대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제51권1호
  • /
  • Pages.67-73
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  • 2025
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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덤불모자반 추출물의 멜라닌 생성 억제 효과

Inhibitory Effect of Sargassum yezoense Extract on Melanin Synthesis

  • 심중현 (서울여자대학교 바이오헬스융합학과)
  • Joonghyun Shim (Department of Biohealth-Convergence, Seoul Women's University)
  • 투고 : 2025.03.06
  • 심사 : 2025.03.18
  • 발행 : 2025.03.30
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초록

덤불모자반 추출물이 B16F10 세포주에 미치는 세포 독성, 티로시나아제 활성, 티로시나아제 및 TYRP1/2, MITF 유전자 발현 및 멜라닌 생성을 분석하였다. 실시간 유전자 중합효소 연쇄반응 분석결과, 덤불모자반 추출물을 처리한 실험군은 α-MSH 처리군에 비해 TYR, TYRP1, TYRP2 및 MITF의 mRNA 발현 수준을 감소시켰다. 티로시나아제 활성 분석 결과, 덤불모자반 추출물은 B16F10 세포주에서 멜라닌 합성을 감소시키는 것으로 나타났습니다. 이러한 결과는 덤불모자반 추출물이 B16F10 세포에 미치는 효과를 통하여 멜라닌 생성을 억제시키는 소재임을 보여준다.

This study was performed to validate the inhibitory effects of Sargassum yezoene (S. yezoense) extract on melanin synthesis by measuring cell viability, tyrosinase activity, gene expressions, and melanogenesis in the B16F10 cell line. The effects of S. yezoense extract on tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1), TYRP2, and microphthalmia-associated transcription factor (MITF) gene expression levels and melanin synthesis were measured. Quantitative real-time (qRT)-PCR showed that S. yezoense extract decreased the mRNA expression levels of TYR, TYRP1, TYRP2, and MITF, resulting in reduce melanogenesis compared to α-MSH-treated B16F10 cells. Tyrosinase activity assays reveal that S. yezoense extract decrease melanin synthesis in B16F10 cell line. These results demonstrate the whitening effects of S. yezoense extract on B16F10 cells, providing that S. yezoense extract is a potent raw material for skin whitening. Further research is required on the mechanism of S. yezoense extract on the melanogenesis. Such research will benefit not only the cosmetic, but also the dietary supplements and medical industries.

키워드

과제정보

이 논문은 서울여자대학교 학술연구비의 지원에 의한 것임(2025-0027).

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