, 57 compounds, including ${\alpha}-terpinolene$ (28.1%), dl-limonene (16.0%), ${\gamma}-terpinene$ (10.3%), ${\beta}-pinene$ (9.7%) and ${\alpha}-pinene$ (6.0%) were identified in the essential oil fraction. The essential oil fraction of O. javanica and its main components exhibited significant inhibitory activities, particularly against Candida albicans (antibiotic-susceptible strains) and Streptococcus pneumoniae (antibiotic- susceptible and resistant strains). The main components of the O. javanica oil fraction displayed different patterns of activity against the three tested Candida species as exemplified by the differential minimum inhibiting concentration (MIC) values. The disk diffusion test showed that the activities were dose dependent.

• Journal of Applied Biological Chemistry
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• v.58 no.2
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• pp.105-108
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• 2015

This research is aimed at evaluating the potential abilities of the natural acaricide of F. suspense oil against Tyrophagus putrescentiae and Dermatophagoides spp. Based on the $LD_{50}$ values, in contact bioassay, F. suspense oil (8.19, 3.28, and $4.35{\mu}g/cm^2$) showed acaricidal effects against T. putrescentiae, D. farinae, and D. pteronyssinus, respectively. Fumigant toxicities of F. suspense oil showed similar patterns as those observed with contact toxicities. GC/MS analysis showed the major components of F. suspense oil to be ${\beta}$-pinene (45.88%), myrtenol (13.86%), (+)-${\alpha}$-pinene (13.09%), (-)-trans-pinocarveol (7.34%), sabinene (6.64%) and pinocarvone (4.13%). These findings indicate that F. suspense oil has potential as a natural acaricide.

• Applied Biological Chemistry
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• v.37 no.1
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• pp.1-8
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• 1994

This study was carried out to investigate the changes in volatile constituents concerning with the flavor of the green ginger (Zingiber officinale Roscoe) during storage in underground pit ($15^{\circ}C$, RH 95%). And the constituents of essential oil of etiolated shoots formed on the mother rhizomes during the five months storage in the dark under same conditions were compared with those of mother rhizomes. The essential oils of Korean domestic ginger (Bong-dong cultivar) were isolated by simultaneous steam distillation and extraction method (SDE). Then the compositions of the essential oils were analysed by GC and GC-MS spectrometry. The major compounds of essential oil from the fresh rhizomes were zingiberene, $citronellol+{\beta}-sesquiphellandrene$, ${\beta}-phellandrene$, camphene, geranial, ${\gamma}-bisabolene$, ar-curcumene+geranyl acetate, ${\alpha}-pinene$, ${\beta}-gurjunene$, limonene and neral. The content of monoterpene hydrocarbons increased with a concomitant lowering in the amounts of sesquiterpene hrdrocarbons and oxygenated sesquiterpenes during storage of rhizomes although contents of the oxygenated monoterpens changed little or slightly during the storage. During the storage the content of such monoterpenes as camphene, ${\beta}-phellandrene$ and citral (neral and geranial) increased whereas the content of such sesquiterpenes as zingiberene and $citronellol+{\beta}-sesquiphellandrene$ decreased. The composition of shoot oil differed from that of mother rhizome oil in having higher content of terpene hydrocarbons and also in the higher content of bornyl acetate, ${\beta}-gurjunene$ and ar-curcumene+geranyl acetate and lower in citral (neral and geranial).

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.30-34
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• 2016

To evaluate the efficacy of natural nematicides for the control of root-knot nematode in strawberry greenhouses, commercial essential oils were examined by 24-well culture plate bioassay for their nematicidal activities against second-stage juveniles and eggs of Meloidogyne hapla. Based on the mortality of M. hapla juveniles at a concentration of $125{\mu}g/mL$, the most active essential oil was Alpinia galanga (100%), followed by Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%), and Foeniculum vulgare (2.1%). Moreover, A. galanga significantly reduced hatching at 7, 14, and 21 days after treatment. The volatile constituents identified in the A. galangal oil were methyl cinnamate (87.4%), 1,8-cineole (4.4%), ${\beta}$-pinene (2.5%), ${\alpha}$-pinene (2.2%), and p-cymene (1.1%), as major constituents. Results of this study show that A. galangal essential oil and its major constituents may serve as an environmental friendly agent of a promising natural nematicide to control Meloidogyne spp.