The purpose of this study was to identify the effect of experience emotion (i.e., happy, proud, sad, fear) on the risk perception. This study also examined interaction effects of emotion regulation (i.e., reappraisal strategy, problem focused strategy) between experience emotion and risk perception. The study collected data from 168 flight crew members in Korean commercial airlines, using an online research in which an experiment of emotion manipulation and a survey were included. The results of the study found the positive effect of happiness emotion on the risk perception regarding cases 1(these cases have high possibility of negative result and low circumstance control) and the positive effect of sadness emotion on the risk perception regarding cases 2(these cases have low possibility of negative result and high circumstance control). This study also found the interaction effect of reappraisal emotion regulation strategy between the relationship of happiness and risk perception regarding cases 2. From these results, the study provided that theoretical and practical implication that happiness and sadness emotion contribute risk perception and reappraisal strategy has a moderating role in the relationship between happy emotion and risk perception. Finally, based on these results, the limitations of this study and future research were discussed.