Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Carotenoid-Producing Paracoccus aurantius sp. nov., Isolated from the West Coast of Dokdo Island, Republic of Korea

  • Chi Young Hwang (Department of Bioengineering and Nano-Bioengineering, Incheon National University) ;
  • Eui-Sang Cho (Department of Bioengineering and Nano-Bioengineering, Incheon National University) ;
  • Eun Hee Bae (Climate Change and Environmental Biology Research Division, National Institute of Biological Resources) ;
  • Dong-Hyun Jung (Climate Change and Environmental Biology Research Division, National Institute of Biological Resources) ;
  • Myung-Ji Seo (Department of Bioengineering and Nano-Bioengineering, Incheon National University)
  • Received : 2024.04.30
  • Accepted : 2024.07.25
  • Published : 2024.10.28
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Abstract

In this study, a novel species within the genus Paracoccus was isolated from the coastal soil of Dokdo (Seodo) Island and investigated. We elucidated the novel species, designated MBLB3053T, through genomic analysis of novel functional microbial resources. Cells were gram-negative, non-motile, and coccoid, and the colony was light orange in color. Phylogenetic analysis based on the 16S rRNA gene showed that strain MBLB3053T was related to the genus Paracoccus, with 98.5% similarity to Paracoccus aestuariivivens. Comparative genome analysis also revealed the strain to be a novel species of the genus Paracoccus by average nucleotide identity and in silico DNA-DNA hybridization values. Through secondary metabolite analysis, terpene biosynthetic gene clusters associated with carotenoid biosynthesis were found in strain MBLB3053T. Using high-performance liquid chromatography, strain MBLB3053T was confirmed to produce carotenoids, including all-trans-astaxanthin, by comparison to the standard compound. Notably, the isolate was also confirmed to produce carotenoids that other closely related species did not produce. Based on this comprehensive polyphasic taxonomy, strain MBLB3053T represents a novel species within the genus Paracoccus, for which the name Paracoccus aurantius sp. nov is proposed. The type strain was MBL3053T (=KCTC 8269T =JCM 36634T). These findings support the research and resource value of this novel species, which was isolated from the Dokdo environmental microbiome.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202227102 and NIBR202331102).

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