Purpose: Lower limb coordination plays a vital role in determining balance, walking speed, and functional mobility in individuals with stroke. This study aimed to evaluate the inter-rater and test-retest reliability, as well as measurement error indices-including the standard error of measurement (SEM) and minimal detectable change (MDC)-of the Lower Extremity Motor Coordination Test (LEMOCOT) in patients with subacute stroke. Additionally, the study examined the construct validity of LEMOCOT. Methods: A cross-sectional study was conducted with 30 individuals in the subacute phase of stroke recovery. Inter-rater and test-retest reliability were assessed using the intraclass correlation coefficients (ICC2,1 and ICC3,1, respectively). Measurement error was calculated using SEM, MDC, and MDC (%). Bland-Altman plots were used to analyze the 95% limits of agreement between paretic and nonparetic limb scores. The construct validity of LEMOCOT was determined by correlating performance with scores from the Fugl-Meyer Assessment-Lower Extremity (FMA-LE), Berg Balance Scale (BBS), modified Four Square Step Test (mFSST), 10-Meter Walk Test (10mWT), Timed Up and Go Test (TUG), and 6-Minute Walk Test (6MWT) using Spearman's correlation coefficient. Results: LEMOCOT demonstrated excellent inter-rater reliability (ICC = 0.925, paretic; 0.880, nonparetic) and high test-retest reliability (ICC = 0.906, paretic; 0.808, nonparetic). MDC values indicated minimal measurement error. Strong correlations were found between LEMOCOT performance and functional outcomes, such as FMA-LE (r = 0.734-0.749) and BBS (r = 0.587-0.593), as well as moderate to strong correlations with mobility tests, including the 10mWT, TUG, and 6MWT. Conclusion: LEMOCOT is a reliable and valid clinical tool for assessing lower extremity coordination in patients with subacute stroke. Its simplicity and efficiency support its use in routine rehabilitation settings.