International Journal of Oral Biology

  • 제50권2호
  • /
  • Pages.64-73
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  • 2025
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Antioxidative and anti-inflammatory effects of the ethanol extract of Salvia plebeia R. Br. on human gingival fibroblast cells

  • 투고 : 2025.04.15
  • 심사 : 2025.05.29
  • 발행 : 2025.06.30
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초록

Periodontitis is a chronic inflammatory condition primarily triggered by bacterial infections, with periodontopathogens such as Porphyromonas gingivalis playing a pivotal role. We evaluated the antioxidant and anti-inflammatory effects of ethanol extract of Salvia plebeia R. Br. (SP-E) on human gingival fibroblasts (hTERT-hNOF) stimulated with P. gingivalis-derived lipopolysaccharide (LPS). Dried S. plebeia was extracted using 70% ethanol, yielding a 10.5% extract. Inflammation in hTERT-hNOF cells was induced using P. gingivalis LPS in conjunction with LPS-binding protein and CD14. SP-E was administered at concentrations ranging from 25 to 100 ㎍/mL. Antioxidant capacity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) activity assay. Inflammatory cytokine expression and secretion were analyzed via reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results demonstrated a concentration-dependent antioxidant effect, with 62.98% radical scavenging activity observed at 200 ㎍/mL SP-E. In hTERT-hNOF cells, SOD activity increased from 4.88% (LPS-treated) to 45.78% with 100 ㎍/mL SP-E. RT-PCR analysis showed significant downregulation of interleukin (IL)-1β, IL-6, and IL-8 mRNA expression following SP-E treatment. ELISA confirmed a reduction in tumor necrosis factor (TNF)-α (312.83 → 178.22 pg/mL), IL-6 (453.97 → 170.83 pg/mL), and IL-8 (480.14 → 276.86 pg/mL) levels with 100 ㎍/mL SP-E. These findings suggest that SP-E may offer therapeutic potential for preventing and managing periodontal disease by mitigating oxidative stress and modulating inflammatory cytokine expression. Further studies are warranted to elucidate the underlying molecular mechanisms and validate these effects in vivo.

키워드

과제정보

이 성과의 일부분은 정부(중소벤처기업부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. RS-2023-00224207).

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