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아반토퍼포먼스머티리얼즈코리아 (Avantor Performance Materials Korea)
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스케일업 프로세스: 분석적 스케일에서 준비적 스케일로 전환하기 위한 LC 프로세스

The Scale-up Process: from Analytical to Preparative Scale LC Separations

  • 발행 : 2024.06.26
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초록

This document explores the methodologies and considerations for scaling up liquid chromatography (LC) processes from analytical to preparative scales. It provides detailed equations and practical guidance for optimizing parameters such as flow rates, injection volumes, and gradient times while maintaining efficiency and resolution. The paper also includes case studies demonstrating the purification of proteins like α-lactalbumin from milk fractions, highlighting variations in purity across different fractions. With a focus on achieving high reproducibility and maximizing yield, this guide serves as a valuable resource for researchers and industry professionals engaged in preparative chromatography.

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참고문헌

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