Purpose: The aim of this study was to apply and compare the effects of warm-up and rewarm-up on the skill-related fitness of amateur soccer players and determine how to effectively improve skill-related fitness. Methods: Twenty-five males with at least 5 years of soccer experience, including middle and high school sport club activities, and participated in games at least once a week voluntarily participated in the study. The warm-up measurements were conducted in the order of pre-measurement, warm-up, 6-minute rest, and post-measurement, and the rewarm-up measurements were conducted in the order of pre-measurement, warm-up, 6-minute rest, rewarm-up, and post-measurement. To prevent learning effects, the warm-up and rewarm-up conditions were changed at one-week intervals. Results: First, all skill-related aspects of fitness (agility, quickness, speed, and coordination) showed a significant increase in within-condition differences according to the training program. Second, there was an interaction effect according to the training program between agility, quickness, and speed. Third, there was no interaction effect according to the training program for coordination. Therefore, both warm-up and rewarm-up programs had a positive effect on skill-related fitness. Conclusion: In particular, agility, quickness, and speed showed greater improvement when rewarm-up was performed. This suggests that soccer players should perform both warm-up and rewarm-up during pregame breaks to improve their performance.