Animal Bioscience

  • 제38권12호
  • /
  • Pages.2584-2596
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  • 2025
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Transcriptional regulation of porcine PABPN1 gene in adipogenesis

  • Rong Ru Zhu (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Xue Lian Zhao (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Ming Hang Chang (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Si Qi Yang (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Xiao Han Zhang (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Ying Ke Liu (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Zhi Gang Gu (College of Animal Science and Technology, Northeast Agricultural University) ;
  • Xiu Qin Yang (College of Animal Science and Technology, Northeast Agricultural University)
  • 투고 : 2025.01.17
  • 심사 : 2025.06.20
  • 발행 : 2025.12.01
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초록

Objective: This study aims to reveal the transcriptional regulatory mechanism and effects of poly(A)-binding protein nuclear 1 (PABPN1) on adipogenesis, along with associated polymorphisms. Methods: Transcription factors were identified using dual-luciferase reporter assay, over-expression techniques site-directed mutagenesis, real-time quantitative polymerase chain reaction (qPCR), electrophoretic mobility shift assays and chromatin immunoprecipitation-qPCR. Preadipocyte differentiation was measured with gain- and loss-of-function, Oil Red O staining and extraction assays. Single nucleotide polymorphisms (SNPs) were identified with direct sequencing of PCR products in the promoter, and the effects of these SNPs on PABPN1 expression were identified with dual-luciferase reporter assay. Results: CCAAT/enhancer-binding protein (C/EBP) α and β regulate PABPN1 expression by directly binding to its promoter. PABPN1 promotes the preadipocyte differentiation in pigs. Three SNPs were identified, with the Haplotype GCC mutation significantly increasing the promoter activity of PABPN1. Conclusion: PABPN1 promotes the preadipocyte differentiation as a downstream gene of C/EBP α and β. Haplotype GCC may serve as a molecular marker for selection of fat traits in pigs.

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과제정보

This research was funded by the National Natural Science Foundation of China (32472870) and Key Research and Development Program of Heilongjiang Province (No. 2022ZX02B10-1).