Purpose: To identify factors influencing performance in end-of-life care, our study aimed to investigate the attitude, and end-of-life care stress of intensive care unit (ICU) nurses. Methods: We conducted a cross-sectional study using a self-administered questionnaire from March 6 to March 20, 2023 among 180 ICU nurses at A university hospital in Suwon city, Gyeonggi-do, South Korea. The data were analyzed for descriptive statistics (frequency, percentage, mean, standard deviation), independent sample t-tests, one-way ANOVA, Pearson's correlation coefficient, and multiple regression analysis using the IBM SPSS Statistics version 29.0. Results: The mean scores for end-of-life care stress, attitude, and performance were 142.11 ± 22.97, 80.95 ± 6.67, and 58.01 ± 7.95, respectively. End-of-life care performance was positively correlated with end-of-life care stress (r = .28, p < .001) and attitude (r = .28, p < .001). There were significant differences based on gender (t = -2.06, p = .041) and education (t = -2.33, p = .021) in end-of-life care performance. Multiple regression analysis revealed that end-of-life care stress (𝛽 = .18, p = .035), attitude (𝛽 = .17, p = .043), and education background (𝛽 = .16, p = .031) were significant predictors of end-of-life care performance, explaining 11.7% of the variance. Conclusion: Our findings suggest that strategies to manage stress, enhance positive attitudes, and provide education on end-of-life care are essential to improve the end-of-life care performance of ICU nurses.