Mycobiology

  • 제52권1호
  • /
  • Pages.42-50
  • /
  • 2024
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Cloning and Expression Analysis of Bioluminescence Genes in Omphalotus guepiniiformis Reveal Stress-Dependent Regulation of Bioluminescence

  • Mi-Jeong Park (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science) ;
  • Eunjin Kim (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science) ;
  • Min-Jun Kim (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science) ;
  • Yeongseon Jang (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science) ;
  • Rhim, Ryoo (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science) ;
  • Kang-Hyeon Ka (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
  • 투고 : 2023.08.29
  • 심사 : 2024.01.03
  • 발행 : 2024.01.31
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초록

Bioluminescence is a type of chemiluminescence that arises from a luciferase-catalyzed oxidation reaction of luciferin. Molecular biology and comparative genomics have recently elucidated the genes involved in fungal bioluminescence and the evolutionary history of their clusters. owever, most studies on fungal bioluminescence have been limited to observing the changes in light intensity under various conditions. o understand the molecular basis of bioluminescent responses in Omphalotus guepiniiformis under different environmental conditions, we cloned and sequenced the genes of hispidin synthase, hispidin-3-hydroxylase, and luciferase enzymes, which are pivotal in the fungal bioluminescence pathway. ach gene showed high sequence similarity to that of other luminous fungal species. Furthermore, we investigated their transcriptional changes in response to abiotic stresses. Wound stress enhanced the bioluminescence intensity by increasing the expression of bioluminescence pathway genes, while temperature stress suppressed the bioluminescence intensity via the non-transcriptional pathway. Our data suggested that O. guepiniiformis regulates bioluminescence to respond differentially to specific environmental stresses. o our knowledge, this is the first study on fungal bioluminescence at the gene expression level. Further studies are required to address the biological and ecological meaning of different bioluminescence responses in changing environments, and O. quepiniiformis could be a potential model species.

키워드

과제정보

This work was supported by a grant from the General Project (FP0800-2023-01) provided by the National Institute of Forest Science, Republic of Korea.

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