Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Activity of Sparassis latifolia on the Lipid Accumulation through Suppressing Adipogenesis and Activating Lipolysis in 3T3-L1 Cells

  • Jeong Won Choi (Department of Forest Science, Andong National University) ;
  • Hyeok Jin Choi (Department of Forest Science, Andong National University) ;
  • Rhim Ryoo (Department of Forest Bioresources, Division of Forest Microbiology, National Institute of Forest Science) ;
  • Youngki Park (Department of Forest Bioresources, Division of Forest Microbiology, National Institute of Forest Science) ;
  • Kyoung Tae Lee (Department of Forest Bioresources, Division of Forest Microbiology, National Institute of Forest Science) ;
  • Jin Boo Jeong (Department of Forest Science, Andong National University)
  • Received : 2024.04.23
  • Accepted : 2024.07.22
  • Published : 2024.10.28
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Abstract

Sparassis latifolia (SL) has been reported to exhibit anti-obesity effects in high-fat diet animal models, yet research into its mechanisms of action remains limited. Therefore, this study aimed to elucidate the mechanisms behind the anti-obesity activity of SL's 30% ethanol extract (SL30E) using 3T3-L1 cells in an in vitro setting. SL30E effectively mitigated the accumulation of lipid droplets and triacylglycerol. SL30E downregulated PPARγ and CEBPα protein levels. The diminishment of PPARγ and C/EBPα, facilitated by SL30E, was impeded by the knockdown of β-catenin using β-catenin-specific siRNA. Furthermore, SL30E was observed to increase the protein levels of ATGL and p-HSL, while it concurrently decreased the protein levels of perilipin-1. SL30E downregulated p62/SQSTM1 protein level and upregulated LC3-II protein level. Moreover, SL30E was demonstrated to elevate the protein levels of p-AMPK and PGC-1α. The results indicate that SL30E inhibits lipid accumulation by suppressing adipogenesis and inducing lipolysis, lipophagy, and thermogenesis in 3T3-L1 cells. These observations provide potential insights into the mechanisms underlying the anti-obesity effects of SL, contributing valuable information to the existing body of knowledge.

Keywords

Acknowledgement

This study was supported by a grant from the National Institute of Forest Science (Grant number: FE0100-2023-04-2023) funded by the Korea Forest Service and the R&D Program for Forest Science Technology (Project No. RS-2024-00405196) provided by the Korea Forest Service (Korea Forestry Promotion Institute).

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