대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제51권1호
  • /
  • Pages.85-93
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  • 2025
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
권호 표지
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MAPK/Akt 신호 경로를 통한 D-리모넨의 멜라닌 생성 촉진: 메커니즘 및 시사점

D-limonene Enhances Melanogenesis via the MAPK/Akt Signaling Pathway: Mechanisms and Implications

  • 김찬용 (경희대학교 생명공학원 유전생명공학과) ;
  • 김용욱 (경희대학교 생명공학원 유전생명공학과) ;
  • 조해여 (경희대학교 생명공학원 유전생명공학과) ;
  • 황재성 (경희대학교 생명공학원 유전생명공학과)
  • Chan Yong Kim (Department of Genetics & Biotechnology, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Yong Wook Kim (Department of Genetics & Biotechnology, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Hai Ru Zhao (Department of Genetics & Biotechnology, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Jae Sung Hwang (Department of Genetics & Biotechnology, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
  • 투고 : 2025.03.12
  • 심사 : 2025.03.27
  • 발행 : 2025.03.30
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초록

멜라닌합성반응(melanogenesis)는 티로시나제(TYR) 및 티로시나아제 연관 단백질(TYRP-1, TYRP-2)의 효소적 반응을 통해 멜라닌을 합성하는 과정으로, 합성된 멜라닌은 멜라노좀을 통해 각질형성세포로 전달된다. 이 과정에서 미세소관 조절 전사인자 MITF는 TYR, TYRP-1, TYRP-2의 발현을 조절하여 멜라닌 생성을 촉진하는 핵심 역할을 한다. D-리모넨은 다양한 연구에서 항염, 항산화, 항암효과를 보인것으로 보고되었다. 이전 연구에서는 2.5% D-리모넨을 함유한 Alpinia nantoensis 정유가 포스콜린에 의해 유도된 멜라노합성반응를 저해한다고 보고된 바 있다. 본 연구에서는 50 µM D-리모넨 처리 시 멜라닌생성세포의 멜라닌 함량이 20% 이상 증가함을 확인하였다. 또한, D-리모넨 농도에 따라 MITF, TYR, TRP1, TRP2 등 멜라노 제네시스 관련 유전자 발현이 농도 의존적으로 증가하는 것이 관찰되었다. 더 나아가, D-리모넨이 MAPK/Akt/CREB 신호 전달 경로를 활성화 하며, 이는 p38, JNK, Akt, CREB의 인산화 증가를 통해 입증되었다.

Melanogenesis is the process of melanin synthesis through the enzymatic reactions of tyrosinase (TYR) and tyrosinase-related proteins (TYRP-1, TYRP-2). The synthesized melanin is then transferred to keratinocytes via melanosomes. In this process, the microphthalmia-associated transcription factor (MITF) plays a key role in promoting melanin production by regulating the expression of TYR, TYRP-1, and TYRP-2. D-limonene has demonstrated anti-inflammatory, antioxidant, and anti-cancer properties in various studies. Previous research has indicated that the essential oils of Alpinia nantoensis, which contain 2.5% D-limonene, inhibit forskolin-induced melanogenesis. In the present study, we showed that treatment with 50 µM D-limonene resulted in a greater than 20% increase in melanin content in melanocytes. Notably, the concentration of D-limonene exhibited a dose-dependent effect on the upregulation of melanogenesis-related genes, including MITF, TYR, TRP1, and TRP2. Furthermore, we demonstrated that D-limonene activates the MAPK/Akt/CREB signaling pathway, as evidenced by the enhanced phosphorylation of p38, JNK, Akt and CREB.

키워드

과제정보

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: RS-2023-KH136470).

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