BMB Reports

  • 제58권6호
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  • Pages.238-243
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  • 2025
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  • 1976-6696(pISSN)
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  • 1976-670X(eISSN)
생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Tat-GSTpi suppresses inflammatory responses by regulating ROS/MAPKs/apoptosis signaling pathways

  • Eun Ji Yeo (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Min Jea Shin (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Gi Soo Youn (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Jung Hwan Park (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Hyeon Ji Yeo (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Hyun Jung Kwon (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Yeon Joo Choi (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Lee Re Lee (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Na Yeon Kim (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Su Yeon Kwon (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Su Min Kim (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Keun Wook Lee (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Jong Kook Park (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Chan Hee Lee (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Dae Won Kim (Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Duk-Soo Kim (Department of Anatomy, College of Medicine, Soonchunhyang University) ;
  • Won Sik Eum (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Soo Young Choi (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
  • 투고 : 2024.07.22
  • 심사 : 2024.10.01
  • 발행 : 2025.06.30
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초록

Glutathione S-transferase pi (GSTpi) is a phase II detoxifying enzyme that plays key roles in cellular processes. In a previous study, we have reported that cell permeable Tat-GSTpi can protect dopaminergic neurons against cell death. However, the precise roles of GSTpi in inflammation remain to be elucidated. Thus, the objective of present study is to investigate the one of plausible protective mechanism involved anti-inflammatory effect of GSTpi using lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced macrophages and an animal model. It was revealed that cell permeable Tat-GSTpi fusion protein markedly reduced reactive oxygen species (ROS) and DNA injury in LPS-treated cells and transduced protein showed not only inhibition of the regulation of mitogen-activated protein kinase (MAPK) and Caspase-9, but also decrease of COX-2 and iNOS expressions. Furthermore, Tat-GSTpi ameliorated skin inflammation in an animal model by inhibition the COX-2, iNOS expression and cytokines. Those results indicate that GSTpi plays a role in antagonizing LPS- and TPA-induced inflammation, suggesting GSTpi has the potential to serve as a therapeutic treatment for inflammatory related diseases.

키워드

과제정보

This research was supported by Basic Science Research Program (2019R1A6A 1A11036849) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education.

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