환경생물 (Korean Journal of Environmental Biology)

  • 제42권3호
  • /
  • Pages.288-300
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  • 2024
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  • 1226-9999(pISSN)
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  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
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A report on 22 unrecorded Actinomycetota species isolated from freshwater environments in the Republic of Korea

  • Soo-Yeong Lee (Biological Resources Research Department, Nakdonggang National Institute of Biological Resources (NNIBR)) ;
  • Jaeduk Goh (Biological Resources Research Department, Nakdonggang National Institute of Biological Resources (NNIBR)) ;
  • Ahyoung Choi (Biological Resources Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
  • 투고 : 2024.07.12
  • 심사 : 2024.09.10
  • 발행 : 2024.09.30
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초록

Freshwater environments are rich ecosystems that support diverse microbial communities, including members of the phylum Actinomycetota critical for nutrient cycling, organic matter decomposition, and water quality maintenance. Actinomycetota known to produce numerous bioactive secondary metabolites are valuable in biotechnology, medicine, and agriculture. Despite their significance, the diversity and distribution of Actinomycetota in freshwater habitats, especially in the Republic of Korea, are underexplored. This study aimed to report the isolation and characterization of 22 previously unrecorded bacterial species of Actinomycetota from various freshwater environments in Korea. Using standard dilution plating techniques on six different culture media, 22 bacterial strains were isolated, incubated, and characterized based on colony and cellular morphologies, Gram staining, and biochemical properties. Genomic DNA was extracted and the 16S rRNA gene was sequenced to determine species identity using the EzBioCloud service with a cutoff of 98.7% sequence similarity for classification as unreported species. These strains were phylogenetically diverse, belonging to two classes, ten orders, and eighteen genera. This study enhances our understanding of bacterial diversity in freshwater ecosystems and underscores the importance of exploring microbial diversity in underexplored habitats, potentially leading to discovery of novel bioactive compounds. Findings of this study contribute valuable insights into ecological roles and biotechnological potential of Actinomycetota in freshwater environments.

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과제정보

This study was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR20241103).

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