Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Dietary Mulberry leaf 1-deoxynijirimycin supplementation shortens villus height and improves intestinal barrier in fattening rabbits

  • Shaocong Li (Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Tao Li (Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Zijie Jiang (Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Wenyu Hou (Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Qirui Hou (Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Boris Ramos Serrano (Plant Protein and Bionatural Products Research Center) ;
  • Adileidys Ruiz Barcenas (Plant Protein and Bionatural Products Research Center) ;
  • Yuhua Wang (Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Weiguo Zhao (Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology)
  • Received : 2024.02.23
  • Accepted : 2024.05.20
  • Published : 2024.12.01
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Abstract

Objective: The current study investigated the effects of mulberry 1-deoxynijirimycin (DNJ) on the digestion ability, intestinal morphology, and intestinal barrier of rabbits. Methods: A total of 36 New Zealand White rabbits (male) about 45 days old (mean body weight of 1.05±0.04 kg) were reared and commercial diets were employed, and afterwards divided into three groups (n = 12) with different levels of DNJ extract additive in feed: T0 (0 g/kg), T1 (0.35 g/kg), T2 (0.7 g/kg) for 28 d. Results: The results demonstrated that T2 decreased the average daily gain (p<0.05). T1 and T2 decreased villus height and inflammatory factor levels as compared with T0 (p<0.05). DNJ significantly decreased the content of valeric acid (p<0.05). The content of acetic acid, propionic acid, iso butyric acid, iso valeric acid in T1 were higher than those in T0 and T2 (p<0.05). The content of butyric acid in T2 was lower than it in T0 and T1 (p<0.05). The content of caproic acid was firstly improved then reduced as the DNJ concentration improved (p<0.05). T2 significantly increased the abundance of dgA-11_gut_group and Christensenellaceae_R-7_group while decreased Bacteroide and Ralstonia as compared with T0 (p<0.05). Compared with T0, T1, and T2 significantly improved the gene expression of JAM2, JAM3, mucin4, mucin6 (p<0.05), T1 significantly decreased the expression of occluding while T2 significantly increased (p<0.05), T2 significantly increased the expression of claudin1 and claudin2 (p<0.05). Conclusion: DNJ at high level changed microbiome compositions, inhibited inflammation, and improved intestinal barrier while it decreased the growth performance and shorted villus height in rabbit jejunum by regulating short chain fatty acid compositions in rabbits.

Keywords

Acknowledgement

This work was supported by the National Key R&D Program of China (2021YFE0111100), the Sericulture comprehensive technology integration position (CARS-18-ZJ0207), the Science and Technology Partnership Program, the Ministry of Science and Technology of China (KY202201002), Zhenjiang Science and Technology support project (GJ2021015), earmarked fund for CARS-18, the Key R&D Program of Guangxi (AB23026066), the Crop Germplasm Resources Protection Project of the Agriculture Ministry (111721301354052026), National Infrastructure for Crop Germplasm Resources (NICGR-43), and the Postgraduate Research and Practice Innovation Program of Jiangsu Province (SJCX22_1991).

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