This study aimed to investigate the level of public service motivation among health-related college students and to identify differences according to general characteristics as well as influencing factors. A descriptive survey design was employed, and the participants consisted of 314 students enrolled in universities located in the Gyeongnam region. Data were collected using a structured questionnaire and analyzed using SPSS version 29.0. Statistical analyses included frequency, percentage, mean, standard deviation, independent t-test, one-way ANOVA with Duncan post-hoc test, and multiple linear regression analysis. The results showed that the mean score of public service motivation was 3.81±0.699, indicating a moderate to high level overall. Significant differences in public service motivation were observed according to age, department, and academic year. In particular, students in the emergency medical technology department showed significantly higher levels of public service motivation compared to students in the biomedical health-related department. In the multiple regression analysis, department was identified as the only significant predictor of public service motivation, whereas gender, age, religion, academic year, and having a family member in the medical field were not statistically significant factors. The explanatory power of the regression model was relatively low, suggesting that public service motivation is a complex construct influenced by various psychological, educational, and social factors. These findings indicate that public service motivation among health-related college students differs according to certain general characteristics, particularly academic major. Therefore, it is necessary to develop educational programs and curricula tailored to the characteristics of each major in order to strengthen public service motivation and encourage its translation into actual professional and social behaviors.