Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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An Amphibians-Derived Protein Provides Novel Biotherapeutics for Various Wounds Treatment

  • Hao-Ran Chen (Hangzhou Institute of Innovative Medicine, Institute of Drug Discovery and Design, Zhejiang University) ;
  • Nan Zhou (Hangzhou Institute of Innovative Medicine, Institute of Drug Discovery and Design, Zhejiang University) ;
  • Yu-Da Liu (Hangzhou Institute of Innovative Medicine, Institute of Drug Discovery and Design, Zhejiang University) ;
  • Li-Hua Peng (Hangzhou Institute of Innovative Medicine, Institute of Drug Discovery and Design, Zhejiang University)
  • Received : 2024.06.09
  • Accepted : 2024.10.22
  • Published : 2025.03.01
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Abstract

Acute burns and chronic wounds frequently fail to heal owing to various reasons. Most drugs currently used for wound therapy in clinical practice have notable drawbacks, making their application a substantial concern. For instance, anti-inflammatory drugs can exert multisystem toxicity, and cellular therapies are costly and difficult to retain. In recent years, natural functional proteins derived from animals and plants have gained increasing attention owing to their unique biological activities, low cost, and broad application prospects in wound therapy. Herein, we isolated a new protein (JH015Y) from amphibians and demonstrated its excellent wound repair and regeneration properties compared with those of epidermal growth factor, both in vitro and in vivo. JH015 protein increased the proliferative ability of human keratinocytes and skin fibroblasts by 47.73 and 41.40%, respectively. In vivo, the medium-dose (0.5 mg/dose) groups of JH015Y protein demonstrated accelerated wound healing from day 4, with wound healing rates 1.26, 1.27, and 1.14 times that of the blank group in acute wounds, burn wounds, and diabetic ulcer, respectively. Histological analysis of Masson-stained sections indicated that the JH015Y protein contributed to collagen deposition on the wound surface, markedly reduced inflammatory cell infiltration, and exhibited low biological toxicity. Accordingly, the JH015Y protein is a promising biotherapeutic agent for accelerated wound repair and regeneration.

Keywords

Acknowledgement

The study was supported by the National Natural Science Foundation and National Key Research and Development Program of China (Grant no. 82374043, U23A20505, 2022YFC3501904), Zhejiang Province Commonweal Projects (Grant no. LGF22H280001), China-ASEAN International Innovative Center for Health Industry of Traditional Chinese Medicine (Gui Ke AD20297142).

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