Journal of Microbiology and Biotechnology

  • 제34권10호
  • /
  • Pages.2049-2069
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Natural Inhibitory Treatment of Fungi-Induced Deterioration of Carbonate and Cellulosic Ancient Monuments: Isolation, Identification and Simulation of Biogenic Deterioration

  • Mohamed H. El-Sayed (Department of Biology, College of Sciences and Arts-Rafha, Northern Border University) ;
  • Maha AlHarbi (Department of Biology, College of Science, Princess Nourah bint Abdulrahman University) ;
  • Islam A. Elsehemy (Department of Natural and Microbial Products Chemistry, Pharmaceutical Industry Division, National Research Centre) ;
  • Wafaa M. Haggag (Department of Plant Pathology, National Research Centre) ;
  • Bahgat M. Refaat (Department of Botany and Microbiology, Faculty of Science, Al-Azhar University) ;
  • Sharaf M. Ali (Central Research Laboratory, National Museum of Egyptian Civilization, Ministry of Antiquities) ;
  • Amr Elkelish (Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University)
  • 투고 : 2024.04.22
  • 심사 : 2024.08.26
  • 발행 : 2024.10.28
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초록

Fungi play a significant role in the deterioration of various types of monuments. Therefore, the protection of ancient monuments from fungal attacks is an important goal that must attract the attention of researchers worldwide. A total of 69 fungal isolates were recovered from 22 deteriorated objects compromising paper, textiles, wood, and stone in the National Museum of Egyptian Civilization (NMEC) storeroom, Cairo, Egypt. The isolates were identified as 12 different species categorized into three different genera, namely, Aspergillus (9 species), Penicillium (2 species) and Trichoderma (1 species). Among them, Aspergillus fumigatus was the most prevalent species. Three essential oils were assessed for antifungal activity and compared with the antifungal effects of five synthetic microcides to identify a natural inhibitory treatment. Thyme oil and sodium azide were found to be the most active growth inhibitors, with minimum inhibitory concentrations (MICs) of 625 and 100 ppm, with inhibition zone diameters of 19.0 ± 0.70 - 23.76 ± 1.15 and 13.30 ± 0.35 -- 19.66 ± 0.54 mm, respectively. An in vitro simulation of the biodeterioration process was conducted using spores of the A. fumigatus strain NMEC-PSTW.1 on model cubes made of paper, textile, wood, and stone materials. The changes in the characteristics of the artificially deteriorated materials were analyzed using environmental scanning electron microscopy/energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy. The results revealed changes in the morphology, physical properties, and chemical composition induced by A. fumigatus NMEC-PSTW.1. Overall, thyme oil is recommended as a natural inhibitor to protect carbonate and cellulosic monuments in NMEC against fungal attack.

키워드

과제정보

The authors extend their appreciation to the Deanship of Scientific Research at Northern Border University, Arar, KSA, for funding this research work through the project number "NBU-FFR-2024-337-06. The authors also thank the Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2024R182), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

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