Pediatric Gastroenterology, Hepatology & Nutrition

  • 제27권6호
  • /
  • Pages.345-354
  • /
  • 2024
  • /
  • 2234-8646(pISSN)
  • /
  • 2234-8840(eISSN)
대한소아소화기영양학회 (The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition)
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Expression of IL-7RαlowCX3CR1+ CD8+ T Cells and α4β7 Integrin Tagged T Cells Related to Mucosal Immunity in Children with Inflammatory Bowel Disease

  • Da Hee Yang (Graduate School, Jeju National University College of Medicine) ;
  • Hyo Jin Kim (Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Duong Thi Thuy Dinh (Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Jiwon Yang (Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Chang-Lim Hyun (Department of Pathology, Jeju National University College of Medicine) ;
  • Youngheun Jee (Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Naeun Lee (Center for Integrative Rheumatoid Transcriptomis and Dynamics, The Catholic University of Korea) ;
  • Min Sun Shin (Department of Internal Medicine, Yale School of Medicine) ;
  • Insoo Kang (Department of Internal Medicine, Yale School of Medicine) ;
  • Ki Soo Kang (Department of Pediatrics, Jeju National University College of Medicine)
  • 투고 : 2024.05.30
  • 심사 : 2024.10.01
  • 발행 : 2024.11.15
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초록

Purpose: The study aimed to investigate the recruiting of T lymphocytes including IL-7Rαlow CX3CR1+ effector memory (EM) CD8+ T cells and α4β7 integrin tagged T cells to inflamed intestinal mucosa. Methods: Whole blood and mucosal tissues of intestine were collected from 40 children with or without inflammatory bowel disease (IBD). T cell surface staining and immunohistochemistry were done with several antibodies in peripheral blood mononuclear cells (PBMCs) and intestinal mucosa, respectively. Serum levels of cytokines were measured by ELISA. Results: The frequency of IL-7RαlowCX3CR1+ EM CD8+ T cells in the PBMC was significantly higher in the ulcerative colitis group than in the control group (57.9±17.80% vs. 33.9±15.70%, p=0.021). The frequency of integrin α4β7+ CD4+ T cells in the PBMC was significantly lower in the ulcerative colitis group than in the control group (53.2±27.6% vs. 63.9±13.2%, p=0.022). Serum concentration of TNF-α was higher in the Crohn's disease group than in the control group (26.13±5.01 pg/mL vs. 19.65±6.07 pg/mL, p=0.008). Of the three groups, the ulcerative colitis group had the highest frequency of integrin α4β7+ T cells based on immunohistochemistry analyses for intestinal tissues, followed by the Crohn's disease group and the control group (4.63±1.29 cells vs. 2.0±0.57 cells vs. 0.84±0.52 cells, p<0.001). Conclusion: Trafficking immune cells with effector memory CD8+ T cells clarified by IL-7RαlowCX3CR1+ and integrin α4β7+ CD4+ T cells might be highly associated with the pathogenesis of ulcerative colitis.

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과제정보

This work was supported by the Research Funds of Jeju National University Hospital (JNUH-2017) and the National Research Foundation of Korea grant funded by the Korea Government (NRF-2019R1F1A1063400).

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