Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Genetic variation analysis of Guanling cattle based on whole-genome resequencing

  • Longxin Xu (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Xin Wang (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Junda Wu (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Hua Wang (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Wenzhang Zhou (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Jing Liu (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Mengmeng Ni (College of Animal Sciences, Guizhou University) ;
  • Kaikai Zhang (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Bo Yu (Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences) ;
  • Ruiyi Lin (College of Animal Sciences, Fujian Agriculture and Forestry University)
  • Received : 2024.03.26
  • Accepted : 2024.06.18
  • Published : 2024.12.01
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Abstract

Objective: The objective of this study was to unravel the genetic traits of Guanling cattle, pinpoint genes advantageous for muscle growth, and lay a foundation for the preservation of genetic diversity and further analysis of regulation mechanism of important economic traits in local cattle breed. Methods: In this study, we sequenced the whole genome of 3 Guanling cattle in Guizhou province using the Illumina HiSeq cBo sequencing platform. And, high- multiplex polymerase chain reaction technology was employed to detect high-quality single nucleotide polymorphism (SNP) sites of other 55 Guanling cattle. Results: Our study identified 166,411 non-synonymous SNPs (nsSNPs) and 42,423 insertions and deletions (indels). Through SNP annotation, gene function enrichment analysis, and comparing with Simmental, Angus, and Limousin cattle, we identified six genes (LEPR, AKAP9, SIX4, SPIDR, PRG4, FASN) which are potentially influential on meat quality traits, playing crucial roles in muscle growth, fat metabolism, and bodily support. We also examined polymorphisms at seven SNP sites in Guanling cattle and found that all seven were in Hardy-Weinberg equilibrium. Conclusion: These findings suggested that these gene sites are stable and widespread in the Guanling cattle population. Our research lays the groundwork for future genetic enhancement and variety identification of Guanling cattle.

Keywords

Acknowledgement

This work were supported by Guizhou Department of Science and Technology (Major Project [2020] 3009, Key Project [2022] 027, General project [2021] No.144 and [2020] No. 1Y075, Basic research effort [2022] No.226) and Guizhou academy of agricultural sciences ("JBGS"[2024]No. 02).

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