Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory Activity of Ethanol Extract from Vaccinium bracteatum Leaves in RAW 264.7 Cells

RAW 264.7 세포에서 모새나무 잎 에탄올 추출물의 항염증 활성 평가

  • Choyeon Lee (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Yeongha Kim (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Chae Eun Woo (Division of Cosmetics, Daegu Haany University) ;
  • Jihye Lee (Industry Academic Cooperation Foundation, Daegu Haany University) ;
  • Ji Wook Jung (Department of Cosmetics, Daegu Haany University)
  • 이초연 (대구한의대학교 제약공학과) ;
  • 김영하 (대구한의대학교 제약공학과) ;
  • 우채은 (대구한의대학교 화장품학부) ;
  • 이지혜 (대구한의대학교 산학협력단) ;
  • 정지욱 (대구한의대학교 화장품학과)
  • Received : 2026.03.09
  • Accepted : 2026.04.06
  • Published : 2026.05.31
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Abstract

Objectives : This study aimed to evaluated the anti-inflammatory activity of the 70% ethanol extract from Vaccinium bracteatum leaves to investigate its effects on inflammatory mediators. Methods : Cell viability was assessed using an MTT assay in RAW 264.7 macrophages. Inflammatory responses were induced by lipopolysaccharide (LPS), and nitric oxide (NO) production was measured. The expression levels of inducible nictric oxdie synthase (iNOS) and cyclooxygenase-2 (COX-2) were analyzed by Western blotting, while interleukin-6 (IL-6) production was quantified using ELISA. Results : The ethanol extract exhibited no significant cytotoxicity up to 300 ㎍/mL. In LPS-stimulated RAW 264.7 cells, NO production was significantly inhibited in a concentration-dependent manner. In addition, the expression of iNOS and COX-2 proteins was markedly reduced, and IL-6 production was significantly suppressed following extract treatment. Conclusions : The ethanol extract of V. bracteatum leaves exhibited excellent anti-inflammatory activity by suppressing the production of NO and the expression of key inflammatory mediators, including iNOS, COX-2, and IL-6. These findings suggest its potential as a natural anti-inflammatory functional material.

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Acknowledgement

본 결과물은 2025년 교육부 및 경상북도의 재원으로 경북 RISE센터의 지원을 받아 수행된 대구한의대학교 지역혁신중심 대학지원체계(RISE) 글로컬대학30사업-(특화분야별 산학공동기술개발과제)의 결과입니다. (2025-RISE-15-110)