Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway

  • Yujin Kim (Department of Dermatology, College of Medicine, Chung-Ang University) ;
  • Jung Ok Lee (Department of Dermatology, College of Medicine, Chung-Ang University) ;
  • Jung Min Lee (Department of Dermatology, College of Medicine, Chung-Ang University) ;
  • Mun-Hoe Lee (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Hyeong-Min Kim (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Hee-Chul Chung (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Do-Un Kim (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Jin-Hee Lee (Health Food Research and Development, NEWTREE Co., Ltd.) ;
  • Beom Joon Kim (Department of Dermatology, College of Medicine, Chung-Ang University)
  • Received : 2023.08.09
  • Accepted : 2023.09.21
  • Published : 2024.01.28
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Abstract

Low molecular weight collagen peptide (LMWCP) is a collagen hydrolysate derived from fish. We investigated the effects of LMWCP on hair growth using human dermal papilla cells (hDPCs), human hair follicles (hHFs), patch assay, and telogenic C57BL/6 mice, while also examining the underlying mechanisms of its action. LMWCP promoted proliferation and mitochondrial potential, and the secretion of hair growth-related factors, such as EGF, HB-EGF, FGF-4, and FGF-6 in hDPCs. Patch assay showed that LMWCP increased the neogeneration of new HFs in a dose-dependent manner. This result correlated with an increase in the expression of dermal papilla (DP) signature genes such as, ALPL, SHH, FGF7, and BMP-2. LMWCP upregulated phosphorylation of glycogen synthase kinase-3β (GSK-3β) and β-catenin, and nuclear translocation of β-catenin, and it increased the expression of Wnt3a, LEF1, VEGF, ALP, and β-catenin. LMWCP promoted the growth of hHFs and increased the expression of β-catenin and VEGF. Oral administration of LMWCP to mice significantly stimulated hair growth. The expression of Wnt3a, β-catenin, PCNA, Cyclin D1, and VEGF was also elevated in the back skin of the mice. Furthermore, LMWCP increased the expression of cytokeratin and Keratin Type I and II. Collectively, these findings demonstrate that LMWCP has the potential to increase hair growth via activating the Wnt/β-catenin signaling pathway.

Keywords

Acknowledgement

This research was supported by NEWTREE Co., Ltd.

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