This study aimed to examine the overall level of psychological burnout among healthcare college students and to identify differences according to general characteristics, as well as factors influencing burnout. A descriptive cross-sectional survey design was employed. The participants consisted of 183 healthcare students enrolled at a university in the Gyeongnam region, and data were collected using a structured questionnaire. The collected data were analyzed using SPSS version 29.0, including descriptive statistics, independent-samples t-tests, one-way analysis of variance, and multiple linear regression analysis. Differences in psychological burnout according to general characteristics were examined. The results showed that the mean score of psychological burnout was 2.48, indicating a level below moderate. Among the survey items, "feeling tired when thinking about going to school in the morning" showed the highest mean score (M = 3.30). In terms of gender differences, female students (M = 2.57) reported significantly higher levels of psychological burnout than male students (M = 2.28) (t = 7.3 17, p < .01). Significant differences were also observed across academic departments (p < .01), with students in the Department of Companion Animal Science showing higher levels of burnout compared to other majors. Multiple linear regression analysis revealed that the overall model was statistically significant (F = 3 .29, p < .05). Gender (β = .161, p = .027), academic department (β = .236, p = .008), and year of study (β = .318, p = .010) were identified as significant predictors of psychological burnout, with an adjusted explanatory power of 8.1% (Adjusted R2 = .081). These findings suggest that psychological burnout among healthcare college students can be understood as a result of the combined effects of individual characteristics and educational environmental factors. Therefore, it is necessary to develop differentiated psychological support and educational intervention strategies that consider students' academic departments and year of study.