Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Purification of angiotensin converting enzyme inhibitory peptides and antihypertensive effect generated from Indonesian traditional fermented beef (Cangkuk)

  • Irdha Mirdhayati (Department of Animal Science, Faculty of Agriculture and Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau) ;
  • Wieda Nurwidada Haritsah Zain (Department of Animal Science, Faculty of Agriculture and Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau) ;
  • Abdul Fatah (Department of Animal Science, Faculty of Agriculture and Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau) ;
  • Issei Yokoyama (Department of Animal Science, School of Veterinary Medicine, Kitasato University) ;
  • Keizo Arihara (Department of Animal Science, School of Veterinary Medicine, Kitasato University)
  • Received : 2023.10.21
  • Accepted : 2024.04.08
  • Published : 2024.10.01
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Abstract

Objective: Traditional fermented meat products can be considered a source of bioactive peptides. Cangkuk, a traditional Indonesian fermented beef product is one source of angiotensin converting enzyme (ACE) inhibitory peptides. This study aimed to identify ACE-inhibitory peptides from Cangkuk and analyze their antihypertensive effects. Methods: The water-soluble fraction of Cangkuk was fractionated to obtain ACE-inhibitory peptides using an ethanol solvent at several concentrations and solid-phase extraction with an OASIS HLB cartridge followed by purification with reversed-phase high-performance liquid chromatography (RP-HPLC). HPLC-MS was used to identify target peptides, followed by automatic protein sequencer analysis to detect peptide sequences. Antihypertensive effects were analyzed on the water-soluble fraction and synthesized peptides. The animal model comprised 14-16-week-old male spontaneously hypertensive rats (SHRs) (~320 g average body weight) with mean systolic blood pressures (SBPs) higher than 190 mmHg. All oral doses of peptides were 1 mL in volume. Distilled water was used as a control. The antihypertensive activities of the sample and control were observed by measuring the SBP at 0, 2, 4, 6, 8 and 24 h after oral administration. Results: Two sequences of ACE inhibitory peptides were found, EAPLNPKANR (IC50 value of 44.6 µmol/L) and IVG (IC50 value of 97.3 µmol/L). The water-soluble fraction demonstrated an antihypertensive effect on SHRs after oral administration at 100 mg/kg body weight, maximally lowering the SBP by 14.9 mmHg 8 h after administration. The tripeptide IVG showed the highest reduction of SBP, 24.76±2.1 mmHg 8 h after administration. The decapeptide EAPLNPKANR showed the highest reduction of SBP, 21.0±1.9 mmHg, 8 h after administration. All the samples differed significantly from the control (p<0.01). Conclusion: Cangkuk has potential as a functional food ingredient acting as an antihypertensive agent.

Keywords

Acknowledgement

This research was financially supported by the Directorate of Islamic Higher Education of the Ministry of Religious Affairs Republic of Indonesia through the program of Global Applied Research 2019, Contract Number: 1967 and Laboratory of Food Function and Safety, School of Veterinary Medicine, Kitasato University, Japan.

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