Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Optimization of Extraction Conditions for Functional Ginsenosides in Gynostemma pentaphyllum Leaves

돌외 잎의 기능성 진세노사이드 성분에 대한 추출 조건 최적화

  • Do Hyeong Kim (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Jun Hwi Ko (Department of Global Innovative Drugs, Chung-Ang University) ;
  • Hyojin Lee (Department of Biochemical Engineering, Seoil University) ;
  • Ji Hoon Jeong (Department of Global Innovative Drugs, Chung-Ang University) ;
  • Byung Wook Yang (Department of Beauty Cosmetics, Osan University)
  • 김도형 (세명대학교 한방식품영양학과) ;
  • 고준휘 (중앙대학교 글로벌혁신신약학과) ;
  • 이효진 (서일대학교 생명화학공학과) ;
  • 정지훈 (중앙대학교 글로벌혁신신약학과) ;
  • 양병욱 (오산대학교 뷰티코스메틱계열)
  • Received : 2025.05.05
  • Accepted : 2025.06.14
  • Published : 2025.06.30
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Abstract

Objectives: The aims of this study was to evaluate the ginsenoside content in the leaves of Gynostemma pentaphyllum (Cucurbitaceae), under different extraction conditions. As a dammarane triterpene-type derivative, G. pentaphyllum contains ginsenosides similar to those in ginseng, making it a potential source for herbs as functional food ingredients with anti-obesity effects. Methods: Ginsenosides were isolated from G. pentaphyllum extracts using both water and ethanol at different concentrations and duration. Water extractions were performed at 100 ℃ for 10 and 24 hours. Solvent extractions used 50%, 70%, and 95% ethyl alcohol. Ginsenoside contents were analyzed in each extract by high performance liquid chromatography. Results: Water extraction of G. pentaphyllum leaves at 100 ℃ for 24 hours (GPW-24) showed the highest total content of ginsenoside. However, 10-hour extraction (GPW-10) was yielded the highest concentrations of ginsenoside Rh1 (0.886%) and Rc (0.566%). In solvent extraction, total ginsenosides were highest in 95% ethanol, and were followed by 50%, 70%, and water. Ginsenoside Rc was abundant in both 95% (4.745%) and 50% ethanol (4.769%) extracts. Conclusions: The optimal water extraction condition for obtaining functional ingredients is 10-hour extraction at 100 ℃. The best solvent extraction condition is using 95% ethanol, suggesting effective strategies for utilizing G. pentaphyllum in functional herbal ingredients with anti-obesity effects.

Keywords

Acknowledgement

이 연구는 2025학년도 오산대학교 교내연구비 지원에 의하여 이루어졌음.

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