Animal Bioscience

  • 제38권12호
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  • Pages.2545-2557
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  • 2025
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Analysis of circular RNA (circRNA) characteristics and identification of key circRNAs in the hypothalamus during sexual maturation in female goats

  • Qing Li (Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Jianmin Wang (Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Yanyan Wang (Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Peipei He (Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Lu Zhang (Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Tianle Chao (Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University)
  • 투고 : 2025.04.15
  • 심사 : 2025.06.09
  • 발행 : 2025.12.01
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초록

Objective: Precocious puberty can shorten reproductive cycles, enhance reproductive capacity, and reduce feeding costs. Consequently, precocious livestock are widely utilized in cross-breeding. This study aims to elucidate the key molecular mechanisms by which circular RNA (circRNA) regulates the sexual maturation of sexually precocious goats. Methods: In this study, we analyzed the circRNA expression profiles of hypothalamic tissue from Jining grey goats at four distinct postnatal developmental stages (1 day, 2 months, 4 months, 6 months). Results: A total of 23,993 circRNAs were identified across these stages, predominantly derived from exonic regions, with 1,052 circRNAs exhibiting differential expression. Additionally, competitive endogenous RNA (ceRNA) analysis suggested that novel_circ_0002274/chi-miR-197-5p/ estrogen receptor 1 (ESR1) and novel_circ_0002274/chi-miR-30c-3p/ oxytocin/neurophysin I prepropeptide (OXT) may regulate sexual maturation in goats via the estrogen signaling pathway and the oxytocin signaling pathway. Conclusion: This study contributes to understanding the function of circRNAs in hypothalamic regulation of sexual maturation and provides valuable insights for breeding superior goat breeds.

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과제정보

This project was financed by the Project of Improved Agricultural Varieties in Shandong Province (2021LZGC010), The National key R & D plan (Integration and Demonstration of Key Technologies for Large-scale, Efficient and Safe Breeding of Goats) (2018YFD0501906), Natural Science Foundation project of Shandong Province (ZR2023QC044), Shandong Provincial Sheep and Goat Industry Technology System (SDAIT-10-01), Shandong "Double Tops" Program (SYL2017YSTD12), and The Project of Improved Agricultural Varieties in Shandong Province (2019LZGC012).

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