Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Fractionation of Essential Oils Extracted from Litsea angulata (Lauraceae) Using Two Different Methods and Their Impact on Lactic Acid Bacteria

  • Agmi Sinta PUTRI (Laboratory of Forest Product Chemistry and Renewable Energy, Faculty of Forestry and Tropical Environment, Mulawarman University) ;
  • Irawan Wijaya KUSUMA (Laboratory of Forest Product Chemistry and Renewable Energy, Faculty of Forestry and Tropical Environment, Mulawarman University) ;
  • Najmia Afifah ZULFA (Laboratory of Forest Product Chemistry and Renewable Energy, Faculty of Forestry and Tropical Environment, Mulawarman University) ;
  • Rita DIANA (Laboratory of Ecology and Tropical Forests Biodiversity Conservation, Faculty of Forestry and Tropical Environment, Mulawarman University) ;
  • Sarifah NURJANAH (Department of Agriculture Engineering and Biosystem, Padjadjaran University) ;
  • Kosei YAMAUCHI (United Graduate School of Agriculture Sciences, Gifu University) ;
  • Harlinda KUSPRADINI (Laboratory of Forest Product Chemistry and Renewable Energy, Faculty of Forestry and Tropical Environment, Mulawarman University)
  • Received : 2025.05.20
  • Accepted : 2025.07.20
  • Published : 2025.09.25
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Abstract

This study was conducted to analyze the physicochemical characteristics of leaf essential oil fractions from Litsea angulata and evaluate their antibacterial activity against lactic acid bacteria. Essential oils were extracted and fractionated using two methods: simple fractional (SF) and spinning band (SB) distillation. Four bacterial strains were tested: Streptococcus mutans, Streptococcus sobrinus, Staphylococcus aureus, and Lactobacillus casei. The highest yields were obtained from fractions SF1 (0.83%, w/w) and SB6 (0.53%, w/w), which showed distinct color variations. Gas chromatography-mass spectrometry analysis revealed that monoterpenes, particularly α-phellandrene (> 40%), were the dominant components, followed by sesquiterpenes and other compounds. Fractions SF1 and SB6, which are rich in monoterpenes, exhibited the highest antibacterial activity, surpassing that of the positive control against S. mutans, S. sobrinus, and S. aureus. In contrast, SF2 (rich in sesquiterpenes, 33.36%) was more active against L. casei. These results suggest that L. angulata is a promising natural source of α-phellandrene and shows potential for development as an antibacterial agent, particularly for oral and dental health applications.

Keywords

Acknowledgement

This project was funded by the Program Hibah Islamic Development Bank (IDB) 2018 (No. 448/UN17.45/DL/2018) and the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia (Grant No. 221/UN17.41/KL/2019).

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