• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.87-95
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• 2007

Many kinds of environment friendly agricultural materials were used for the insect pest control and the control of plant diseases, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Phytoseiulus persimilis might be used for control of two-spotted mites with environment friendly agricultural materials at the same time. For testing the toxicity of environment friendly agricultural materials against to p. persimilis, 61 environment friendly agricultural materials were selected by material contents and using methods. When environment friendly agricultural materials were directly sprayed on P. persimilis, IEFAM C, FEFAM A, EFAMSM A, D, EFAMPE A, EFAMCh B, EFAMME A, and EFAMMo C killed over 90%. However, there was no effects to FEFAM C, D, EFAMSM C, EFAML A, EFAMME C, E, H, J, EFAMMo G and I against P. persimilis. P. persimilis adults were not survived in vial for 48 hours after sprayed and dried with the environment friendly agricultural materials, fer examples, EFAMSM I, EFAMME A, EFAMMo A, C, and I. Otherwise, EFAMCh C and EFAMMo B were no effects to P. persimilis. Some environment friendly agricultural materials are of different qualities, and consequently test of their foxily have to necessary.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.6
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• pp.920-926
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• 2010

Marine alga, Chlorella vulgaris, was extracted by chloroform-methanol (2:1, v/v) solvents for lipid extraction at $35^{\circ}C$ for five hours (HCM-35) and its process was compared with conventional lipid extraction condition such as chloroform-methanol (2:1, v/v) at $65^{\circ}C$ for one hour (CM-65). This low temperature extraction process showed that 80% of total lipid was extracted and its residues contained relatively unchanged amounts of intact proteins and other minerals as well as amino acid profiles. Interestingly enough, the weight fraction of carbohydrate in the residues slightly increased due to less denaturation at low process temperature. The biological activities of the residues such as cytotoxicity and immune cell growth activation were not much changed after being extracted. The sensory evaluation were found to be very favorable for being used as a food additive and/or food supplement. This result could also help to maintain the economic feasibility of utilizing marine resources in food and other relevant industries.

• Development and Reproduction
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• v.4 no.2
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• pp.215-220
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• 2000

Most of endocrine disrupters (EDs) have been reported to exhibit estrogenic or anti-androgenic activity and thereby may disrupt reproductive development in human or wildlife. This study was performed to investigate the effects of estrogen (E$_2$), bisphenol (BP) and octylphenol (OP) on the mouse Leydig cell line (TM3). TM3 originated from testis of 11~13-daly-old BALB/c nu/＋ mice was cultured in DMEM supplemented with 10％ FBS alone or medium with estrogen (E$_2$), bisphenol (BP) and octylphenol (OP; 1 pM, 1 nM, 1 $\mu$M, 1 mM, respectively) for 48 hours. After culture, total cell number and viability were assessed by heamocyto-meter and trypan blue stain. Expression of cytochrome P450scc (CYPscc) mRNA whose product is involved in steroid hormone biosynthesis and estrogen receptor $\alpha$(ER $\alpha$) mRNA were detected by RT-PCR. As a result, treatment of TM3 with E$_2$, BP and OP(1 mM, respectively) significantly decreased the viability but not all of groups as high as 1 $\mu$M. Exposure of TM3 to OP significantly reduced the total cell number but not E$_2$ or BP. The expression of CYPscc mRNA was slightly reduced in BP (1 nM, 1 $\mu$M) and significantly decreased in OP (1 nM, 1 $\mu$M) treated TM3, except E$_2$ group. But the expression of ER $\alpha$ mRNA was sightly increased in all treated groups. In conclusion, BP and OP (high concentration) might inhibit steroidogenesis by decreasing the CYPscc mRNA expression in the mouse testis. These results suggest that BP and OP might impair spermatogenesis and subsequently disturb testicular function.

• Journal of Food Hygiene and Safety
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• v.29 no.3
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• pp.195-201
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• 2014

This study was conducted to investigate the current status of hygiene performance and food preparation/storage condition in restaurants during the summer season in order to evaluate the sanitary management systems in restaurants against climate change. Total 30 restaurants located in Gyeonggi participated in a survey in which they were asked current hygiene performance, food preparation/storage condition, and purchasing practices for 5 food ingredients. As results, regarding the performance degree of respondents on food hygiene management, the average scores of 9 questions were well over 4 points. However, only 6.83% of the respondents claimed that they use sanitizers (chlorine) to disinfect food ingredients. About food storage condition, a high proportion of respondents said that they store food materials in plastic bags or airtight containers following pretreatment and use refrigerator for the storage of pretreated food materials. However, 5.55% and 14.85% of respondents answered that they store pretreated food materials in the kitchen or inside of dining room, respectively. Respondents (21.50%) answered that they store pretreated food materials for more than 6 hours before cooking. Therefore, food materials need to be disinfected properly with sanitizer to remove microbial contamination and stored at refrigerator using closed bags or containers before cooking in order to prevent foodborne disease in restaurants especially during summer season.

• Clinical and Experimental Pediatrics
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• v.51 no.6
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• pp.597-603
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• 2008

Purpose : Obesity is associated with insulin resistance. Insulin resistance and the presence of pro-inflammatory mediators are thought to cause a state of vascular endothelial dysfunction, an abnormal lipid profile, hypertension, and vascular inflammation. These chronic inflammatory responses, which are characterized by abnormal cytokine production, lead to activation of a pro-inflammatory signaling pathway. Leptin is an important mediator of inflammatory processes and immune-mediated diseases. The purpose of this study was to investigate the relationship between leptin and various cytokines associated with obesity in adolescents. Methods : Sixty-six obese adolescents (between 16-17 years of age, obesity index >130%) and 26 normal controls were included in this study. Obesity index and body mass index (BMI) were calculated. Serum lipid profile, AST and ALT were tested after 10 hours of fasting. Tumor necrosis factor alpha (TNF-${\alpha}$) and Interleukin-6 (IL-6) levels were measured by ELISA. Insulin, adiponectin, and leptin levels were estimated by radioimmunoassay. Results : Leptin was significantly higher in the obese adolescents compared to the control adolescents ($12.0{\pm}6.8ng/mL$ vs $6.3{\pm}1.0ng/mL$). TNF-${\alpha}$, IL-6, and insulin were significantly higher in the obese adolescents. Adiponectin was significantly lower in the obese group than the control group ($3.3{\pm}1.9{\mu}g/mL$ vs $5.0{\pm}1.4{\mu}g/mL$). Leptin had positive correlations with obesity index, BMI, and IL-6. Conclusion : In obese adolescents, leptin, TNF-${\alpha}$, IL-6, and insulin might be important mediators of obesity. Further clinical research is necessary to ascertain leptin as a predictor of cardiovascular diseases and to develop a guideline for clinical intervention.

• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.261-267
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• 2007

The acute toxicity of several pesticides on 4 Korean water flea was investigated to develop a new standard species used for ecological risk assessment of pesticide. Four Korean freshwater cladocerans, Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocepholus vetulus were exposed to five different pesticides during 48 hours to compare their sensitivity with a standard test species, Daphnia magna, endorsed formally by the major international organizations. The synthetic pyrethroid, fenpropathrin was the most toxic pesticide to cladocerans. Diazinon, carbofuran, iprodione and myclobutanil were in the order of their toxicity to cladocerans tested. There was no consistent difference in sensitivity to five pesticides for four Korean cladocerans tested. In conclusion, the ecological risk assessment using single species toxicity referred to base set data should not be enough to protect to every species in the field environment.

• Clinical and Experimental Pediatrics
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• v.52 no.10
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• pp.1140-1146
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• 2009

Purpose:The pathophysiology of magnesium, the second highest common compound in humans, is still unclear, especially in preterm babies. We accessed the association between total magnesium (tMg), ionized Mg (iMg), and gestational age (GA) and that between serum magnesium (sMg) and intraventricular hemorrhage (IVH) in preterm babies. Methods:In all, 119 inborn preterm infants admitted between July 2006 and February 2008 were divided into the IVH group (19) and the control group (100) and were prospectively analyzed. TMg, iMg, pH, total calcium (tCa), and ionized Ca (iCa) levels were determined immediately after delivery or within 3 hours after birth, and their correlation with GA were investigated. Results:TMg was not correlated with GA, tCa, iCa, and pH. IMg was correlated with tMg (r=0.288, P=0.002) and iCa (r=0.212, P=0.021); however, it was not correlated with GA and pH. Mean GA and birth weight were significantly lower (P=0.002) and smaller (P=0.030) in the IVH group. Mean sMg was higher in the IVH group ($2.5{\pm}0.9mg/dL$) than in the control group ($2.1{\pm}0.6mg/dL$) (P=0.021). SMg was a risk factor even after logistic regression analysis (OR, 2.798; 95% C.I., 1.265-6.192; P=0.011). Conclusion:In less than 37-week-old preterm babies, tMg and iMg were similar, regardless of GA. High sMg may be a risk factor for IVH in premature babies, regardless of their exposure to antenatal magnesium.

• Journal of agricultural medicine and community health
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• v.38 no.4
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• pp.229-242
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• 2013

Objectives: The objective of this study was to identify the influence of shift work on sleep quality, daytime sleepiness, and perceived errors during nursing work among hospital nurses. Methods: The study subjects were 209 hospital nurses working in two general hospitals in South Korea. The data were collected through self-administered questionnaires in May 2013. Sleep quality was measured using Korea Pittsburgh Sleep Quality Index (PSQI-K), daytime sleepiness was measured using Epworth Sleepiness Scale (ESS), and perceived errors in past 6 months during nursing duty hours with the tool developed in this study. Results: The sleep quality was significantly worse among rotating shift nurses when compared to fixed day duty nurses. However, the odds ratio (OR) of 1.92 [95% confidence interval(CI): 0.58-6.37] adjusted for sociodemographic variables, health behaviors, and working environment in logistic regression was not significant. There was no significant difference in the daytime sleepiness between rotating shift nurses and fixed day duty nurses in both simple and multivariable analyses. There were significantly more perceived errors in rotating shift nurses than fixed day duty nurses. However, the OR of 1.26 (95% CI: 0.38-4.21) adjusted for sociodemographic variables, health behaviors, working environment, sleep quality, and daytime sleepiness in logistic regression was not significant. Depression (OR=3.31, 95% CI: 1.11-9.84) and daytime sleepiness(OR=1.18, 95% CI: 1.07-1.30) were significantly associated with perceived errors in logistic regression. Conclusion: This study suggested shift work among hospital nurses had no independent influence of sleep quality, daytime sleepiness, and perceived errors. However, daytime sleepiness had independent influence of perceived errors. More studies are warranted to achieve more reliable conclusion.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.3
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• pp.391-399
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• 2004

The purpose of this study was to compare curing efficiency of newly developed curing units to traditional halogen curing unit by measuring thermal change and surface microhardness according to curing light system. Materials and mathods : The types of curing units were traditional low intensity halogen light(Optilux 360), plasma arc light(Flipo), low heat plasma arc light(Aurys), low intensity LED(Starlight), and high intensity LED(Freelight2). Temperature at the tip of light guide was measured by a digital thermometer using K-type thermocouple. And after resin was filled to 2, 3, 4mm teflon mold, bottom temperature measured during curing. After 24 hours, microhardness of top surface and bottom surface of each resin specimen were measured. Results : The result of this study can be summarized as follows, 1. As measuring temperature of curing unit tips, Flipo is the highest as $52.4^{\circ}C,\;Freelight2(37.86^{\circ}C),\;Optilux360(32.68^{\circ}C),\;Aurys(32.34^{\circ}C),\;and\;Starlight(26.14^{\circ}C)$ were followed. 2. Flipo and Freelight2 were the highest similarly and Optilux360 and Aurys were similarly next and Starlight was lowest in temperature of bottom surface of resin mold. 3. Microhardness of top surface were generally similar, and Aurys was relatively low. 4. Optilux 360 and Freelight2 were the highest, and Flipo, Starlight, and Aurys were followed in microhardness of bottom surface. Conclusions : The results suggest that careful use of Flipo and Freelight2 might be able to cure greater depth of resin composite and do not cause thermal problems than other curing units.

• Journal of Animal Environmental Science
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• v.9 no.2
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• pp.93-102
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• 2003

This study designed and constructed an experimental column far adhesion efficiency test and conducted experiment to investigate the offensive odor adhesion efficiency of filter bed materials. The offensive odor adhesion experiment was conducted using mixture of high physical adhesion efficiency material, and the fixity of deodorization microorganism of selected filter bed material was tested using ammonia exclude microorganism A4-­2 and sulfur oxidation microorganism S5­-5.2 those were cultured at the Agricultural Chemical Department of Chungnam National University, and deodorization efficiency of selected filter bed material mixture was tested. Following are summary of these tests results. 1. Amount of elimination of the offensive odor gas of ammonia and hydrogen sulfide per unit volume was 0.054 and 0.016$\ell/\textrm{cm}^3$ in rice hull, 0.01 and 0.004 $\ell/\textrm{cm}^3$ in rice straw 0.158 and 0.01 $\ell/\textrm{cm}^3$ in coconut, 0.014 and 0.02$\ell/\textrm{cm}^3$ perlite, 0.004 and 0.003$\ell/\textrm{cm}^3$ in high road ball, and 0.112 and 0.015 $\ell/\textrm{cm}^3$ in chaff of pine, respectively. 2. Amount of elimination of offensive odor gas of ammonia and hydrogen sulfide per unit volume was 0.045 and 0.014$\ell/\textrm{cm}^3$ in mixture 1, 0.079 and 0.016$\ell/\textrm{cm}^3$ in mixture 2, 0.123 and 0.017 $\ell/\textrm{cm}^3$ in mixture 3, 0.031 and 0.015$\ell/\textrm{cm}^3$ in mixture 4, 0.055 and 0.016$\ell/\textrm{cm}^3$ in mixture 5, and 0.111 and 0.020$\ell/\textrm{cm}^3$ in mixture 6, respectively. 3. The offensive odor elimination microorganism inoculated to mixture of chaff of pine(70%) and perlite(30%) showed the elimination efficiency of 99.06% and 96.61% against the ammonia and hydrogen sulfide, respectively, during 24 hours period.