• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.256-262
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• 2011

A 20.9-kDa metalloprotease was isolated from dried fruiting bodies of the wild basidiomycete mushroom Lepista nuda. The N-terminal amino acid sequence of the protease was seen to be ATFVLTAATNTLFTA, thus displaying no similarity with the sequences of previously reported metalloproteases. The protease was purified using a procedure that entailed ion-exchange chromatography on CM-Cellulose, Q-Sepharose, and Mono S, and FPLC-gel filtration on Superdex 75. The protease functioned at an optimum pH of 7.0 and an optimum temperature of $50^{\circ}C$. It was also noted that the protease demonstrated a proteolytic activity of 1,756 U/mg toward casein. The $K_m$ of the purified protease toward casein was 6.36 mg/ml at a pH of 7.0 and with a temperature of $37^{\circ}C$, whereas the $V_{max}$ was 9.11 ${\mu}g\;ml^{-1}\;min^{-1}$. The activity of the protease was adversely affected by EDTA-2Na, suggesting that it is a metalloprotease. PMSF, EGTA, aprotinin, and leupeptin exerted no striking inhibitory effect. The activity of the protease was enhanced by $Fe^{2+}$, but was curtailed by $Cd^{2+}$, $Cu^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ ions. The protease also exhibited inhibitory activity against HIV-1 reverse transcriptase with an $IC_{50}$ value of 4.00 ${\mu}M$. The $IC_{50}$ values toward hepatoma Hep G2 and leukemia L1210 cells in vitro were 4.99 ${\mu}M$ and 3.67 ${\mu}M$, respectively.

• Clinical and Experimental Reproductive Medicine
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• v.30 no.4
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• pp.341-350
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• 2003

Objective: The purpose of this study was to identify dietary factors related to infertility in Korean women through a case-control study. Methods: The case group was composed of 236 women who had been diagnosed as infertility in hospital. The control group of 181 healthy women with children were recruited from local immunization centers. Socio-economic status, medical history, dietary intakes using food frequency questionnaire and stress were surveyed by interview. Anthropometric measurements were made and the causes of infertility were identified through medical records. Fasting blood samples were taken from subgroup of the subjects. Results: The mean age of infertile and control groups was 31.1 and 32.4 years, respectively and the difference was statistically significant. The mean Body Mass Index of infertile women was not significantly different from control women, however, Waist/ Hip Ratio and Triceps Skinfolds Thickness were significantly lower in infertile women than in control women. The dietary intake status was generally satisfactory in both groups. The intakes of energy, protein, fat, carbohydrate, retinol, vitamin B2 and niacin were lower in infertile women than in control women. The infertile women also showed lower intakes of animal foods. No differences were found between two groups in serum concentrations of albumin, hemoglobin, Fe, TIBC, total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, C3, IgA, IL-2, however, infertile women showed higher levels of Zn and IgG. The stress score was higher in infertile women. Conclusions: From the results of this study, dietary factors and nutritional status do not seem to be directly related to infertility. However, the intertile women have lower nutrient intake and lower body fat content than control women. Further researches are needed according to the causes of infertility for long term to establish the relationship between dietary factors and infertility.

• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1123-1126
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• 2006

The nutritional composition of Fomitopsis pinicola (F. pinicola) fruiting body has been analyzed for medicinal and edible uses. The contents of crude fibers, carbohydrates, crude protein, moisture, crude fats and ashes were 43.3%, 26.3%, 12.8%, 12.6%, 3.3% and 1.7%, respectively. Eighteen amino acids were found in F. pinicola. Among total amino acids, glutamate content was the highest (457 mg/100 g dry mushroom) and arginine, glycine, valine, aspartate and isoleucine were followed. Concerning free amino acids, glutamine, arginine, trytophan, and glutamate were dominant. The vitamin E content was the highest (276 mg/100 g dry mushroom), then vitamin H and vitamin B, were followed. The mineral contents were as follows: K 165.06 mg, P77.57 mg, Mg 46.11 mg, Fe 21.56 mg, and Ca 16.90 mg based on 100 g dry mushroom.

• Korean Journal of Community Nutrition
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• v.3 no.4
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• pp.594-608
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• 1998

Comparative studies regarding the nutritional status of 93 home-living elderly people taking free congregate lunch meals(FL) and 87 middle income class elderly people(MI) were performed in Taejon city. Data was obtained from questionaires, anthropometry and interviews for the 24-hour dietary recall of 2 nonconsecutive days during August, 1996. The average age for FL was 75.8 years. The monthly familly income for FL belonged to the low-income class. FL females had lower heights and weights than MI females. The average daily nutritional intake of both FL and MI were low, particularly in FL whose %RDA of energy was 68.5%, protein 65%, Ca 29.6%, Fe 50.8%, vitamin A 34.5%, vitamin E 30.5%, riboflavin 40.6%, vitamin C 76.9%. MI's %RDA of energy was 76.4%, protein 80.a2%, Ca 48.1%, Fe 78.6%, vitamin A 67.3%, vitamin E 117.4%, riboflavin 45.6%, vitamin C 136.5%. Comsumption of Zn, vitamin $B_6$ and folic daily average. There was no nutrient having average INQ(Index of nutritional quality) over 1 for either group. The INQs for protein, Ca, Fe and vitamin A were 0.802, 0.377, 0.625 and 0.296 in FL, and 0.900, 0.601, 0.784 and 0.602 in MI, respectively. The MAR(Mean adequacy ratio) was low with the value of 0.500-0.518 in FL and 0.630-0.723 in MI. The percentage of main nutrients from lunch was the highest among the three meals for FL males, while that from breakfast was the highest for MI. Free lunches taken by FL supported higher percentages of main nutrients than home-lunches taken by MI. Eating-out was done more frequently by MI than by FL and that eating-out brought them more nutritional intake. The above data indicated that the dietary nutritional intake status of the FL elderly was very poor in both quantity and quality and that free congregate lunch significantly contributed to the daily nutrient-intake for the FL elderly.

• Nuclear Engineering and Technology
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• v.55 no.5
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• pp.1734-1741
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• 2023

This work presents the synthesis and preparation of a new glass system described by the equation of (70-x) B₂O₃-5TeO₂ -20SrCO₃-5ZnO -xBi₂O₃, x = 0, 1, 5, 10, and 15 mol. %, using the melt quenching technique at a melting temperature of 1100 ℃. The photon-shielding characteristics mainly the linear attenuation coefficient (LAC) of the prepared glass samples were evaluated using Monte Carlo (MC) simulation N-particle transport code (MCNP-5) at gamma-ray energy extended from 59 keV to 1408 keV emitted by the radioisotopes Am-241, Ba-133, Cs-137, Co-60, Na-22, and Eu-152. Furthermore, we observed that the Bi₂O₃ content of the glasses had a significantly stronger impact on the LAC at 59 and 356 keV. The study of the lead equivalent thickness shows that the performance of fabricated glass sample with 15 mol.% of Bi₂O₃ is four times less than the performance of pure lead at low gamma photon energy while it is enhanced and became two times lower the perforce of pure lead at high energy. Therefore, the fabricated glasses special sample with 15 mol.% of Bi₂O₃ has good shielding properties in low, intermediate, and high energy intervals.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.574-579
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• 2010

Waste nutrient solution (WNS) that was the drained nutrient solution of Horticultural Research Institute of Japan for culture tomato in perlite hydroponics showed $1.9-2.4dS{\cdot}m^{-1}$ of EC and 5.7-7.1 pH from April to July. Although ${NH_4}^+-N$ concentration of WNS decreased remarkably, the other nutrients did not change significantly, as compared with supplied solution. There were no significant differences in plant height, stem diameter, and the other growth characteristics of tomato plants grown by 2 fertigation nutrient solutions; BHF (Bountiful Harvest Fertilizer, 10% of N, 13% of $PO_4$, 13% of K, 0.05% of B, 0.05% of Zn, and 0.0023% of Cu that made in Korea) and Megasol (11% of N, 8% of $PO_4$, 34% of K, 0.032% of Mn, 0.002% of B, 0.048% of Fe, 0.0122% of Zn, and 0.0023% of Cu that made in Belgium.); however, the chlorophyll content of tomato leaf was highest in WNS. The fresh and dry weight of tomato plants were higher in 3 fertigation treatments than irrigation of tap water, while there were no significant differences in fresh and dry weight among the 3 fertigation treatments. The mineral content of tomato leaf also did not show any differences among the 3 fertigation treatments and any regular tendency in all minerals. Total yield, fruit weight and fruit numbers of tomato were higher in WNS, followed by Megasol, BHF and control, although there were not any difference among the 3 fertigation nutrient solution treatments. BER(blossom-end rot)of tomato fruits decreased in fertigation treatments, especially, fruits grown in WNS and BHF showed lower BER. However, the transpiration rate of leaf was higher in control, followed by BHF, WNS and Megasol, The fruit size and soluble solids content was higher in 3 fertigation nutrient treatments than control. These results suggest that WNS can be used for fertigation solution in tomato because yield and quality of tomato fruit grown in WNS fertigation treatment were similar to those in 2 fertigation nutrient solutions treatments(BHF, Megasol).

• Journal of Wetlands Research
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• v.23 no.4
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• pp.352-363
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• 2021

The purpose of this study was to evaluate the load of nutrients contained in the drainage discharged from the facility horticultural complex and to use them for re-use of fluids and design for introduction of water treatment plants. Representative hydroponic cultivation crops were selected as tomato, paprika, cucumber, and strawberry, and the total number of samples analyzed for water quality was 80. As a result of the analysis, since various fertilizer components such as N, P, K⁺, Na⁺, Mg²⁺, Ca²⁺, Si₄⁺, HCO₃^-, Cl^-, S^2-, Fe, Mn, Cu, Zn, Mo and B are contained at very high concentrations in the drainage, the need for water treatment was confirmed. Through statistical analysis, it was analyzed that the drainage concentration of strawberries was lower than that of tomatoes, paprika, and cucumbers. In the case of tomatoes, these essential ion concentrations are the highest, so it was confirmed that they are subject to valuable resources in terms of reuse of fertilizers. The load of N and P of the drainage discharged from the facility horticultural complex 1m² was analyzed. For N, the daily processing capacity of 4.0 kg of tomatoes, 3.3 kg of paprika, 3.0 kg of cucumbers, and 1.5 kg of strawberries was calculated based on 1 ha. It was suggested that the P concentration needs a scale and capacity that can handle 0.5 kg of tomatoes, 0.6 kg of paprika, 0.4 kg of cucumber, and 0.2 kg of strawberries per day. Through this study, the amount of nitrogen and phosphorus contained in the drainage discharged from the greenhouse of each crop was evaluated to analyze the economy. In addition, it was expected to be used as basic data that can be used to calculate the treatment capacity to be reflected when introducing water treatment facilities in facility horticultural complexes for sustainable agriculture.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.203-208
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• 1999

An 8 week feeding trial was conducted with 864 ISA Brown laying hens, 48 weeks old, to determine if microbial phytase $(Natuphos^{(R)})$ supplementation can reduce non-phytate phosphorus (NPP) level in laying diets. The experiment consisted of four dietary treatments: T1, control diet with 0.26% NPP (0.55% total P) wand no supplementary phytase; T2, 0.21% NPP (0.50% total P) diet with 250 U of phytase/kg of diet; T3, 0.16% NPP (0.45% total P) diet with 250 U of phytase/kg of diet; and T4, 0.11% NPP (0.40% total P) diet with 250 U of phytase/kg of diet. T3 showed the highest egg production and egg weight and the lowest feed conversion while T4 gave the lowest egg production and the highest feed conversion and mortality. Daily feed consumption ranged from 130.4 g (T4) to 132.7 g (T2). T1 and T2 were not significantly different in the production parameters. Eggshell strength, egg specific gravity, and eggshell thickness were not significantly different among treatments. However, broken egg ratio was significantly lower in T2 and T4 than in T1. Retentions of Ca, P, Mg, and Cu were greater in phytase supplemented treatments (T2, T3, and T4) than the control (T1), and those in T3 and T4 were greater than in T2. Excretions of P in phytase supplemented treatments (T2, T3, and T4) were significantly (p<0.05) smaller than in T1 but excretions of N were not significantly different among the treatments. Contents of ash in tibiae were not significantly affected by treatments, but contents of Ca, P, Mg, and Zn was increased and that of Cu decreased by phytase supplementation. It is concluded that the NPP concentration in the diet of Brown layers consuming about 130 g/d of feed can be safely lowered from 0.26% (0.55% total P) to 0.16% (0.45% total P). The excretion of P was reduced by the inclusion of 250 U phytase/kg of diet.

• Journal of Veterinary Clinics
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• v.21 no.4
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• pp.402-405
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• 2004

A 5-year-old Rottweiler neutered female dog was presented with signs of mandibular and popliteal lymphadenopathy, erosion in mucocutaneous junction of muzzle and lips, multiple papules and nodules in right rear limb and neck, and alopecia in right thorax. There was no further clinical sign except anorexia, sporadic fever and ocular hyperemia. She hadn't shown any response to carprofen prescribed by local veterinarian. Hematological abnormalities included mild anemia and severe lymphocytosis. On serum biochemical profile, only elevated AST level was noticed. On cytological examination, there was an evidence of mild bacterial infection which seemed to occur secondarily. Three sites were biopsied that included muzzle, upper lip and right thoracic region. Histopathologically, multifocal confluent pyogranulomatous dermatitis, scattered granulomatous inflammation in subcutis and severe septal panniculitis were observed. Special stainings(Gram, Acid-fast, PAS, Giemsa) were performed to reveal that a dog was negative for any organism. Finally, sterile pyogranuloma/granuloma syndrome (SPGS) was diagnosed. The treatment was initiated with predinsolone and enrofloxacin. The condition was successfully resolved after 4 weeks of treatment. This good response suggests that SPGS may be immune-mediated disease of its pathogenesis and this drug combination may be a viable therapeutic option for dogs suffering from SPGS. Also, this article reports a case of SPGS in Rottweiller for the first time.

• The Korean Journal of Community Living Science
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• v.16 no.1
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• pp.15-20
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• 2005

To identify and compare the nutritional composition of different kinds of citrus, three examples (Citrus fruit, Kumquat, and Hallabong) were taken from Jeju island in Korea at the same time and their nutritional compositions were measured using the Association of Official Analytical Chemists (AOAC) method. The proximate components (moisture, protein, fat, fiber, ash, and carbohydrates), mineral and vitamin contents were measured. The Nutrient composition revealed some significant differences among the Citrus. The Citrus fruit has more moisture and Fe content than those of the Kumquat. The Kumquat contained more ash, Ca, Na, Mg, Zn, Vitamin A, and B1 than the other Citrus specimens, while the Hallabong had the most protein and Fe. However, fiber, K, Vitamin B₂, C and niacin contents were not significantly different among the specimens. The results demonstrate that the three kinds of citrus used in this study have different nutritional compositions, but they can all be used as a good food for supplementation of K, vitamin A and C. We hope the nutritional analysis of Citrus specimens will be helpful in meeting the consumer's interest in these Citrus, and increase the export of our Citrus produce.