• Asian Pacific Journal of Cancer Prevention
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• v.17 no.5
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• pp.2637-2641
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• 2016

Tumor necrosis factor ($TNF-{\alpha}$), an inflammatory cytokine that plays an important role in the control of cell proliferation, differentiation, and apoptosis, has previously been used in anti-cancer therapy. However, the therapeutic applications of $TNF-{\alpha}$ are largely limited due to its general toxicity and anti-apoptotic influence. To overcome this problem, the present study focused on the effect of active constituents isolated from a medicinal plant on $TNF-{\alpha}$-induced apoptosis in human colon adenocarcinoma (HT-29) cells. Nimbolide from Azadirachta indica was evaluated for cytotoxicity by methyl tetrazolium 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay and phase contrast microscopy. Effects on apoptotic signaling proteins were investigated using Western blot analysis. Nimbolide showed cytotoxicity against HT-29 cells that was significantly different from the control group (p<0.01), a concentration of $10{\mu}M$ significantly inducing cell death (p<0.01). In combination with $TNF-{\alpha}$, nimbolide significantly enhanced-induced cell death. In apoptotic pathway, nimbolide activated c-Jun N-terminal kinase (JNK) phosphorylation, BH3 interacting-domain death agonist (Bid) and up-regulated the death receptor 5 (DR5) level. In the combination group, nimbolide markedly sensitized $TNF-{\alpha}$-induced JNK, Bid, caspase-3 activation and the up-regulation of DR5. Our findings overall indicate that nimbolide may enhance $TNF-{\alpha}$-mediated cellular proliferation inhibition through increasing cell apoptosis of HT-29 cells by up-reglation of DR5 expression via the JNK pathway.

• Journal of the Korean Society for Heat Treatment
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• v.18 no.1
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• pp.18-23
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• 2005

Effect of cold rolling on microstructural changes has been investigated for a Zn-15%Al alloy to elucidate the reason for its work softening behavior. Fully annealed microstructure of the Zn-15%Al alloy is characterized by ${\eta}$ grains and (${\eta}+{\alpha}$) lamellar colonies, where ${\eta}$ and ${\alpha}$ are Zn-rich HCP and Al-rich FCC phases, respectively. The hardness decreases continuously with increasing cold rolling degree, exhibiting work softening behavior. It is revealed that during the cold rolling, (${\eta}+{\alpha}$) lamellar colonies gradually change into equiaxed ${\eta}$ and ${\alpha}$ grains due to dynamic recrystallization at room temperature, while pre-existing ${\eta}$ grains are only deformed without recrystallization. Furthermore, cold rolling causes the precipitation of dissolved Al solutes in ${\eta}$ grains. In view of these results, change of (${\eta}+{\alpha}$) phases from lamellar to equiaxed morphology, which results in structural softness and increase in equiaxed ${\eta}/{\alpha}$ grain boundaries with higher mobility, and deterioration of solution hardening by precipitation of Al solutes from ${\eta}$ grains, are thought to contribute to the work softening of Zn-15%Al alloy.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.15 no.2
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• pp.79-85
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• 2005

Commercial hydroxyapatite (HA) powders were calcined at the temperature range of $1000{\sim}1350^{\circ}C$ in air, for 2h, and the calcined powders were immersed in simulated body fluid (SBF) of pH 7.4 at $37^{\circ}C$ for 3 or 7 days. Thermal decomposition and their related dissolution behaviors of hydroxyapatite were investigated by XRD, FT-IR, and TEM. At the temperature of $1200^{\circ}C$, HA gradually releases its $OH^-$ ions and transforms to OHAP((oxyhydroxyapatite, ($Ca_{10}(PO_4)_6O_x(OH)_{2-2x}$)). HA thermally decomposes to ${\alpha}-TCP$ (${\alpha}-tricalcium$ phosphate) and TTCP (tetracalcium phosphate) phase at $1350^{\circ}C$. It was found that the surface dissolution of the hydroxyapatite powders was accelerated by non-stoichiometric composition and decomposed to ${\alpha}-TCP$ and TTCP.

• Journal of the Korean Chemical Society
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• v.37 no.12
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• pp.1025-1034
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• 1993

We have measured ${\pi}^*$, $\alpha$ and $\beta$ polarity parameters of 2-propanol / hexane and ethyl acetate / hexane mixed solvents over the entire range of composition. For the ethyl acetate / hexane system, only ${\pi}^*$ and $\beta$ were measured since $\alpha$ of these mixed solvents are defined zero. We have corrected the measured polarity parameters to obtain consistent data with the existing literature data assuming a good linear correlation between the measured and the true values. The variation patterns are consistent with the expected trends based on the chemistry of the solvent components. The general trends of the two solvent systems are summarized as follows. ${\pi}^*$ merely increases as the content of the more polar solvent increases while $\alpha$ and $\beta$ increase with the increase of the content of the more polar solvent, then decrease upon continuing addition of the more polar solvent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.2
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• pp.188-191
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• 1985

A thermophilic and cellulolytic bacterium, Herpetosiphon geysericola CUM 317 isolated from the compost, produced ${\alpha}-amylase,\;{\beta}-amylase$, and glucoamylase. Mutual relationships on the production of the three amylases were studied by changing the cultivation conditions. ${\alpha}-Amylase$ and glucoamylase were produced highly after 40 hrs on wheat bran medium at $50^{\circ}C$ and after 30 hrs on liquid medium at $40^{\circ}C$, though ${\beta}-amylase$ was produced best at 10 hrs of initial cultivation phase. The production of the amylases was generally repressed by the addition of carbon sources in liquid medium containing polypeptone. ${\alpha}-Amylase$ production was enhanced relatively by the addition of cupric sulfate in the liquid medium, ${\beta}-amylase$ was enhanced by cadmium sulfate, and glucoamylase was enhanced by calcium chloride.

• Animal cells and systems
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• v.6 no.3
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• pp.277-282
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• 2002

Topoisomearse II is an essential enzyme in all organisms with several independent roles in DNA metabolism. Recently, it has been demonstrated that the C-terminal region of topoisomerases II is associated with hetero-logous protein-protein interactions in human and yeast. In this study, we identified that RTP1, a rat homologue of EIA binding protein BS69, is another topoisomerae II interacting protein by yeast two-hybrid screening. RTP1 has an E1A-binding domain and a MYND motif, which are known to be required for transcriptional regulation by binding to other proteins and interaction with the leucine zipper motif of topoisomerase II. The physical interaction between RTP1 and topoisomerase ll$\alpha$ was examined by GST pull-down assay in vitro. The expression level of RTP1 peaks in S phase as that of topoisomerase ll$\alpha$. These results suggest that the interaction between topoisomerase ll$\alpha$ and RTP1 might play an important role in regulating the transcription of genes involved in DNA metabolism in higher eukaryotes.

• Journal of the Korean Chemical Society
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• v.31 no.4
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• pp.328-333
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• 1987

1-eicosylsulfate($C_{20}H_{41}SO_4^-$) was coated onto 5 ${\mu}m$ C18 reversed phase column. Adsorption equilibrium was reached when 1.2l of $1.46{\times}10^{-4}M$ in 25% acetonitrile solution passed through the column. This coated column proved to be stable up to 10l of 0.05 M ${\alpha}$-HiBA eluent used. The column efficiencies of Eu, Nd, Ce and La measured were about 0.04 mm of HETP using 0.15M ${\alpha}$-HiBA eluent at a flow rate of 1.0ml/min. This column was used to separate some lanthanides and transition metal ions, andmshowed an excellent resolution, respectively.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.5 no.3
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• pp.269-277
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• 1995

Ultrafine TEX>$Al_2O_3$ powders were prepared from $AlCl_3$ and $Al_2(SO_4)_3$3 by using inductively coupled plasma (lCP) of ultrahigh temperature (above 5000 K) in heat source. The prepared $Al_2O_3$ powders had ${\alpha} - group ({\alpha}, {wdelta} ;and; {\theta})$ phase, a narrow size distribution and around 20 nm in meansize. It could be suggested that gas - solid reaction growth and interparticle sintering occured at the center of ICP tail flame (X = 500 mm) through the results of deposited aggregates - flock, whisker and platy on MgO polycrystal plate. And the formation mechanism of $Al_2O_3$ powders In spray - ICP reactor were described from upper results.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2981-2986
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• 2015

Background: Despite many epidemiological studies on the effects of dietary antioxidant micronutrients on risk of cervical cancer, the findings remain uncertain and little evidence is available for serum nutrient markers. The present study aimed to examine the relationship between serum carotenoid, retinol and tocopherol concentrations and risk of cervical cancer among Chinese women. Materials and Methods: We conducted a hospital-based case-control study in which 358 adults (158 incident cases and 200 controls) were recruited from Xinjiang, China. Serum levels of carotenoids (${\alpha}$-carotene, ${\beta}$-carotene, ${\beta}$-cryptoxanthin, lycopene and lutein/zeaxanthin), retinol, and tocopherols (${\alpha}$-tocopherol and ${\gamma}$-tocopherol) were assessed by reverse-phase high-performance liquid chromatography. Results: We found inverse associations between serum carotenoid (${\alpha}$-carotene, ${\beta}$-carotene, and lutein/zeaxanthin) and tocopherol (${\alpha}$-tocopherol) concentrations and the risk of cervical cancer after adjusting for potential confounders, but a null association for retinol. The ORs for 1-SD increase were 0.71 (95 % CI: 0.56-0.92; p=0.003) for total carotenoids and 0.75 (95 % CI: 0.60-0.94; p=0.008) for total tocopherols. Conclusions: These results show that higher serum concentrations of some carotenoids and tocopherols are associated with a lower risk of cervical cancer among Chinese women.

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.340-346
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• 2014

Continuous and dense ZIF-7 membranes were successfully synthesized on ${\alpha}-Al_2O_3$ porous substrate via two-step crystallization technique. ZIF-7 seeding layer was first deposited on porous ${\alpha}-Al_2O_3$ substrate by in-situ low temperature crystallization, and then ZIF-7 membrane layer can be grown through the secondary high-temperature crystallization. Two synthesis solutions with different concentration were used to prepare ZIF-7 seeding layer and membrane layer on porous ${\alpha}-Al_2O_3$ substrate, respectively. As a result, a continuous and defect-free ZIF-7 membrane layer can be prepared on porous ${\alpha}-Al_2O_3$ substrate, as confirmed by scanning electron microscope. XRD characterization shows that the resulting membrane layer is composed of pure ZIF-7 phase without any impurity. A single gas permeation test of $H_2$, $O_2$, $CH_4$ or $CO_2$ was conducted based on our prepared ZIF-7 membrane. The ZIF-7 membrane exhibited excellent H2 molecular sieving properties due to its suitable pore aperture and defect-free membrane layer.