대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제51권1호
  • /
  • Pages.23-34
  • /
  • 2025
  • /
  • 1226-2587(pISSN)
  • /
  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
권호 표지
DOI QR코드

DOI QR Code

유자씨 오일의 멜라닌 생성 저해 및 항노화 효과에 관한 연구

Study on the Melanin Production Inhibition and Anti-aging Effects of Yuzu (Citrus junos) Seed Oil

  • 이보배 (전라남도농업기술원 과수연구소) ;
  • 박윤하 (전남대학교 융합식품바이오공학과) ;
  • 정현주 (전라남도농업기술원 과수연구소) ;
  • 김대선 (전라남도농업기술원 과수연구소) ;
  • 김병삼 (전라남도농업기술원 과수연구소) ;
  • 남승희 (전남대학교 농업생명과학대학)
  • Bo Bae Lee (Fruit Research institute of Jeollanamdo Agricultural Research and Extension Services) ;
  • Yun Ha Bak (Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University) ;
  • Hyeon Ju Jeong (Fruit Research institute of Jeollanamdo Agricultural Research and Extension Services) ;
  • Dae Seon Kim (Fruit Research institute of Jeollanamdo Agricultural Research and Extension Services) ;
  • Byeong Sam Kim (Fruit Research institute of Jeollanamdo Agricultural Research and Extension Services) ;
  • Seung Hee Nam (Institute of Agricultural Science and Technology, Chonnam National University)
  • 투고 : 2025.02.18
  • 심사 : 2025.03.20
  • 발행 : 2025.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 대부분 폐기 처분되어 활용되지 않는 유자씨의 활용도를 높이기 위하여, 냉압착 방법으로 추출한 유자씨 오일의 이화학적 특성, 멜라닌 생성 저해 및 항노화 효과에 관한 연구를 수행하였다. 유지에서 가장 중요하게 생각하는 부분인 산화안정성에서는 유자씨를 냉풍건조한 시료가 자연건조한 시료보다 thiobarbituric acid (TBA) 값이 약 30% 감소한 것으로 나타나 산화안정성이 다소 높은 것으로 확인되었다. 유자씨 오일은 주로 불포화지방산인 oleic acid와 linoleic acid로 이루어졌고 약 74% 함유하였다. 유자씨 오일과 유자씨 오일이 함유된 미스트의 미백 효과를 확인하기 위해 세포 내 tyrosinase 활성, 멜라닌 함량을 시험하였다. 그 결과 유자씨 오일의 함량이 증가할수록 tyrosinase 활성 및 멜라닌 함량이 감소하는 경향을 나타내었고 특히, 유자씨 오일이 5% 함유된 미스트 0.05%와 0.1% 처리 시 멜라닌 생성량은 각각 145.83%, 139.23%로 나타냈으며, 이는 MSH 군 대비 hydroquinone 50 µM 처리군의 멜라닌 억제능의 40% 수준에 해당하였다. 유자씨 오일을 함유한 유자미스트가 MMP-1 생성을 저해하고, pro-collagen 및 히알루론산 합성을 촉진하는 항주름 기능성 제품의 가능성을 시사하며 피부 미용 성분으로써 유자씨 오일의 활용에 대한 높은 가능성을 확인하였다.

In this study, in order to increase the utilization of yuzu (Citrus junos) seeds, which are mostly discarded and not utilized, the physicochemical properties, melanin production inhibition, and anti-aging effects of yuzu seed oil extracted by the cold pressing method. In terms of oxidation stability, which is the most important part of oil, the TBA value decreased by about 30% in the cold air drying yuzu seed sample compared to the naturally dried sample, indicating that the oxidation stability was somewhat higher. The yuzu seed oil mainly consisted of oleic acid and linoleic acid, which are unsaturated fatty acids, and contained about 74%. In order to confirm the whitening effect of the mist containing yuzu seed oil and yuzu seed oil, intracellular tyrosinase activity and melanin content were tested. As a result, the tyrosinase activity and melanin content tended to decrease as the yuzu seed oil content increased, and in particular, when treated with 0.05% and 0.1% yuzu seed oil, the melanin production was 145.83% and 139.23%, respectively, which was 40% of the melanin inhibitory capacity of the hydroquinone 50 µM treatment group compared to the MSH group. Yuza mist containing yuzu seed oil inhibits MMP-1 production, suggests the possibility of anti-wrinkle functional products that promote procollagen and hyaluronic acid synthesis, and confirms the high possibility of using yuzu seed oil as a skin care component.

키워드

과제정보

본 연구는 지역특화작목기술개발 사업인 「전남 특화 유자 수출 경쟁력 향상을 위한 업사이클링 기술개발 및 산업화(RS-2024-00437307)」 연구비에 의하여 수행된 결과의 일부로, 이에 깊은 감사를 드립니다.

참고문헌

  1. J. E. Lee and S. Y. Park, Review of the usability of cylodextrin as a cosmetic ingredient, Asian J. Beauty Cosmetol, 17(4), 545 (2019). https://doi.org/10.20402/ajbc.2019.0314
  2. S. H. Hwang and G. N. Kim, Antioxidant and whitening effects of Lotus (Nelumbo nucifera) seeds extract through tyrosinase inhibition, J. East Asian Soc. Diet. Life, 33(3), 233 (2023). https://doi.org/10.17495/easdl.2023.6.33.3.233
  3. J. H. Kim, S. J. Yoon, K. H. Lee, H. J. Kwon, S. S. Chun, T. W. Kim, and Y. J. Cho, Screening of biological activities of the extracts from Bisil (Ocimum basilicum L.), Appl. Biol. Chem, 48(2), 173 (2005).
  4. K. H. Chung, J. A. Yoon, B. C. Song, and J. H. An, Free radical-scavenging activities of Amaranth (Amaranthus spp. L.) seed extracts, Food Eng. Prog, 18(2), 116 (2014). https://doi.org/10.13050/foodengprog.2014.18.2.116
  5. S. C. Jeong, J. H. Park, and J. H. Kim, The development trend of skin beauty food with skin protection effects from natural source, Kor. J. Aesthet. Cosmetol, 11(2), 203 (2013).
  6. H. S. Lee and M. S. Jang, A study on quality characteristics andstorage of Julpyun affected by Chungmirae (Smilax china L) leaf powder, Korean J Food Cook Sci, 21(4), 482 (2005).
  7. G. Block and L. Langseth, Antioxidant vitamins and disease prevention, Food Technol., 48(7), 80 (1994).
  8. S. K. Kang, M. J. Jang, and Y. D. Kim, A study on the flavor constiuents of the citron (Citrus junos), Korean J. Food Preserv, 13(2), 204 (2006).
  9. Y. J. Lee, I. G. Hwang, E. M. Joung, H. Y. Kim, E. S. Park, K. S. Woo, and H. S. Jeong, Physiological activity and antiproliferation effects of citron seed extracts on cancer cells, J. Korean Soc. Food Sci. Nutr, 38(12), 1672 (2009). https://doi.org/10.3746/jkfn.2009.38.12.1672
  10. J. H. Shin, S. J. Lee, J. K. Seo, E. W. Cheon, and N. J. Sung, Antioxidant activity of hot-water extract from Yuza (Citrus junos SIEB ex TANAKA) peel, J. Life Sci, 18(12), 1745 (2008). https://doi.org/10.5352/JLS.2008.18.12.1745
  11. H. S. Yang, S. J. Hwang, S. H. Lee, and J. B. Eun, The fermentation characteristics and sensory characteristics of Makgeolli with dried citron (Citrus junos SIEB ex TANAKA) peel, Korean J. Food Sci. Technol, 43(5), 603 (2011). https://doi.org/10.9721/KJFST.2011.43.5.603
  12. H. W. Nam and Y. H. Hyun, Drying of citron juice from by-product of citron tea manufacturing, Korean J. Food Nutr, 16(4), 334 (2003).
  13. O. C. Kwon, J. H. Shin, M. J. Kang, S. J. Lee, S. Y. Choi, and N. J. Sung, Antioxidant activity of ethanol extracts from citron (Citrus junos SIEB ex TANAKA) seed, J. Korean Soc. Food Sci. Nutr, 35(3), 294 (2006). https://doi.org/10.3746/jkfn.2006.35.3.294
  14. S. J. Lee, S. Y. Choi, J. H. Shin, S. H. Kim, H. C. Lim, and N. J. Sung, Fatty acid composition and oxidative stability of citron seed oils, J. Life Sci, 16(3), 427 (2006). https://doi.org/10.5352/JLS.2006.16.3.427
  15. S. Y. Kim and K. S. Shin, Evaluation of physiological activities of the citron (Citrus junos Sieb. ex TANAKA) seed extracts, Prev. Nutr. Food Sci, 18(3), 196 (2013). https://doi.org/10.3746/pnf.2013.18.3.196
  16. K. S. Woo, J. Y. Jeong, I. G. Hwang, Y. J. Lee, Y. R. Lee, H. J. Park, E. S Park, and H. S. Jeong, Antioxidant activity of ethanol extraction on citron seed by response surface methodology, J Korean Soc. Food Sci. Nutr, 38(3), 384 (2009). https://doi.org/10.3746/jkfn.2009.38.3.384
  17. M. K. Shin, H. H. Oh, and K. T. Hwang, Contents and fatty acid compositions in fats extracted from ice creams and ice cream-related products, J. Korean Soc. Food Sci. Nutr, 35(6), 721 (2006). https://doi.org/10.3746/jkfn.2006.35.6.721
  18. M. J. Wie, J. H. Seong, K. W. Jeon, H. S. Jung, and J. S. Lee, Comparison of vitamin E, phytosterols and fatty acid composition in commercially available grape seed oils in Korea. Korean Soc. Food Sci. Nutr, 37(7), 953 (2008). https://doi.org/10.3746/jkfn.2008.37.7.953
  19. E. A. Ko, S. H. Nam, H. Jeong, B. Y. Kim, S. H. Kwak, S. Kim, I. K. Hong, and H. Kang, Antioxidant, anti-inflammatory and anti-allergenic effects of Citrus junos seed oil and its human skin protection, J. Soc. Cosmet. Sci. Korea, 46(3), 283 (2020).
  20. J. B. Kim, K. H. Kim, S. K. Hwang, Y. H. Kim, K. J. Cho, Y. S. Hwang, and R. D. Park, The composition of useful medium chain fatty acid in eight plant species, J. Korean Soc. Agric. Chem. Biotechnol, 44(1), 20 (2001).
  21. J. Gamazo-vazquez, M. S. Garacia-Falcon, and J. Simal-Gandara, Control of contamination of olive oil by sunflower seed oil in bottling plants by GC-MS of fatty acid methyl esters, Food control, 14(7), 463 (2003). https://doi.org/10.1016/S0956-7135(02)00102-0
  22. S. Y. Choi, J. B. Lee, H. J. Yeom, M. J. Oh, and J. Y. Lee, Verification of whitening activity of Inonotus obliquus extracts in B16F10 melanoma cells, Journal of Life Science, 34(2), 105 (2024). https://doi.org/10.5352/JLS.2024.34.2.105
  23. V. M. Virador, N. Kobayashi, J. Matsunaga, and V. J. Hearing, A standardized protocol for assessing regulators of pigmentation, Analytical Biochemistry, 270(2), 207 (1999). https://doi.org/10.1006/abio.1999.4090
  24. J. E. Park, J. I. Kim, M. H. Lee, S. U. Kim, E. Y. Oh, K. S. Cho, and K. W. Oh, Influence of roasting temperature on the functional components of perilla and sesame oils, J. Korean Soc. Food Sci. Nutr, 50(2), 149 (2021). https://doi.org/10.3746/jkfn.2021.50.2.149
  25. E. O. Cheo, Changes of functional components present in lipid foods during cooking, Korean J. Food Cookery Sci, 21(5), 742 (2005).
  26. J. H. Chiang, F. J. Tsai, T. H. Lin, J. S. Yang, and Y. J. Chiu, Tremella fuciformis inhibits melanogenesis in B16F10 cells and promotes migration of human fibroblasts and keratinocytes, In Vivo, 36(2), 713 (2022). https://doi.org/10.21873/invivo.12757
  27. S. A. D'Mello, G. J. Finlay, B. C. Baguley, and M. E. Askarian-Amiri, Signaling pathways in melanogenesis, Int. J. Mol. Sci., 17(7), 1144 (2016). https://doi.org/10.3390/ijms17071144
  28. C. Montoya, L. González, S. Pulido, L. Atehortúa, and S. M. Robledo, Identification and quantification of limonoid aglycones content of Citrus seeds, Revista Brasileira de Farmacognosia, 29(6), 710 (2019). https://doi.org/10.1016/j.bjp.2019.07.006
  29. M. H. Choi, S. H. Yang, N. D. Kim, and H. J. Shin, Nomilin from Yuzu seed has in vitro antioxidant activity and downregulates melanogenesis in B16F10 melanoma cells through the PKA/CREB signaling pathway, Antioxidants, 11(9), 1636 (2022). https://doi.org/10.3390/antiox11091636
  30. J. H. Yoon, S. H. Park, S. E. Yoon, S. Y. Hong, J. B. Lee, J. Lee, and J. Y. Cho, Hydrangea serrata hot water extract and its major ingredient hydrangenol improve skin moisturization and wrinkle conditions via AP-1 and Akt/PI3K pathway upregulation, Nutrients, 15(11), 2436 (2023). https://doi.org/10.3390/nu15112436
  31. J. W. Hong, S. H. Yeom, Y. S. Oh, J. H. Park, M. H. Kang, Y. H. Kim, Z. Kim, K. J. Yim, and J. W. Kim, Enhancement of collagen synthesis through the regulations of MMP-1, COL1A1, and SMAD-3 by extract of the freshwater microalgae Mychonastes sp. 249, J. Korean Soc. Food Sci. Nutr, 52(2), 203 (2023). https://doi.org/10.3746/jkfn.2023.52.2.203
  32. J. Varani, R. L. Warner, M. Gharaee-Kermani, S. H. Phan, S. Kang, J. Chung, Z. Q. Wang, S. C. Datta, G. J. Fisher, and J. J. Voorhees, Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin, J. Invest Dermatol, 114(3), 480 (2000). https://doi.org/10.1046/j.1523-1747.2000.00902.x
  33. J. K. Kim, J. H. Lee, M. S. Yang, D. B. Seo, and S. J. Lee, Beneficial effect of collagen peptide supplement on anti-aging against photodamage, Korean J. Food Sci. Technol, 41(4), 441 (2009).
  34. J. Necas, L. Bartosikova, P. Brauner, and J. Kolar, Hyaluronic acid (hyaluronan): a review, Veterinarni medicina, 53(8), 397 (2008).
  35. M. J. Wie, S. H. Jang, M. R. Jeong, J. M. Yoon, H. S. Jeong, and J. S. Lee, Analysis of phytosterol and fatty acid compositions of grape seeds produced in Korea, J. Korean Soc. Food Sci. Nutr, 38(4), 525 (2009). https://doi.org/10.3746/jkfn.2009.38.4.525